Induction of Sister-chromatid Exchanges, Micronuclei and Gene Mutations by Indirectly Acting Promutagens Using Human Hepatoma Cells as an Activation System

1994 ◽  
Vol 22 (6) ◽  
pp. 445-453
Author(s):  
Firouz Darroudi ◽  
Adayapalam T. Natarajan
Keyword(s):  
Sister Chromatid ◽  
Cho Cells ◽  
Point Mutations ◽  
Biologically Active ◽  
Target Cells ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

An established human hepatoma cell strain (designated Hep G2) was used in sister-chromatid exchange (SCE) and micronuclei (MN) assays to study, in vitro, the genotoxic potentials of indirectly acting promutagenic carcinogens, such as 2-acetylaminofluorene (2-AAF) and hexamethylphosphoramide (HMPA), and a non-carcinogen, 4-acetylaminofluorene (4-AAF). In addition, Hep G2 S9-fractions were isolated and used to activate the same chemicals using Chinese hamster ovary (CHO) cells as target cells in vitro. The percentage survival and the frequencies of MN, SCEs and point mutations (at the HPRT locus) were used as biological endpoints. A dose-dependent increase in the frequencies of SCEs, MN, cytotoxicity, and/or point mutations was found in Hep G2 cells, and in CHO cells in the presence of Hep G2 S9-fractions, with 2-AAF and HMPA, but with 4-AAF no increase was found. The results obtained demonstrate that the Hep G2 cells and their S9-fractions are capable of activating different classes of mutagens to form biologically active metabolites.

The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies

1988 ◽  
Vol 16 (1) ◽  
pp. 16-22
Author(s):  
Marina Marinovich ◽  
Jose L. Lorenzo ◽  
Liliana M. Flaminio ◽  
Agnese Granata ◽  
Corrado L. Galli
Keyword(s):  
Cell Line ◽  
Rat Hepatocytes ◽  
Prostaglandin E ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Isolated Rat Hepatocytes ◽  
G2 Cells ◽  
Isolated Rat

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.

In vitro metabolic activation of promutagenic carcinogens with human hepatoma (Hep G2) cells

1996 ◽  
Vol 360 (3) ◽  
pp. 289
Author(s):  
F. Darroudi ◽  
C. Meijers ◽  
V. Hadjidekova ◽  
F.J. Kasper ◽  
A.T. Natarajan
Keyword(s):  
Metabolic Activation ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

Synthesis and Processing of Human Serum Apolipoprotein AII in vitro and in Hep G2 Cells

1985 ◽  
Vol 366 (1) ◽  
pp. 173-180 ◽  
Author(s):  
Wilhelm STOFFEL ◽  
Rosemarie BLAU ◽  
Martin BURK
Keyword(s):  
Human Serum ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Synthesis And Processing ◽  
G2 Cells

Overexpression of CYP27 in hepatic and extrahepatic cells: role in the regulation of cholesterol homeostasis

2001 ◽  
Vol 281 (1) ◽  
pp. G293-G301 ◽  
Author(s):  
E. Hall ◽  
P. Hylemon ◽  
Z. Vlahcevic ◽  
D. Mallonee ◽  
K. Valerie ◽  
...  
Keyword(s):  
Bile Acid ◽  
Cho Cells ◽  
Specific Activity ◽  
Recombinant Adenovirus ◽  
Cholesterol Acyltransferase ◽  
Chinese Hamster ◽  
Cholesterol Homeostasis ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

In the liver, sterol 27-hydroxylase (CYP27) participates in the classic and alternative pathways of bile acid biosynthesis from cholesterol (Chol). In extrahepatic tissues, CYP27 converts intracellular Chol to 27-hydroxycholesterol (27OH-Chol), which may regulate the activity of 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoA-R). This study attempts to better define the role of CYP27 in the maintenance of Chol homeostasis in hepatic and extrahepatic cells by overexpressing CYP27 in Hep G2 cells and Chinese hamster ovary (CHO) cells through infection with a replication-defective recombinant adenovirus encoding for CMV-CYP27. After infection, CYP27 mRNA and protein levels increased dramatically. CYP27 specific activity also increased two- to fourfold in infected cells ( P ≤ 0.02), with a marked increase in conversion of [14C]Chol to [14C]27OH-Chol (∼150%; P ≤ 0.01). Accumulation of 27OH-Chol in CHO cells was associated with a 50% decrease in HMG-CoA-R specific activity ( P ≤ 0.02). In infected Hep G2 cells, the significant increase in bile acid synthesis (46%; P ≤ 0.006), which prevented the accumulation of intracellular 27OH-Chol, resulted in increased HMG-CoA-R activity (183%; P ≤ 0.02). Overexpression of CYP27 in Hep G2 cells also increased acyl CoA-cholesterol acyltransferase (71%, P ≤ 0.02) and decreased cholesteryl ester hydrolase (55%, P ≤ 0.02). In conclusion, CYP27 generates different physiological responses depending on cell type and presence or absence of bile acid biosynthetic pathways.

Modelling ethanol cytotoxicity in vitro, in Hep G2 cells

1994 ◽  
Vol 27 (3) ◽  
pp. 211
Author(s):  
M.G. Neuman ◽  
N.H. Shear ◽  
C. Tiribelli
Keyword(s):  
Hep G2 ◽  
Hep G2 Cells ◽  
Ethanol Cytotoxicity ◽  
G2 Cells

scholarly journals Fukinolic Acid Derivatives and Triterpene Glycosides from Black Cohosh Inhibit CYP Isozymes, but are Not Cytotoxic to Hep-G2 Cells In Vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 118-124 ◽  
Author(s):  
Yue Huang ◽  
Bei Jiang ◽  
Paiboon Nuntanakorn ◽  
Edward Kennelly ◽  
Stacy Shord ◽  
...  
Keyword(s):  
Triterpene Glycosides ◽  
Black Cohosh ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Cyp Isozymes

P XIV A.5 Metabolic activation of promutagenic carcinogens with human hepatoma (Hep G2) cells

1997 ◽  
Vol 379 (1) ◽  
pp. S124
Author(s):  
F. Darroudi ◽  
S. Knasmüller ◽  
R. Sanyal ◽  
C.M. Meijers ◽  
A.T. Natarajan
Keyword(s):  
Metabolic Activation ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells
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