Allodiplogaster josephi n. sp. and A. seani n. sp. (Nematoda: Diplogastridae), associates of soil-dwelling bees in the eastern USA

Nematology ◽  
2015 ◽  
Vol 17 (7) ◽  
pp. 831-863 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Erik J. Ragsdale
Keyword(s):  
New York ◽  
Large Subunit ◽  
Small Subunit ◽  
Valid Species ◽  
Rrna Gene ◽  
Mitochondrial Coi ◽  
Lsu Rrna Gene ◽  
Eastern Usa ◽  
Molecular Characters ◽  
Male Genital

Two commensal associates of bees,Allodiplogaster josephin. sp. from the Dufour’s gland of a cellophane bee (Colletes thoracicus) from Maryland, USA, andA. seanin. sp. from the abdominal glands of an andrenid bee (Andrena alleghaniensis) from New York, USA, are described and illustrated. Both species were collected as dauers from their respective hosts and cultured on bacteria on tryptic soy broth (TSB) or NGM agar.Allodiplogaster josephin. sp. andA. seanin. sp. are morphologically closer to each other than to other species ofAllodiplogaster, which was recently revised to include 37 valid species. However, the two new species are distinguished by reproductive isolation, shape of the spicule manubrium, host associations and molecular characters, the latter in sequences of the near-full length small subunit (SSU) rRNA gene, D2-D3 expansion segments of the large subunit (LSU) rRNA gene and partial mitochondrial COI. Morphological characterisation was supplemented by scanning electron microscopy (SEM), which revealed furcation of both v5 and v6 male genital papillae, consistent with previous reports for species of thehenrichaegroup ofAllodiplogaster.

Ficophagus chaozhouensis n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta Vahl var. roxburghii (Miq.) King in China

Nematology ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 299-312
Author(s):  
Wensheng Zeng ◽  
Dayuan Zhang ◽  
Jianghua Huang ◽  
Yongsan Zeng ◽  
Weimin Ye ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Ficus Hirta ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus hirta var. roxburghii from Chaozhou, Guangdong, China. It is described herein as Ficophagus chaozhouensis n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron (occasionally swollen), absence of gubernaculum (or apophysis), a blunt rosethorn-shaped spicule without a terminal cucullus, and a digitate rostrum with a broadly squared tip. Ficophagus chaozhouensis n. sp. was separated from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that F. chaozhouensis n. sp. is clustered in the same highly supported monophyletic clade with F. aculeata, F. maxima and F. yoponensis, and is sister to F. aculeata.

Martininema semicordatae n. sp. (Nematoda: Aphelenchoididae) associated with Ficus semicordata in China

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 759-770
Author(s):  
Xiulan Zhao ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Common Ancestor ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus semicordata from Xishuangbanna Tropical Botanical Garden, Yunnan Province, China. It is described herein as Martininema semicordatae n. sp. and is characterised by having the combined characters of a long post-vulval uterine sac, excretory pore located at nerve ring level, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron, absence of gubernaculum (= apophysis), and a recurved, rose-thorn-shaped spicule lacking a terminal cucullus. Martininema semicordatae n. sp. differs from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that M. semicordatae n. sp. is clustered in a highly supported monophyletic clade with M. guangzhouense, M. fistulosus and M. baculum, and shares a common ancestor with these three species.

scholarly journals Nakazawaea Odontotermitis f.a., sp. nov., a Novel Yeast Isolated from the Gut of Odontotermes Horni in India.

2021 ◽  
Author(s):  
Snigdha Tiwari ◽  
Bhaskar C. Behera ◽  
Abhishek Baghela
Keyword(s):  
New Species ◽  
Type Strain ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Its Region ◽  
Small Subunit ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Ascomycetous Yeast ◽  
Lsu Rrna Gene

Abstract Three strains SMT1.3, SMT1.10, and SMT2.2, representing a novel asexual ascomycetous yeast species, were isolated from the gut of a termite Odontotermes horni in Maharashtra, India. Phylogenetic analyses of the LSU, ITS and SSU sequences revealed that they belonged to the genus Nakazawaea, with N. siamensis as the closest relative. The new species differed from the type strain of N. siamensis (DMKU-RK467T) by 1.93% nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, 0.53% nucleotide substitutions in the small subunit (SSU) rRNA gene and 12.6% nucleotide substitutions in the internal transcribed spacer (ITS) region. Notable physiological differences were also observed between N. siamensis and the new species. Hence, the species Nakazawaea odontotermitis f.a., sp. nov. is proposed. The type strain is SMT1.3T (MTCC 13105 = NFCCI 5011). The GenBank accession numbers of the LSU and ITS and SSU sequences of Nakazawaea odontotermitis f.a., sp. nov. are MZ234240, MZ234239 and OK384663. The MycoBank number is MB 841926.

Teratorhabditis synpapillata Sudhaus, 1985 (Rhabditida: Rhabditidae) is an associate of the red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae)

Nematology ◽  
2008 ◽  
Vol 10 (2) ◽  
pp. 207-218 ◽  
Author(s):  
David Fitch ◽  
Koichi Soné ◽  
Natsumi Kanzaki ◽  
Fukiko Abe ◽  
Robin Giblin-Davis ◽  
...  
Keyword(s):  
Large Subunit ◽  
Agar Plate ◽  
Small Subunit ◽  
Molecular Profile ◽  
Sister Group Relationship ◽  
Cow Dung ◽  
Rrna Gene ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Palm Weevil

