scholarly journals Interferon-γ Inhibits STAT6 Signal Transduction and Gene Expression in Human Airway Epithelial Cells

2004 ◽  
Vol 31 (5) ◽  
pp. 573-582 ◽  
Author(s):  
Nicola M. Heller ◽  
Satoshi Matsukura ◽  
Steve N. Georas ◽  
Mark R. Boothby ◽  
Paul B. Rothman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Epithelial Cells ◽  
Interferon Γ ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nadzeya Marozkina ◽  
Laura Smith ◽  
Yi Zhao ◽  
Joe Zein ◽  
James F. Chmiel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Nitrogen Oxides ◽  
Airway Epithelium ◽  
Airflow Obstruction ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Human Airway Epithelial Cells

AbstractEndothelial hemoglobin (Hb)α regulates endothelial nitric oxide synthase (eNOS) biochemistry. We hypothesized that Hb could also be expressed and biochemically active in the ciliated human airway epithelium. Primary human airway epithelial cells, cultured at air–liquid interface (ALI), were obtained by clinical airway brushings or from explanted lungs. Human airway Hb mRNA data were from publically available databases; or from RT-PCR. Hb proteins were identified by immunoprecipitation, immunoblot, immunohistochemistry, immunofluorescence and liquid chromatography- mass spectrometry. Viral vectors were used to alter Hbβ expression. Heme and nitrogen oxides were measured colorimetrically. Hb mRNA was expressed in human ciliated epithelial cells. Heme proteins (Hbα, β, and δ) were detected in ALI cultures by several methods. Higher levels of airway epithelial Hbβ gene expression were associated with lower FEV1 in asthma. Both Hbβ knockdown and overexpression affected cell morphology. Hbβ and eNOS were apically colocalized. Binding heme with CO decreased extracellular accumulation of nitrogen oxides. Human airway epithelial cells express Hb. Higher levels of Hbβ gene expression were associated with airflow obstruction. Hbβ and eNOS were colocalized in ciliated cells, and heme affected oxidation of the NOS product. Epithelial Hb expression may be relevant to human airways diseases.

AP2α is essential for MUC8 gene expression in human airway epithelial cells

2010 ◽  
Vol 110 (6) ◽  
pp. 1386-1398 ◽  
Author(s):  
Uk Yeol Moon ◽  
Chang-Hoon Kim ◽  
Jae Young Choi ◽  
Yoon-Ju Kim ◽  
Yeon Ho Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Regulation of Thioredoxin Gene Expression by Vitamin A in Human Airway Epithelial Cells

2002 ◽  
Vol 26 (5) ◽  
pp. 627-635 ◽  
Author(s):  
Wen-Hsing Chang ◽  
Sekhar P.-M. Reddy ◽  
Yuan-Pu Peter Di ◽  
Ken Yoneda ◽  
Richart Harper ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Vitamin A ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

Patterns of Gene Expression in Human Airway Epithelial Cells

CHEST Journal ◽  
1997 ◽  
Vol 111 (6) ◽  
pp. 83S ◽  
Author(s):  
James C. Willey ◽  
Mark W. Frampton ◽  
Mark J. Utell ◽  
Michael J. Apostolakos ◽  
Erin L. Coy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Expression of the endocannabinoid system in the human airway epithelial cells – Impact of sex and chronic respiratory disease status

2020 ◽  
Author(s):  
Matthew F. Fantauzzi ◽  
Jennifer A. Aguiar ◽  
Benjamin J-M. Tremblay ◽  
Toyoshi Yanagihara ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Endocannabinoid System ◽  
Disease Status ◽  
Chronic Respiratory Disease ◽  
Airway Epithelial Cells ◽  
Smoke Inhalation ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

ABSTRACTRecreational and medicinal cannabis consumption in the past 12 months has been reported in 1/5th of Canadians, with greater use in males relative to females. Cannabis smoking is the dominant route of delivery in consumers, with the airway epithelium functioning as the site of first contact for inhaled phytocannabinoids. The endocannabinoid system is responsible for mediating the physiological effects of inhaled phytocannabinoids. Acute cannabis smoke inhalation can result in bronchodilation, which may have applications in chronic respiratory disease management. In contrast, chronic cannabis smoke inhalation is associated with reduced lung function and bronchitis, which challenges potential applications in the lung. The contribution of the endocannabinoid system in the airway epithelium to either beneficial or harmful physiological responses remains to be clearly defined in males and females and those with underlying chronic respiratory disease.To begin to address this knowledge gap, a curated dataset of 1090 unique human bronchial brushing gene expression profiles was created from Gene Expression Omnibus deposited microarray datasets. The dataset included 616 healthy subjects, 136 subjects with asthma, and 338 subjects with COPD. A 27-gene endocannabinoid signature was analyzed across all samples with sex and disease specific-analyses performed. Immunohistochemistry and immunoblots were performed to confirm in situ and in vitro protein expression of select genes in human airway epithelial cells.We confirm three receptors for cannabinoids, CB1, CB2, and TRPV1, are expressed at the protein level in human airway epithelial cells in situ and in vitro, justifying examining the downstream endocannabinoid pathway more extensively at the gene expression level. Sex status was associated with differential expression of 6/27 genes. In contrast, disease status was associated with differential expression of 18/27 genes in asthmatics and 22/27 genes in COPD subjects. We confirm at the protein level that TRPV1, the most differentially expressed candidate in our analyses, was up-regulated in airway epithelial cells from asthmatics relative to healthy subjects.Our data demonstrate that endocannabinoid system is expressed in human airway epithelial cells with expression impacted by disease status and minimally by sex. The data suggest that cannabis consumers may have differential physiological responses in the respiratory mucosa, which could impact both acute and chronic effects of cannabis smoke inhalation.

scholarly journals Effect of Berberine on MUC5AC Mucin Gene Expression and Mucin Production from Human Airway Epithelial Cells

2011 ◽  
Vol 19 (3) ◽  
pp. 320-323 ◽  
Author(s):  
Md. Asaduzzaman Sikder ◽  
Hyun-Jae Lee ◽  
Su-Yel Lee ◽  
Heung-Seog Bae ◽  
Jang-Hyun Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Mucin Production ◽  
Human Airway ◽  
Mucin Gene ◽  
Human Airway Epithelial Cells

Resistin upregulates MUC5AC/B mucin gene expression in human airway epithelial cells

2018 ◽  
Vol 499 (3) ◽  
pp. 655-661 ◽  
Author(s):  
Soyoung Kwak ◽  
Yong-Dae Kim ◽  
Hyung Gyun Na ◽  
Chang Hoon Bae ◽  
Si-Youn Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Mucin Gene ◽  
Human Airway Epithelial Cells
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