Ficophagus chaozhouensis n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta Vahl var. roxburghii (Miq.) King in China

Nematology ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 299-312
Author(s):  
Wensheng Zeng ◽  
Dayuan Zhang ◽  
Jianghua Huang ◽  
Yongsan Zeng ◽  
Weimin Ye ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Ficus Hirta ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus hirta var. roxburghii from Chaozhou, Guangdong, China. It is described herein as Ficophagus chaozhouensis n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron (occasionally swollen), absence of gubernaculum (or apophysis), a blunt rosethorn-shaped spicule without a terminal cucullus, and a digitate rostrum with a broadly squared tip. Ficophagus chaozhouensis n. sp. was separated from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that F. chaozhouensis n. sp. is clustered in the same highly supported monophyletic clade with F. aculeata, F. maxima and F. yoponensis, and is sister to F. aculeata.

Martininema semicordatae n. sp. (Nematoda: Aphelenchoididae) associated with Ficus semicordata in China

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 759-770
Author(s):  
Xiulan Zhao ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Common Ancestor ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus semicordata from Xishuangbanna Tropical Botanical Garden, Yunnan Province, China. It is described herein as Martininema semicordatae n. sp. and is characterised by having the combined characters of a long post-vulval uterine sac, excretory pore located at nerve ring level, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron, absence of gubernaculum (= apophysis), and a recurved, rose-thorn-shaped spicule lacking a terminal cucullus. Martininema semicordatae n. sp. differs from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that M. semicordatae n. sp. is clustered in a highly supported monophyletic clade with M. guangzhouense, M. fistulosus and M. baculum, and shares a common ancestor with these three species.

Schistonchus fistulosus n. sp. (Nematoda: Aphelenchoididae) associated with Ficus fistulosa in China

Nematology ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 879-890 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Xinrong Wang ◽  
Zhijian Du ◽  
Robin M. Giblin-Davis
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Tail Tip

A new nematode species was recovered from syconia of Ficus fistulosa from the South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong Province, China, during a survey of nematode biodiversity from 2007 to 2012. It is described herein as Schistonchus fistulosus n. sp. and is typologically characterised by possessing the combined characters of a long post-uterine sac, excretory pore located near the metacorpus, amoeboid sperm, three pairs of subventral papillae on the male tail, a mucron on the male tail tip, absence of a gubernaculum and possession of ox-horn-shaped spicules without a terminal cucullus and with a short, broad and bluntly rounded rostrum. Schistonchus fistulosus n. sp. was easily differentiated from other sequenced species by the partial small subunit rRNA gene (SSU) and D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with partial SSU sequences suggests that S. fistulosus n. sp. has a closer relationship with S. guangzhouensis and S. baculum in the same highly supported monophyletic clade than to the other sequenced Schistonchus species. Based upon inferences using LSU D3 sequence data, it is also in a well-supported monophyletic clade with S. guangzhouensis and S. baculum.

Ficophagus auriculatae n. sp. (Nematoda: Aphelenchoididae), an associate of the Ficus auriculata-complex in China

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 497-508
Author(s):  
Yongsan Zeng ◽  
Jianfeng Huang ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Rongrong Shi ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Small Subunit Rrna Gene ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus auriculata-complex, including F. auriculata, F. oligodon and F. hainanensis, from Xishuangbanna Tropical Botanical Garden, China. It is described herein as Ficophagus auriculatae n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located between the metacorpus and the stylet basal knobs, testis multi-reflexed, amoeboid sperm, three pairs of subventral papillae on male tail, rounded male tail tip without mucron, absence of gubernaculum (= apophysis) and sickle-shaped spicules with terminal cucullus. Ficophagus auriculatae n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analysis with the above-mentioned sequences suggested that F. auriculatae n. sp. has a closer relationship with F. fleckeri in the same highly supported monophyletic clade than with the other sequenced Ficophagus and Schistonchus sensu lato species.

Ficophagus curtipes n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus curtipes in China

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1091-1101
Author(s):  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
Yanqiong Peng ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus curtipes from Xishuangbanna Tropical Botanical Garden, Yunnan, China. It is described herein as Ficophagus curtipes n. sp. and is differentiated from all currently described species in the genus by the possession of a spicule with a ventrally recurved tip (terminal barb). It is further characterised by possessing a short post-uterine sac, an ovoid spermatheca, presence of crustaformeria, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip without mucron, rose-thorn-shaped spicules without cucullus and absence of gubernaculum (= apophysis). Ficophagus curtipes n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analyses suggested that F. curtipes n. sp. has a closer relationship with F. benjamina and F. microcarpus in the same highly supported monophyletic clade than to the other sequenced Ficophagus and Schistonchus s.l. species.

