Oral Sulfonylurea Hypoglycemic Agents Prevent Ischemic Preconditioning in Human Myocardium

Circulation ◽  
1997 ◽  
Vol 96 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Joseph C. Cleveland ◽  
Daniel R. Meldrum ◽  
Brian S. Cain ◽  
Anirban Banerjee ◽  
Alden H. Harken
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium ◽  
Hypoglycemic Agents

PKC-ε is upstream and PKC-α is downstream of mitoKATP channels in the signal transduction pathway of ischemic preconditioning of human myocardium

2004 ◽  
Vol 287 (5) ◽  
pp. C1418-C1425 ◽  
Author(s):  
Ashraf Hassouna ◽  
Bashir M. Matata ◽  
Manuel Galiñanes
Keyword(s):  
Ischemic Preconditioning ◽  
Signal Transduction Pathway ◽  
Human Myocardium ◽  
Right Atrial ◽  
Elective Cardiac Surgery ◽  
Simulated Ischemia ◽  
Pkc Isoforms ◽  
Pkc Inhibitors ◽  
To Receive ◽  
Scion Image

Protein kinase C (PKC) is involved in the process of ischemic preconditioning (IPC), although the precise mechanism is still a subject of debate. Using specific PKC inhibitors, we investigated which PKC isoforms were involved in IPC of the human atrial myocardium sections and to determine their temporal relationship to the opening of mitochondrial potassium-sensitive ATP (mitoKATP) channels. Right atrial muscles obtained from patients undergoing elective cardiac surgery were equilibrated and then randomized to receive any of the following protocols: aerobic control, 90-min simulated ischemia/120-min reoxygenation, IPC using 5-min simulated ischemia/5-min reoxygenation followed by 90-min simulated ischemia/120-min reoxygenation and finally, PKC inhibitors were added 10 min before and 10 min during IPC followed by 90-min simulated ischemia/120-min reoxygenation. The PKC isoforms inhibitors investigated were V1–2 peptide, GO-6976, rottlerin, and LY-333531 for PKC-ε, -α, -δ and -β, respectively. To investigate the relation of PKC isoforms to mitoKATP channels, PKC inhibitors found to be involved in IPC were added 10 min before and 10 min during preconditioning by diazoxide followed by 90-min simulated ischemia/120-min reoxygenation in a second experiment. Creatine kinase leakage and methylthiazoletetrazolium cell viability were measured. Phosphorylation of PKC isoforms after activation of the sample by either diazoxide or IPC was detected by using Western blot analysis and then analyzed by using Scion image software. PKC-α and -ε inhibitors blocked IPC, whereas PKC-δ and -β inhibitors did not. The protection elicited by diazoxide, believed to be via mitoKATP channels opening, was blocked by the inhibition of PKC-α but not -ε isoforms. In addition, diazoxide caused increased phosphorylation of PKC-α to the same extent as IPC but did not affect the phosphorylation of PKC-ε, a process believed to be critical in PKC activation. The results demonstrate that PKC-α and -ε are involved in IPC of the human myocardium with PKC-ε being upstream and PKC-α being downstream of mitoKATP channels.

Ischemic preconditioning is not cardioprotective in senescent human myocardium

2003 ◽  
Vol 76 (1) ◽  
pp. 105-111 ◽  
Author(s):  
Babett Bartling ◽  
Ivar Friedrich ◽  
Rolf-E Silber ◽  
Andreas Simm
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium

scholarly journals Morphine mimics ischemic preconditioning in human myocardium during PTCA

1998 ◽  
Vol 31 ◽  
pp. 65-66 ◽  
Author(s):  
N.P. Xenopoulos ◽  
M. Leesar ◽  
R. Bolli
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium

scholarly journals Effect of Hypoglycemic Agents on Ischemic Preconditioning in Patients With Type 2 Diabetes and Symptomatic Coronary Artery Disease

Diabetes Care ◽  
2012 ◽  
Vol 36 (6) ◽  
pp. 1654-1659 ◽  
Author(s):  
R. M. Rahmi ◽  
A. H. Uchida ◽  
P. C. Rezende ◽  
E. G. Lima ◽  
C. L. Garzillo ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Ischemic Preconditioning ◽  
Hypoglycemic Agents ◽  
Symptomatic Coronary Artery Disease ◽  
Artery Disease

1P-0003 Microcirculation of the human myocardium during ischemic preconditioning

2003 ◽  
Vol 4 (2) ◽  
pp. 19
Author(s):  
A. Lichtenberg ◽  
K. Knobloch ◽  
M. Pichlmaier ◽  
S. Ringes-Lichtenberg ◽  
H. Mertsching ◽  
...  
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium

scholarly journals DIRECT COMPARISON BETWEEN TWO HYPOGLYCEMIC AGENTS: EFFECTS ON MYOCARDIAL ISCHEMIC PRECONDITIONING IN DIABETIC PATIENTS WITH SYMPTOMATIC CORONARY DISEASE

2012 ◽  
Vol 59 (13) ◽  
pp. E1501
Author(s):  
Rosa Maria Rahmi Garcia ◽  
Whady Hueb ◽  
Augusto Uchida ◽  
Paulo Cury Rezende ◽  
Paulo Jorge Moffa ◽  
...  
Keyword(s):  
Coronary Disease ◽  
Ischemic Preconditioning ◽  
Hypoglycemic Agents ◽  
Diabetic Patients

scholarly journals The aging human myocardium: tolerance to ischemia and responsiveness to ischemic preconditioning

2003 ◽  
Vol 126 (1) ◽  
pp. 143-147 ◽  
Author(s):  
Mahmoud Loubani ◽  
Sudip Ghosh ◽  
Manuel Galiñanes
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium

Clinical L-Type Ca2+Channel Blockade Prevents Ischemic Preconditioning of Human Myocardium

1999 ◽  
Vol 31 (12) ◽  
pp. 2191-2197 ◽  
Author(s):  
Brian S Cain ◽  
Daniel R Meldrum ◽  
Joseph C Cleveland, Jr ◽  
Xianzhong Meng ◽  
Anirban Banerjee ◽  
...  
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium ◽  
Channel Blockade

scholarly journals Adenosine Pretreatment of Human Myocardium and Ischemic Preconditioning

Circulation ◽  
1998 ◽  
Vol 97 (22) ◽  
pp. 2279-2279 ◽  
Author(s):  
H. Thomas Lee
Keyword(s):  
Ischemic Preconditioning ◽  
Human Myocardium
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