AbstractTeratorhabditis synpapillata, originally described from cow dung in Bali, was isolated from the red palm weevil, Rhynchophorus ferrugineus, in Japan. Dauer juveniles were isolated from beneath the elytra of five specimens of R. ferrugineus that had emerged from a dead Canary Island date palm, Phoenix canariensis, in Kagoshima, Japan. The dauer juveniles were cultured on a NGM agar plate and the adult nematodes observed and measured with the aid of light microscopy. The full length of the ribosomal small subunit (SSU) and D2/D3 expansion segment of the ribosomal large subunit (LSU) were sequenced for molecular identification. Based upon morphology, molecular profile and a hybridisation test, the nematode was confirmed as T. synpapillata. A comparison of morphology and morphometrics suggests that the only other nematode reported from R. ferrugineus (= 'Pelodera rhynchophori') is also conspecific with T. synpapillata. A phylogenetic analysis using SSU and LSU rRNA gene sequences fully resolves the relationships of four Teratorhabditis and six outgroup species and demonstrates a sister group relationship of T. synpapillata and T. palmarum, another associate of palm weevils.

Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 393-397 ◽  
Author(s):  
Stephen A. James ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Kathryn Cross ◽  
Christopher J. Bond ◽  
...  
Keyword(s):  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Lsu Rrna Gene

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004T) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004T ( = CBS 12653T = NCYC 3782T) designated as the type strain.

Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽  
2006 ◽  
Vol 1171 (1) ◽  
pp. 17 ◽  
Author(s):  
GUNILLA STÅHLS
Keyword(s):  
Intraspecific Variation ◽  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Large Fragment ◽  
Mitochondrial Coi ◽  
Phylogenetic Placement ◽  
28S Rrna Gene ◽  
Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 543-556 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin M. Giblin-Davis ◽  
Changhui Li ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Wasp Species ◽  
Dna Sequence Data ◽  
Ficus Hirta ◽  
Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

Coleopuccinia in China and its relationship to Gymnosporangium

Phytotaxa ◽  
2018 ◽  
Vol 347 (3) ◽  
pp. 235 ◽  
Author(s):  
BIN CAO ◽  
SIQI TAO ◽  
CHENGMING TIAN ◽  
YINGMEI LIANG
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Rust Fungi ◽  
Internal Transcribed Spacers ◽  
Western China ◽  
Rrna Gene ◽  
Morphological Examination ◽  
Separate Genus ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

The rust species Coleopuccinia sinensis was collected during an investigation of rust fungi in Tibet, a region in south-western China. Through morphological examination, we clarified that C. kunmingensis is a synonym of C. sinensis. Phylogenetic analyses using the combined loci of the internal transcribed spacers (ITS) and the partial large subunit (LSU) rRNA gene revealed that Coleopuccinia should be treated as a separate genus from Gymnosporangium.

Sympodiomycopsis yantaiensis sp. nov., a basidiomycetous yeast isolated from insect frass

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3501-3505 ◽  
Author(s):  
Liang Chen ◽  
Lin Zhang ◽  
Zhi-Hui Li ◽  
Feng-Li Hui
Keyword(s):  
Type Strain ◽  
Large Subunit ◽  
Its Region ◽  
Basidiomycetous Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Sophora Japonica ◽  
Closely Related Species ◽  
D2 Domain ◽  
Lsu Rrna Gene

Two strains (NYNU 121010T and NYNU 121032) of a novel basidiomycetous yeast species belonging to the genus Sympodiomycopsis were isolated from insect frass collected from trunks of a pagoda tree (Sophora japonica L.) in Yantai, Shandong province, east China. The sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the closest relatives were Sympodiomycopsis kandeliae FIRDI 007T, Sympodiomycopsis paphiopedili CBS 7429T and Sympodiomycopsis sp. S6A. The D1/D2 sequences of the novel strains differed by 12 nt substitutions (2 %) from the type strain of S. kandeliae, and by 13 nt substitutions (2.2 %) from the type strain of S. paphiopedili and from Sympodiomycopsis sp. S6A. The novel strains differed from closely related species by more than 4.6 % substitutions in the ITS region. The novel strains can also be distinguished from S. kandeliae and S. paphiopedili on the basis of a number of morphological and physiological characteristics and represent a novel species in the genus Sympodiomycopsis, for which the name Sympodiomycopsis yantaiensis sp. nov. is proposed. The type strain is NYNU 121010T ( = CICC 32998T = CBS 12813T). The Mycobank deposit number is MB 804119.

Characterisation of, and entomopathogenic studies on, Pristionchus aerivorus (Cobb in Merrill & Ford, 1916) Chitwood, 1937 (Rhabditida: Diplogastridae) from North Carolina, USA

Nematology ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 567-580
Author(s):  
Weimin Ye ◽  
Qing Yu ◽  
Natsumi Kanzaki ◽  
Paul R. Adams ◽  
Yasmin J. Cardoza
Keyword(s):  
North Carolina ◽  
Helicoverpa Zea ◽  
Large Subunit ◽  
Small Subunit ◽  
Morphological Observation ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Morphological Studies ◽  
The Status ◽  
Molecular Phylogenetic

During a survey of entomopathogenic nematodes in North Carolina, USA, aPristionchusspecies was recovered using theGalleriabait method. Morphological studies with light microscopy and scanning electron microscopy, mating tests with reference strains, as well as molecular analyses of the near-full-length small subunit rRNA gene (18S) and D2-D3 expansion segments of the large subunit rRNA gene (28S) identified this isolate asPristionchus aerivorus. ExposedGallerialarvae were killed within 48 h and high numbers of nematodes were recovered from the cadavers about 5 days later. Preliminary tests revealed that this nematode is capable of infecting at least two other insect species (Helicoverpa zeaandTenebrio molitor) under laboratory conditions. The status of the genusChroniodiplogasteris discussed and confirmed as a junior synonym ofPristionchusbased on morphological observation and molecular phylogenetic analysis.

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