Description of Schistonchus microcarpus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus microcarpa in China

Nematology ◽  
2011 ◽  
Vol 13 (2) ◽  
pp. 221-233 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin Giblin-Davis ◽  
Changhui Li ◽  
Shinian Zhang ◽  
...  
Keyword(s):  
Excretory Pore ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Mtdna Coi ◽  
Ficus Microcarpa ◽  
Tail Tip

AbstractSchistonchus microcarpus n. sp. was recovered from the syconia of Ficus microcarpa from Shenzhen and Guangzhou, Guangdong Province, China, during a survey of nematode biodiversity from 2007 to 2009. It is characterised by possessing the combined characters of a short post-uterine sac (PUS) (3-11 μm or <0.4 vulval body diam. (VBD) long), excretory pore located just posterior to the head but anterior to the conus level of the stylet, prominent amphids, three pairs of subventral papillae on the male tail (one pair adcloacal, one pair halfway between cloaca and tail terminus, and one pair near tail tip), unique recurved and sickleshaped spicules with finely rounded tip with cucullus, amoeboid sperm, and rounded male tail tip with or without mucron. Schistonchus microcarpus n. sp. is morphologically differentiated from all other described species in this genus by the possession of a spicule with a cucullus on the tip. Schistonchus microcarpus n. sp. was easily differentiated from other sequenced species by the partial small subunit rRNA gene (SSU) and D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with partial SSU sequences suggests that S. microcarpus n. sp. is in a highly supported monophyletic clade with sequenced Schistonchus species except for S. hirtus. Based upon inferences using D3 LSU sequence data, it forms a clade with an undescribed species of Schistonchus ex F. benjamini from Australia and is part of a larger clade of Schistonchus that mostly share the character of an anteriorly placed excretory pore. Sequences of partial mtDNA COI (590 bp) from males of S. microcarpus n. sp. with and without a mucronate tail tip were identical, proving that these two morphotypes are conspecific.

Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 543-556 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin M. Giblin-Davis ◽  
Changhui Li ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Wasp Species ◽  
Dna Sequence Data ◽  
Ficus Hirta ◽  
Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

Parasitodiplogaster species associated with Pharmacosycea figs in Panama

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 607-619 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Weimin Ye ◽  
Edward Allen Herre ◽  
Barbara J. Center
Keyword(s):  
Ribosomal Rna ◽  
Morphological Study ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Nominal Species ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Stomatal Morphology ◽  
Rna Genes

Parasitodiplogaster species that are associated with figs in the Ficus subsection Pharmacosycea, i.e., Ficus maxima, F. yoponensis, F. insipida and F. glabrata, were studied using morphological characters and molecular sequences. Some nematodes isolated from the fig sycones were casually observed and recorded as morphospecies according to their morphotype, and were then digested for their DNA, while others were fixed in formalin-glycerin for additional morphological study. High resolution microscopic observation of the morphological materials yielded five morphospecies including two nominal species, P. maxinema and P. pharmaconema. The morphospecies were distinguished from each other by male tail characters. By contrast, five and six genotypes were recognised by D2/D3 expansion segments of the large subunit (LSU) and near-full-length sequences of small subunit (SSU) ribosomal RNA genes, respectively. Except for two nominal species, genotypes and morphospecies were not clearly correlated because of limitations in the microscopic resolution in the initial morphotyping. Although the morphospecies and genotypes were not clearly paired, Pharmacosycea-associated Parasitodiplogaster species, which are tentatively referred to as the ‘P. maxinema group’, formed a monophyletic clade in both D2/D3 LSU and SSU analyses and are morphologically characterised by their stomatal morphology, i.e., a tube-shaped stoma with two stick-like teeth and male tail morphology, presence of nine paired papillae and relatively slender spicule.

