scholarly journals MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome

2019 ◽  
Vol 47 (9) ◽  
pp. 4163-4182
Author(s):  
Jin Hou ◽  
Lei Zhao ◽  
Jing Yan ◽  
Xiaoyong Ren ◽  
Kang Zhu ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Expression Profiles ◽  
Upper Airway ◽  
Target Prediction ◽  
Mirna Array ◽  
Real Time Quantitative Pcr ◽  
Reverse Transcription Pcr ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome

Objective To investigate the involvement of microRNAs (miRNAs) in the pathogenesis of obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods In this study, we investigated miRNA profiles in the upper airway (UA) skeletal muscles of four patients with OSAHS and four matched controls using the miRCURY miRNA array. In another cohort of 12 OSAHS cases and 7 controls, the mRNA expression levels of interleukin (IL)-6 and Lin-28 homolog A (Lin28A), targets of the downregulated let-7 family members, were measured by real-time quantitative-PCR. The potential targets of the miRNAs were predicted by miRNA target prediction databases miRanda, Microcosm, and Targetscan. Results The array identified 370 differentially expressed miRNAs, of which 181 were upregulated and 189 were downregulated in OSAHS patients (based on a fold-change >2.0 and p < 0.05). Upregulation of IL-6 and Lin28A was validated by quantitative reverse transcription PCR. The 612 targets predicted by all three algorithms were subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The results revealed perturbations in signaling pathways and cellular functions. Conclusion This study demonstrated profoundly altered miRNA expression profiles in upper airway muscular tissues of patients with OSAHS, which might contribute to the formation and development of OSAHS.

Relationship between the Upper Airway and Obstructive Sleep Apnea-Hypopnea Syndrome in Morbidly Obese Women

2007 ◽  
Vol 17 (5) ◽  
pp. 689-697 ◽  
Author(s):  
A. Santiago-Recuerda ◽  
F. J. Gómez-Terreros ◽  
P. Caballero ◽  
A. Martín-Duce ◽  
M. J. Soleto ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Upper Airway ◽  
Morbidly Obese ◽  
Obese Women ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome

320-Detector CT Imaging of the Upper Airway Structure of Patients With Obstructive Sleep Apnea-Hypopnea Syndrome

2012 ◽  
Vol 23 (3) ◽  
pp. 675-677 ◽  
Author(s):  
Binbin Ying ◽  
Qiuli Huang ◽  
Yingsheng Su ◽  
Bonian Fu ◽  
Xianwang Ye ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Upper Airway ◽  
Ct Imaging ◽  
Obstructive Sleep ◽  
Upper Airway Structure ◽  
Hypopnea Syndrome

scholarly journals Morphologic Analyses of Mandible and Upper Airway Soft Tissue by MRI of Patients With Obstructive Sleep Apnea Hypopnea Syndrome

SLEEP ◽  
2006 ◽  
Vol 29 (7) ◽  
pp. 909-915 ◽  
Author(s):  
Mau Okubo ◽  
Masaaki Suzuki ◽  
Atsushi Horiuchi ◽  
Shinichi Okabe ◽  
Katsuhisa Ikeda ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Soft Tissue ◽  
Upper Airway ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome

scholarly journals The Finite Element Simulation of the Upper Airway of Patients with Moderate and Severe Obstructive Sleep Apnea Hypopnea Syndrome

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Huiping Luo ◽  
Austin Scholp ◽  
Jack J. Jiang
Keyword(s):  
Finite Element ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Soft Tissue ◽  
Soft Tissues ◽  
Upper Airway ◽  
Model Parameters ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome ◽  
Fluid Solid Interaction

Objectives. To investigate the snoring modes of patients with Obstructive Sleep Apnea Hypopnea Syndrome and to discover the main sources of snoring in soft tissue vibrations. Methods. A three-dimensional finite element model was developed with SolidEdge to simulate the human upper airway. The inherent modal simulation was conducted to obtain the frequencies and the corresponding shapes of the soft tissue vibrations. The respiration process was simulated with the fluid-solid interaction method through ANSYS. Results. The first 6 orders of modal vibration were 12 Hz, 18 Hz, 21 Hz, 22 Hz, 36 Hz, and 39 Hz. Frequencies of modes 1, 2, 4, and 5 were from tongue vibrations. Frequencies of modes 3 and 6 were from soft palate vibrations. Steady pressure distribution and air distribution lines in the upper airway were shown clearly in the fluid-solid interaction simulation results. Conclusions. We were able to observe the vibrations of soft tissue and the modeled airflow by applying the finite element methods. Future studies could focus on improving the soft tissues vibration compliances by adjusting the model parameters. Additionally, more attention should be paid to vibrational components below 20 Hz when performing an acoustic analysis of human snore sounds due to the presence of these frequencies in this model.

