scholarly journals An efficient fiber-based ELISA for detection of antibody against fowl adenovirus serotypes 7 and 8

2020 ◽  
Vol 32 (3) ◽  
pp. 444-449
Author(s):  
Hao Lu ◽  
Weikang Wang ◽  
Jianjun Zhang ◽  
Hongxia Shao ◽  
Luyuan Li ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Immunofluorescence Assay ◽  
The Other ◽  
Economic Losses ◽  
Adenovirus Serotype ◽  
Coating Antigen ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis ◽  
Test Kit ◽  
Serology Test

An outbreak of inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8) has caused significant economic losses in the poultry industry worldwide. However, a rapid serology test kit specific to FAdV-8 is not available to date. We developed a fiber-based ELISA using the purified GST-fiber of FAdV-8 as coating antigen to measure antibodies against FAdV-8. Specificity analysis showed that our ELISA could react with sera against FAdV-7, -8a, and -8b, but not with sera against the other pathogens tested. Moreover, detection of positive sera with our ELISA had 83% and 94% agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek, respectively. Our ELISA was also effective in the detection of antibodies against FAdV-8 in sera from both experimentally infected and clinically vaccinated chickens. Our FAdV-8 fiber-based ELISA can be a valuable tool to specifically and sensitively detect antibodies against FAdV-7 and/or -8 in infected or vaccinated chickens.

scholarly journals Complete Genome Sequence of a Fowl Adenovirus D Strain Isolated from Chickens with Inclusion Body Hepatitis in Japan

2021 ◽  
Vol 10 (46) ◽  
Author(s):  
Masaji Mase ◽  
Hiroshi Iseki ◽  
Satoko Watanabe
Keyword(s):  
Inclusion Body ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Common Origin ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis

We report the complete genome sequence of fowl adenovirus D (FAdV-D) strain JP/Tokushima/2010IBH, which was isolated from chickens with inclusion body hepatitis in Japan. This FAdV-D isolate was genetically highly similar to recent isolates from China, suggesting a common origin.

Pathotypic and Molecular Characterization of a Fowl Adenovirus Associated with Inclusion Body Hepatitis in Saskatchewan Chickens

2012 ◽  
Vol 56 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Arshud Dar ◽  
Susantha Gomis ◽  
Ian Shirley ◽  
George Mutwiri ◽  
Robert Brownlie ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Inclusion Body ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis

scholarly journals Inclusion body hepatitis (IBH) outbreak associated with fowl adenovirus type 8b in broilers

2013 ◽  
Vol 63 (1) ◽  
pp. 101-110 ◽  
Author(s):  
M. Zadravec ◽  
Brigita Slavec ◽  
U. Krapez ◽  
Gl. Kaján ◽  
J. Racnik ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Adenovirus Type ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis

Inactivated and live bivalent fowl adenovirus (FAdV8b + FAdV11) breeder vaccines provide broad-spectrum protection in chicks against inclusion body hepatitis (IBH)

Vaccine ◽  
2018 ◽  
Vol 36 (5) ◽  
pp. 744-750 ◽  
Author(s):  
Ashish Gupta ◽  
Shelly Popowich ◽  
Davor Ojkic ◽  
Shanika Kurukulasuriya ◽  
Betty Chow-Lockerbie ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Broad Spectrum ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis

scholarly journals Fowl adenovirus Group I as a causal agent of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS) outbreak in brazilian broiler flocks

2014 ◽  
Vol 34 (8) ◽  
pp. 733-737 ◽  
Author(s):  
Elena Mettifogo ◽  
Luis F.N. Nuñez ◽  
Silvana H. Santander Parra ◽  
Claudete S. Astolfi-Ferreira ◽  
Antonio J. Piantino Ferreira
Keyword(s):  
Inclusion Body ◽  
Causal Agent ◽  
Hexon Gene ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Group I ◽  
Inclusion Body Hepatitis ◽  
Commercial Broiler ◽  
Gross Lesions ◽  
Poor Weight Gain

Commercial broiler flocks from a farm located in the State of São Paulo, Brazil, presented diarrhea, depression, increased mortality and poor weight gain. Upon post-mortem examination, classical signs of Inclusion Body Hepatitis/Hydropericardium Syndrome (IBH/HPS) were observed, including enlarged pale yellow-colored livers and straw-colored liquid in the pericardial sac. In addition, gross lesions were also observed in the kidneys, pancreas, thymus, intestines and gallbladder. Samples of these organs were analyzed by PCR for the detection of the hexon gene of the Fowl Adenovirus (FAdVs) Group I. The results were positive for both flocks (A and B) assayed by PCR. The macroscopic lesions associated with the detection of FAdV Group I by PCR in several of these affected organs allowed for the identification of IBH/HPS. In fact, this is the first report in Brazil of IBH/HPS in broilers, which identifies FAdVs group I as a causal agent of the disease. These findings may contribute to the worldwide epidemiology of the adenovirus-mediated hepatitis/hydropericardium syndrome.

Inclusion Body Hepatitis Caused by Fowl Adenovirus in Broiler Chickens in Japan, 2009–2010

2011 ◽  
Vol 55 (4) ◽  
pp. 719-723 ◽  
Author(s):  
Kikuyasu Nakamura ◽  
Masaji Mase ◽  
Yu Yamamoto ◽  
Katsutoshi Takizawa ◽  
Masahiko Kabeya ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Broiler Chickens ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis
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