The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies

1988 ◽  
Vol 16 (1) ◽  
pp. 16-22
Author(s):  
Marina Marinovich ◽  
Jose L. Lorenzo ◽  
Liliana M. Flaminio ◽  
Agnese Granata ◽  
Corrado L. Galli
Keyword(s):  
Cell Line ◽  
Rat Hepatocytes ◽  
Prostaglandin E ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Isolated Rat Hepatocytes ◽  
G2 Cells ◽  
Isolated Rat

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.

scholarly journals The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2

1983 ◽  
Vol 214 (3) ◽  
pp. 951-958 ◽  
Author(s):  
L Havekes ◽  
V van Hinsbergh ◽  
H J Kempen ◽  
J Emeis
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
High Affinity ◽  
G2 Cells

The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a KD of 25 nM-LDL and a maximal amount of binding of about 70 ng of LDL-apoprotein/mg of cell protein. The high-affinity binding, uptake and degradation of LDL by Hep G2 cells is dependent on the extracellular Ca2+ concentration and is down-regulated by the presence of fairly high concentrations of extracellular LDL. Incubation of the Hep G2 cells with LDL results in suppression of the intracellular cholesterol synthesis. It is concluded that the human hepatoma cell line Hep G2 possesses specific LDL receptors similar to the LDL receptors demonstrated on extrahepatic tissue cells.

In vitro metabolic activation of promutagenic carcinogens with human hepatoma (Hep G2) cells

1996 ◽  
Vol 360 (3) ◽  
pp. 289
Author(s):  
F. Darroudi ◽  
C. Meijers ◽  
V. Hadjidekova ◽  
F.J. Kasper ◽  
A.T. Natarajan
Keyword(s):  
Metabolic Activation ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

Induction of Sister-chromatid Exchanges, Micronuclei and Gene Mutations by Indirectly Acting Promutagens Using Human Hepatoma Cells as an Activation System

1994 ◽  
Vol 22 (6) ◽  
pp. 445-453
Author(s):  
Firouz Darroudi ◽  
Adayapalam T. Natarajan
Keyword(s):  
Sister Chromatid ◽  
Cho Cells ◽  
Point Mutations ◽  
Biologically Active ◽  
Target Cells ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

An established human hepatoma cell strain (designated Hep G2) was used in sister-chromatid exchange (SCE) and micronuclei (MN) assays to study, in vitro, the genotoxic potentials of indirectly acting promutagenic carcinogens, such as 2-acetylaminofluorene (2-AAF) and hexamethylphosphoramide (HMPA), and a non-carcinogen, 4-acetylaminofluorene (4-AAF). In addition, Hep G2 S9-fractions were isolated and used to activate the same chemicals using Chinese hamster ovary (CHO) cells as target cells in vitro. The percentage survival and the frequencies of MN, SCEs and point mutations (at the HPRT locus) were used as biological endpoints. A dose-dependent increase in the frequencies of SCEs, MN, cytotoxicity, and/or point mutations was found in Hep G2 cells, and in CHO cells in the presence of Hep G2 S9-fractions, with 2-AAF and HMPA, but with 4-AAF no increase was found. The results obtained demonstrate that the Hep G2 cells and their S9-fractions are capable of activating different classes of mutagens to form biologically active metabolites.

Acellular liver matrix improves the survival and functions of isolated rat hepatocytes cultured in vitro.

2004 ◽  
Author(s):  
Patrizia Burra ◽  
Silvia Tomat ◽  
Maria Teresa Conconi ◽  
Carlo Macchi ◽  
Francesco P Russo ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Preparation of microgram quantities of BaP-DNA adducts using isolated rat hepatocytes in vitro

1989 ◽  
Vol 10 (4) ◽  
pp. 789-791 ◽  
Author(s):  
David A. Dankovic ◽  
David L. Springer ◽  
David B. Mann ◽  
Linda G. Smith ◽  
Berta L. Thomas ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat
Sign in / Sign up

Export Citation Format

Share Document