Schistonchus zealandicus n. sp. (Nematoda: Aphelenchoididae) associated with Ficus macrophylla in New Zealand

Nematology ◽  
2015 ◽  
Vol 17 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Zengqi Zhao ◽  
Dongmei Li ◽  
Kerrie A. Davies ◽  
Weimin Ye
Keyword(s):  
New Zealand ◽  
Large Subunit ◽  
Tail Length ◽  
Small Subunit ◽  
Rrna Genes ◽  
Lateral Field ◽  
Male Tail ◽  
Tail Tip ◽  
Large Subunit Rrna ◽  
A New Species

Schistonchus zealandicus n. sp. was recovered from the sycones of Ficus macrophylla from St Helier’s Bay, Mt Wellington, and St Johns, suburbs of Auckland, New Zealand. It is characterised by having the opening of the excretory pore located just posterior to the anterior end of the nematode but anterior to the conus level of the stylet, a short post-vulval uterine sac (10-18 μm or 0.4-0.7 vulval body diam. long), presence of two incisures in the lateral field with many broken, non-defined lines between them, rose-thorn-shaped spicule, three pairs of subventral papillae on the male tail (one pair adcloacal on the anterior lip, one pair slightly posterior to mid-tail length, and one pair close to tail tip), a distinctive mucron on the male tail, apparent biogeographical range, and its association with F. macrophylla. The absence of a lip sector disc suggests that it is close to S. altermacrophylla, S. aureus and S. laevigatus. Molecular phylogeny of near full length small subunit and D2-D3 expansion segments of the large subunit rRNA genes supports the proposal of S. zealandicus n. sp. as a new species.

Allodiplogaster josephi n. sp. and A. seani n. sp. (Nematoda: Diplogastridae), associates of soil-dwelling bees in the eastern USA

Nematology ◽  
2015 ◽  
Vol 17 (7) ◽  
pp. 831-863 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Erik J. Ragsdale
Keyword(s):  
New York ◽  
Large Subunit ◽  
Small Subunit ◽  
Valid Species ◽  
Rrna Gene ◽  
Mitochondrial Coi ◽  
Lsu Rrna Gene ◽  
Eastern Usa ◽  
Molecular Characters ◽  
Male Genital

Two commensal associates of bees,Allodiplogaster josephin. sp. from the Dufour’s gland of a cellophane bee (Colletes thoracicus) from Maryland, USA, andA. seanin. sp. from the abdominal glands of an andrenid bee (Andrena alleghaniensis) from New York, USA, are described and illustrated. Both species were collected as dauers from their respective hosts and cultured on bacteria on tryptic soy broth (TSB) or NGM agar.Allodiplogaster josephin. sp. andA. seanin. sp. are morphologically closer to each other than to other species ofAllodiplogaster, which was recently revised to include 37 valid species. However, the two new species are distinguished by reproductive isolation, shape of the spicule manubrium, host associations and molecular characters, the latter in sequences of the near-full length small subunit (SSU) rRNA gene, D2-D3 expansion segments of the large subunit (LSU) rRNA gene and partial mitochondrial COI. Morphological characterisation was supplemented by scanning electron microscopy (SEM), which revealed furcation of both v5 and v6 male genital papillae, consistent with previous reports for species of thehenrichaegroup ofAllodiplogaster.

scholarly journals Nakazawaea Odontotermitis f.a., sp. nov., a Novel Yeast Isolated from the Gut of Odontotermes Horni in India.

2021 ◽  
Author(s):  
Snigdha Tiwari ◽  
Bhaskar C. Behera ◽  
Abhishek Baghela
Keyword(s):  
New Species ◽  
Type Strain ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Its Region ◽  
Small Subunit ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Ascomycetous Yeast ◽  
Lsu Rrna Gene

Abstract Three strains SMT1.3, SMT1.10, and SMT2.2, representing a novel asexual ascomycetous yeast species, were isolated from the gut of a termite Odontotermes horni in Maharashtra, India. Phylogenetic analyses of the LSU, ITS and SSU sequences revealed that they belonged to the genus Nakazawaea, with N. siamensis as the closest relative. The new species differed from the type strain of N. siamensis (DMKU-RK467T) by 1.93% nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, 0.53% nucleotide substitutions in the small subunit (SSU) rRNA gene and 12.6% nucleotide substitutions in the internal transcribed spacer (ITS) region. Notable physiological differences were also observed between N. siamensis and the new species. Hence, the species Nakazawaea odontotermitis f.a., sp. nov. is proposed. The type strain is SMT1.3T (MTCC 13105 = NFCCI 5011). The GenBank accession numbers of the LSU and ITS and SSU sequences of Nakazawaea odontotermitis f.a., sp. nov. are MZ234240, MZ234239 and OK384663. The MycoBank number is MB 841926.

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