Plasma capric acid concentrations in healthy subjects determined by high-performance liquid chromatography

In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot using high performance liquid chromatography-photodiode array with lansoprazole as an internal standard. The analytical separation was performed on a C-18 column (Waters, Sunfire™ 5 μm; 250 × 4.6 mm) at 40 °C. The mobile phase used was acetonitrile–phosphate buffer pH 7.6 (40:60% v/v) with a flow rate of 1.00 mL/min; and was detected at 300 nm. The analyte was extracted from dried blood spot by methanol. Incurred sample stability was evaluated from 6 healthy subjects on day 0, 7, 14, and 28, respectively. This method was linear in the range concentration of 70–1400 ng/mL with r > 0.98. Pharmacokinetic study shows that the average of AUC0–t of EMP in the DBS sample was 1765.41 ngh/mL. The highest percent difference value of esomeprazole’s incurred samples stability on day 7, 14, and 28 from 6 healthy subjects were 9.81%. This result fulfilled the acceptance criteria, which is the percent difference should not be greater than 20%, and 67% of total samples have to fulfill the criteria. The incurred sample stability result showed that esomeprazole was stable in the DBS sample at least until 28 days with the highest value of percent difference is 9.81%.

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Genotyping of the Lactase-Phlorizin Hydrolase C/T-13910 Polymorphism by Means of a New Rapid Denaturing High-Performance Liquid Chromatography-Based Assay in Healthy Subjects and Colorectal Cancer Patients

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057107301328 ◽

2007 ◽

Vol 12 (5) ◽

pp. 733-739 ◽

Cited By ~ 8

Author(s):

Ada Piepoli ◽

Enrico Schirru ◽

Angela Mastrorilli ◽

Annamaria Gentile ◽

Rosa Cotugno ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Cancer Patients ◽

Healthy Subjects ◽

High Performance ◽

Genetic Variant ◽

Increased Risk ◽

Colorectal Cancer Patients

Adult-type hypolactasia results from the progressive decline of lactase-phlorizin hydrolase activity in enterocytes after weaning. Lactase nonpersistence may determine a primary lactose intolerance with reduced diary product consumption, which is possibly related to an increased risk of colon cancer. Recently, a genetic variant C/T—13910 upstream of the lactase-phlorizin hydrolase ( LCT) gene has been strongly correlated with the lactase persistence/nonpersistence trait in both family and case-control studies. The authors validate a denaturing high-performance liquid chromatography (dHPLC)—based assay versus conventional genotype sequencing in detecting the C/T—13910 polymorphism of LCT and evaluate its prevalence in 2 different Italian geographical areas and in colorectal cancer patients. DNA samples of 157 healthy subjects and 124 colon cancer patients from Apulia and of 97 healthy subjects from Sardinia were evaluated for the C/T—13910 polymorphism by dHPLC, sequencing, and restriction fragment length polymorphism (RFLP). Under optimized conditions, dHPLC was as sensitive as DNA sequencing and detected a new genetic variant (T/C-13913) in 2 individuals that was not identified by RFLP assay. Frequency of lactase nonpersistence genotype (C/C—13910) was similar in healthy subjects from 2 different Italian geographical areas and not increased in patients with colorectal cancer. The results indicate that the dHPLC method may be used as a rapid, noninvasive, and laborsaving screening tool for genotyping C/T—13910 polymorphism, with high success, low cost, and reproducibility. ( Journal of Biomolecular Screening 2007:733-739)

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Assay of Urinary Free Fucose by Fluorescence Labeling and High-Performance Liquid Chromatography

Clinical Chemistry ◽

10.1093/clinchem/38.5.752 ◽

1992 ◽

Vol 38 (5) ◽

pp. 752-755 ◽

Cited By ~ 11

Author(s):

J Suzuki ◽

A Kondo ◽

I Kato ◽

S Hase ◽

T Ikenaka

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Cancer Patients ◽

Healthy Subjects ◽

High Performance ◽

Fluorescence Labeling ◽

Anion Exchange Column ◽

Highly Sensitive ◽

Cleanup Procedure ◽

The Difference

Abstract The concentrations of free fucose and other sugars in urine of cancer patients and healthy subjects were analyzed by high-performance liquid chromatography. After the urine samples were dried, we coupled the sugars in the residue with 2-aminopyridine to be detected as fluorescent derivatives. We then analyzed the pyridylamino derivatives of the sugars with an anion-exchange column and borate buffer. The difference between cancer patients and healthy subjects for the mean concentrations of fucose corrected for creatinine was significant (P less than 0.001). We checked the relationship between the concentrations of other sugars and the presence of cancer. This method is highly sensitive, and neither a cleanup procedure before labeling nor purification before injection into the column is needed. Not only fucose but also other sugars can be detected simultaneously, so this method should be useful for studying any changes in sugars in urine in various diseases.

