Drugs as Probes of Organ Function: Evaluation of the Hepatobiliary Axis Using Oral Rifampicin and Novel High Performance Liquid Chromatography

Investigation of the uptake and metabolism of drugs by organs such as the liver may allow assessment of specific aspects of organ function. Rifampicin, when orally administered, is transported into the hepatocyte from portal blood and thence passes, with its deacetylated metabolite, into the systemic circulation and into bile. This paper reports an investigation of the pharmacokinetics of a sub-therapeutic oral dose of rifampicin in healthy subjects, in patients with cirrhosis and in subjects with Gilbert's syndrome. The areas under the plasma concentration curves (AUC) in patients with cirrhosis were significantly greater than in healthy subjects. Subjects with Gilbert's syndrome had decreased AUCs compared with healthy subjects and were clearly distinguished from patients with cirrhosis. Rifampicin concentration in serum was measured by HPLC using a novel direct injection technique.

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Simple and rapid high-performance liquid chromatography analysis of propentofylline and its main metabolites in serum using a direct injection technique

Biomedical Chromatography ◽

10.1002/(sici)1099-0801(199908)13:5<340::aid-bmc883>3.0.co;2-v ◽

1999 ◽

Vol 13 (5) ◽

pp. 340-343 ◽

Cited By ~ 7

Author(s):

Naotaka Kuroda ◽

Yozo Hamachi ◽

Noriko Aoki ◽

Mitsuhiro Wada ◽

Mihoko Tanigawa ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Direct Injection ◽

Injection Technique ◽

Chromatography Analysis ◽

Performance Liquid Chromatography Analysis

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Abernethy Malformation in Combination with Gilbert’s Syndrome

Russian Journal of Gastroenterology Hepatology Coloproctology ◽

10.22416/1382-4376-2020-30-5-49-57 ◽

2020 ◽

Vol 30 (5) ◽

pp. 49-57

Author(s):

N. B. Gubergrits ◽

E. L. Bondar ◽

E. A. Dyadyk ◽

E. V. Berezhnaya ◽

Yu. E. Chirkov ◽

...

Keyword(s):

Portal Vein ◽

Vascular Anomaly ◽

Systemic Circulation ◽

Dynamic Monitoring ◽

Medical Attention ◽

Ultrasound Screening ◽

Abernethy Malformation ◽

Gilbert’S Syndrome ◽

Gilbert's Syndrome ◽

The Right

Aim. To present a clinical case of the Abernethy syndrome.Key points. Abernethy syndrome is a rare vascular anomaly associated with a congenital absence of the portal vein, as a result of which portal blood from the intestines and spleen drains directly into the systemic circulation bypassing the liver though a complete or partial shunt. In the vast majority of cases, Abernethy syndrome is manifested during the newborn period by jaundice syndrome, hypergalactosemia and encephalopathy. In rare cases, this vascular malformation is diagnosed in older patients during ultrasound screening. A 31 year-old patient sought medical attention with the complaints of sleep disturbance and fatigue. The conducted instrumental observation revealed echo-signs of malformation (agenesia) of the portal vein, which was further confirmed by both X-ray-contrast computed tomography and the pathohistological analysis of liver biopsy slides. The genotype UGT1A1•28 confirmed Gilbert's syndrome. Neutropenia (0.8 × 109/L) with a drop in the level of segmented neutrophils up to 27% was regarded as shunt neutropenia. Number connection test confirmed shunt encephalopathy. Conservative therapy for correcting hepatic encephalopathy was prescribed, followed by a dynamic monitoring of the patient’s condition.Conclusion. Diagnosis of Abernethy malformation is important for choosing the right treatment for the timely correction of complications of the disease and early detection of adenoma or hepatocellular carcinoma.

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