scholarly journals Presumed Primary Muscular Lymphoma in a Dog

2008 ◽  
Vol 20 (6) ◽  
pp. 824-826 ◽  
Author(s):  
Céline Thuilliez ◽  
Dorothée Watrelot-Virieux ◽  
Franck Chanut ◽  
Corinne Fournel-Fleury ◽  
Frédérique Ponce ◽  
...  
Keyword(s):  
Anaplastic Large Cell Lymphoma ◽  
Cell Lymphoma ◽  
Large Cell ◽  
Needle Aspiration ◽  
Neoplastic Cell ◽  
Intact Male ◽  
Cell Reactivity ◽  
Ki 67 ◽  
Large Cell Lymphoma ◽  
Cluster Of Differentiation

A case of presumed primary muscular lymphoma in an 8-year-old, intact, male Newfoundland dog is reported. The dog was presented for evaluation of an infiltrating ventral cervical mass, respiratory distress, and anorexia of 1-month duration. Fine-needle aspiration of the mass revealed anaplastic large cell lymphoma. Despite chemotherapy, health status declined and the animal was euthanized a few weeks later. At necropsy, the mass infiltrated the cervical muscles and extended ventrally to the left forelimb and cranially to the tongue and laryngeal musculature. Other muscles were infiltrated by the same neoplasm (diaphragm and intercostal, abdominal, and gluteal muscles) indicating a probable multicentric origin. Histological examination confirmed the diagnosis of anaplastic large cell lymphoma, which showed a strong muscular tropism. Immunohistochemical staining revealed neoplastic cell reactivity for cluster of differentiation 3 (CD3) and Ki-67 antigens (70% and 90%, respectively). The neoplastic cells were negative for CD79a. The presumed histological diagnosis in this dog was primary muscular anaplastic large T-cell lymphoma.

Fine-needle aspiration biopsy of ki-1-positive anaplastic large-cell lymphoma

1992 ◽  
Vol 8 (3) ◽  
pp. 242-247 ◽  
Author(s):  
Mohammed Akhtar ◽  
Muhammad Ashraf Ali ◽  
Abdulrazzaq Haider ◽  
John Antonius ◽  
Bo Hainau ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Anaplastic Large Cell Lymphoma ◽  
Fine Needle Aspiration Biopsy ◽  
Cell Lymphoma ◽  
Large Cell ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Large Cell Lymphoma ◽  
Needle Aspiration Biopsy

scholarly journals Anaplastic lymphoma kinase immunocytochemistry in fine needle aspiration diagnosis of anaplastic large-cell lymphoma

2018 ◽  
Vol 35 (1) ◽  
pp. 37
Author(s):  
Saumyaranjan Mallick ◽  
Prashant Ramteke ◽  
Sanjeev Chitragar ◽  
Ashok Singh ◽  
SandeepR Mathur ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Anaplastic Large Cell Lymphoma ◽  
Anaplastic Lymphoma Kinase ◽  
Cell Lymphoma ◽  
Large Cell ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Anaplastic Lymphoma ◽  
Large Cell Lymphoma

Flow Cytometric Immunophenotyping of Anaplastic Large Cell Lymphoma

2009 ◽  
Vol 133 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Tariq Muzzafar ◽  
Eric X. Wei ◽  
Pei Lin ◽  
L. Jeffrey Medeiros ◽  
Jeffrey L. Jorgensen
Keyword(s):  
Flow Cytometry ◽  
Anaplastic Large Cell Lymphoma ◽  
Cell Lymphoma ◽  
Antigen Expression ◽  
Large Cell ◽  
Needle Aspiration ◽  
Neoplastic Cells ◽  
Flow Cytometric Immunophenotyping ◽  
Flow Cytometric ◽  
Large Cell Lymphoma

Abstract Context.—Anaplastic large cell lymphoma (ALCL) is usually diagnosed by histologic and immunohistochemical analysis. However, fine-needle aspiration is becoming a popular alternative to lymph node biopsy, and flow-cytometric immunophenotyping is often used to analyze fine-needle aspiration specimens. Objective.—To review our experience using flow-cytometric immunophenotyping to assess cases of ALCL and to evaluate the diagnostic utility of this technique. Design.—Each case of ALCL was assessed by flow cytometry with 3-color or 4-color antibody panels, and data were reanalyzed by cluster analysis using Paint-a-Gate for cases with retrievable flow cytometry data files. Anaplastic lymphoma kinase (ALK) was assessed by using immunohistochemistry. Results.—Twenty-three ALCL cases were analyzed by flow cytometry. In 4 cases, neoplastic cells could not be identified. In the remaining 19 cases (11 ALK+, 8 ALK−), all were positive for CD30 and CD45. Anaplastic large cell lymphoma cells were large and usually CD45 bright, with many or most cells falling in the region of monocytes on the CD45/side scatter plot. The frequencies of T-cell antigen expression in ALK+ cases were CD2, 67%; CD7, 60%; CD3, 45%; CD4, 33%; CD5, 14%; and CD8, 14%. In ALK− cases, the frequencies of the T-cell antigen expression were CD2, 100%; CD3, 50%; CD4, 40%; CD7, 40%; CD5, 25%; and CD8, 20%. Conclusions.—Flow cytometry can be used to immunophenotype ALCL cases. Neoplastic cells may be few, and they may fall outside of the lymphocyte gate. Cluster analysis using software like Paint-A-Gate is often helpful because it allows for flexible, sequential gating strategies to identify and characterize the neoplastic cells.

scholarly journals Gingival Anaplastic Large-Cell Lymphoma Mimicking Hyperplastic Benignancy as the First Clinical Manifestation of AIDS: A Case Report and Review of the Literature

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Rafaela Elvira Rozza-de-Menezes ◽  
Stefânia Jeronimo Ferreira ◽  
Diogo Lenzi Capella ◽  
Stephan Schwartz ◽  
Ana Helena Willrich ◽  
...  
Keyword(s):  
Clinical Manifestation ◽  
Anaplastic Large Cell Lymphoma ◽  
Cell Lymphoma ◽  
Excisional Biopsy ◽  
Large Cell ◽  
Final Diagnosis ◽  
Ki 67 ◽  
First Case ◽  
Abundant Cytoplasm ◽  
Large Cell Lymphoma

This paper presents an unusual case of gingival ALCL, which mimicked a benign hyperplastic lesion that occurred in a 57-year-old white man representing the first clinical manifestation of acquired immunodeficiency syndrome (AIDS). The patient was referred to the Dental Clinic of PUCPR complaining of a lobulated nodule on the gingiva of his upper central incisors. The presence of advanced chronic periodontitis and dental plaque raised suspicion for a benignancy. An excisional biopsy was performed, and large pleomorphic cells with an abundant cytoplasm, sometimes containing prominent nucleoli and “Hallmark” cells, were observed through hematoxylin and eosin staining. The tumor cells showed strong CD30 expression, EMA, Ki-67, and LCA, and negative stain forp80NPM/ALK, CKAE1/AE3, CD20, CD3, CD56, and CD15. The final diagnosis was ALCL (ALK-negative). Further laboratory tests revealed positivity for human immunodeficiency virus (HIV). The patient was submitted to chemotherapy, but four months after diagnosis, the patient died due to pneumonia and respiratory failure. Oral anaplastic large-cell lymphoma (ALCL) is a rare disorder. Only 5 cases involving the gingiva have been reported, and to our knowledge, this is the first case reported of the ALCL, which mimicked a hyperplastic benignancy as the first clinical manifestation of AIDS.

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