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An assay of uroporphyrinogen decarboxylase in erythrocytes.

Clinical Chemistry ◽

10.1093/clinchem/34.11.2355 ◽

1988 ◽

Vol 34 (11) ◽

pp. 2355-2357 ◽

Cited By ~ 18

Author(s):

J McManus ◽

D Blake ◽

S Ratnaike

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Fluorescence Detection ◽

Whole Blood ◽

Healthy Subjects ◽

Trichloroacetic Acid ◽

High Performance ◽

Porphyria Cutanea Tarda ◽

Internal Standard ◽

Uroporphyrinogen Decarboxylase

Abstract Measurement of uroporphyrinogen decarboxylase (UROD; EC 4.1.1.37) activity in erythrocytes is useful in distinguishing between familial porphyria cutanea tarda (PCT), in which UROD activities are low, and acquired PCT, in which UROD activity is normal. In this method for measuring UROD, pentacarboxylic acid porphyrinogen I (PPI) is used as substrate. A sample of the patient's whole blood is incubated with PPI at 37 degrees C for 30 min at pH 6.0. The reaction is stopped by adding trichloroacetic acid/dimethyl sulfoxide containing mesoporphyrin (internal standard). The coproporphyrin so produced is measured directly by high-performance liquid chromatography, with fluorescence detection. Our values by this method for healthy subjects and non-PCT patients ranged from 1.8 to 4.0 U/L. The CV for the assay was 10% at 1.1 U/L and 9% at 2.4 U/L. Twelve of 42 patients with PCT had low erythrocyte UROD activities. In each of six families of patients with low UROD activity we found at least one other family member with a low UROD activity in erythrocytes.

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Carbamylated Haemoglobin in Normal, Diabetic and Uraemic Patients

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329202900215 ◽

1992 ◽

Vol 29 (2) ◽

pp. 206-209 ◽

Cited By ~ 16

Author(s):

Jonathan T C Kwan ◽

Edwin C Carr ◽

Jeffrey L Barron ◽

Michael R Bending

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Healthy Subjects ◽

High Performance ◽

Similar Degree ◽

Dialysis Treatment ◽

Significant Difference ◽

Hospital Patients

Carbamylated haemoglobin arises from the non-enzymic modification of haemoglobin monomers by isocyanate derived from the spontaneous dissociation of urea. We measured carbamylated haemoglobin by high performance liquid chromatography in healthy subjects, non-uraemic hospital patients, diabetics, and different groups of uraemic patients. Carbamylated haemoglobin levels were found to be raised in uraemic subjects, but were independent of age, sex, glycaemic state and haemodialysis procedure. There was no significant difference in carbamylated haemoglobin levels between two groups of patients having different modes of dialysis treatment, probably indicating a similar degree of uraemic exposure in these patients.

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Quantification of gliclazide by semi-micro high-performance liquid chromatography: application to a bioequivalence study of two formulations in healthy subjects

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2004.02.025 ◽

2004 ◽

Vol 35 (4) ◽

pp. 943-949 ◽

Cited By ~ 19

Author(s):

Ji-Young Park ◽

Kyoung-Ah Kim ◽

Su-Lyun Kim ◽

Pil-Whan Park

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Healthy Subjects ◽

High Performance ◽

Bioequivalence Study

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PHARMACOKINETIC PROFILE OF METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOT OF HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.78 ◽

2018 ◽

Vol 10 (1) ◽

pp. 354

Author(s):

Yahdiana Harahap ◽

Citra Dara Malia ◽

Sunarsih .

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Healthy Subjects ◽

High Performance ◽

Pharmacokinetic Profile ◽

Dried Blood Spot ◽

Metformin Hydrochloride ◽

Linear Calibration ◽

Photodiode Array ◽

Blood Spot

Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in sixhealthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatography–photodiode array(HPLC–PDA).Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Bloodsamples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLC–PDA.Results: A linear calibration curve was obtained in the range of 25–5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtainedusing DBS samples from six healthy participants, with a Cmax of 347.3–416.22 ng/mL. The average tmax and t1/2 were obtained at 3 and 2 h, respectively,and the area under the curve (AUC)0-t/AUC0-∞ ratio was >80% for all subjects.Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.

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