scholarly journals Surface Tension of Upper Airway Mucosal Lining Liquid in Obstructive Sleep Apnea/Hypopnea Syndrome

SLEEP ◽  
2005 ◽  
Vol 28 (4) ◽  
pp. 457-463 ◽  
Author(s):  
Jason P. Kirkness ◽  
Melanie Madronio ◽  
Rosie Stavrinou ◽  
John R. Wheatley ◽  
Terence C. Amis
Keyword(s):  
Surface Tension ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Upper Airway ◽  
Obstructive Sleep ◽  
Mucosal Lining ◽  
Hypopnea Syndrome

Collapsible upper airway segment to study the obstructive sleep apnea/hypopnea syndrome in rats

2003 ◽  
Vol 136 (2-3) ◽  
pp. 199-209 ◽  
Author(s):  
Ramon Farré ◽  
Mar Rotger ◽  
Josep M. Montserrat ◽  
Gabriel Calero ◽  
Daniel Navajas
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Upper Airway ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome

Relationship between surface tension of upper airway lining liquid and upper airway collapsibility during sleep in obstructive sleep apnea hypopnea syndrome

2003 ◽  
Vol 95 (5) ◽  
pp. 1761-1766 ◽  
Author(s):  
Jason P. Kirkness ◽  
Melanie Madronio ◽  
Rosie Stavrinou ◽  
John R. Wheatley ◽  
Terence C. Amis
Keyword(s):  
Surface Tension ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Sleep Disordered Breathing ◽  
Upper Airway ◽  
Obstructive Sleep ◽  
Before And After ◽  
Posterior Pharynx ◽  
Hypopnea Syndrome ◽  
Disordered Breathing

Lowering surface tension (γ) of upper airway lining liquid (UAL) reduces upper airway opening (anesthetized humans) and closing (anesthetized rabbits) pressures. We now hypothesize that in sleeping obstructive sleep apnea hypopnea syndrome (OSAHS) patients lowering γ of UAL will enhance upper airway stability and decrease the severity of sleep-disordered breathing. Nine OSAHS patients [respiratory disturbance index (RDI): 49 ± 8 (SE) events/h, diagnostic night] participated in a two-part, one-night, polysomnography study. In the first part, upper airway closing pressures (during non-rapid eye movement sleep, Pcrit) were measured and samples of UAL (awake) were obtained before and after 2.5 ml of surfactant (Exosurf, Glaxo Smith Kline) was instilled into the posterior pharynx. The γ of UAL was determined with the use of the “pull-off” force technique. In the second part, subjects received a second application of 2.5 ml of surfactant and then slept the remainder of the night (205 ± 30 min). Instillation of surfactant decreased the γ of UAL from 60.9 ± 3.1 mN/m (control) to 45.2 ± 2.5 mN/m (surfactant group) ( n = 9, P < 0.001). Pcrit decreased from 1.19 ± 1.14 cmH2O (control) to -0.56 ± 1.15 cmH2O (surfactant group) ( n = 7, P < 0.02). Compared with the second half of diagnostic night, surfactant decreased RDI from 51 ± 8 to 35 ± 8 events/h ( n = 9, P < 0.03). The fall in RDI (ΔRDI) correlated with the fall in γ of UAL (Δγ) (ΔRDI = 1.8 × Δγ, r = 0.68, P = 0.04). Hypopneas decreased ∼50% from 42 ± 8 to 20 ± 5 events/h ( n = 9, P < 0.03, paired t-test). The γ of UAL measured the next morning remained low at 49.5 ± 2.7 mN/m ( n = 9, P < 0.001, ANOVA, compared with control). In conclusion, instillation of surfactant reduced the γ of UAL in OSAHS patients and decreased Pcrit and the occurrence of hypopneas. Therapeutic manipulation of γ of UAL may be beneficial in reducing the severity of sleep-disordered breathing in OSAHS patients.

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