To compare corneal confocal microscopy of eyes with Fuchs’ endothelial corneal dystrophy after two different endothelial surgeries

2021 ◽  
pp. 112067212110094
Author(s):  
Domenico Schiano-Lomoriello ◽  
Irene Abicca ◽  
Valeria Bono ◽  
Daniela Giannini ◽  
Rossella Annamaria Colabelli-Gisoldi ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Visual Outcome ◽  
Corneal Dystrophy ◽  
Curve Analysis ◽  
Corneal Oedema ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Corneal Confocal Microscopy ◽  
Corrected Distance Visual Acuity

Purpose: To study the features of corneal confocal microscopy of eyes with Fuchs’ endothelial corneal dystrophy (FECD) after successful Descemet stripping automated endothelial keratoplasty (DSAEK) versus Descemet membrane endothelial keratoplasty (DMEK). Methods: Thirty-two eyes affected by FECD with corneal oedema requiring a corneal graft were treated with DSAEK (15 eyes) or DMEK (17 eyes). All patients underwent in vivo corneal confocal microscopy (IVCCM) at 6 months postoperatively. We evaluated preoperative and postoperative corrected distance visual acuity (CDVA) and the correlation with IVCCM characteristics. Results: Using IVCCM, Z-scan curve analysis showed similar subepithelial reflectivity peaks between the two groups (DSAEK 1256 SU ± 514 vs DMEK 1118 SU ± 408, p = 0.411), while the interface reflectivity was significantly higher in the DMEK group (1511 SU ± 357) than in the DSAEK group (1029 SU ± 413, p = 0.002). Conclusion: Comparing the corneal confocal microscopic characteristics after DMEK with those after DSAEK and their correlation with visual outcome at 6 months, we hypothesized that the presence of a third reflectivity peak in the Z-scan curves of DSAEK patients could justify the poorer visual outcome with this endothelial surgery than with DMEK.

scholarly journals Corneal Subbasal Plexus in Eyes with Fuchs’ Endothelial Corneal Dystrophy after Two Different Endothelial Surgeries

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Irene Abicca ◽  
Marta Gilardi ◽  
Daniela Giannini ◽  
Rossella Anna Maria Colabelli Gisoldi ◽  
Augusto Pocobelli ◽  
...  
Keyword(s):  
Corneal Thickness ◽  
Corneal Dystrophy ◽  
Nerve Fibers ◽  
Endothelial Keratoplasty ◽  
Chi Square ◽  
Corneal Incision ◽  
Corneal Confocal Microscopy ◽  
Corrected Distance Visual Acuity ◽  
Different Types

Purpose. To evaluate the morphological features and density of corneal subbasal plexus (SBP) using in vivo corneal confocal microscopy (IVCCM) in patients affected by Fuchs’ endothelial corneal dystrophy (FECD) six months after Descemet membrane endothelial keratoplasty (DMEK) and Descemet-stripping automated endothelial keratoplasty (DSAEK). Methods. We included patients affected by FECD, requiring corneal endothelial surgery due to corneal oedema occurred from 3 to 6 months. 7 eyes underwent DMEK and 7 eyes DSAEK. All patients performed IVCCM preoperative and in six months postoperative. We analyzed SBP parameters, using CS4 Nerves Tracking Tool, and we studied the differences between the two endothelial keratoplasties. Results. Comparing the eyes treated with DMEK with those treated with DSAEK, preoperative corneal thickness, corrected distance visual acuity (CDVA), and age were similar in both groups. SBP was not detectable at preoperative IVCCM in any eye. Postoperatively, the nerve fibers length, the nerve fibers density, the tortuosity, and the number of fibers and of branching did not differ in the eyes that underwent DMEK compared to DSAEK. The corneal beadings density was higher after DMEK than DSAEK, and this difference was statistically significant ( P  = 0.004). The type of endothelial keratoplasty was not associated with the presence or absence of postoperative corneal SBP (Pearson’ chi-square, 0.755). Conclusions. Postoperative corneal reinnervation should be easily and noninvasively studied using IVCCM. Morphological postoperative features of SBP did not differ between two different types of endothelial keratoplasty, DMEK and DSAEK, despite the different sizes of the corneal incision. The lower beading density in the DSAEK group should be the consequence of a different distribution of mitochondria along the nerve fibers, as expression of a supposed higher metabolic distress in the DSAEK group.

Outcomes of Hemi-Descemet Membrane Endothelial Keratoplasty for Fuchs Endothelial Corneal Dystrophy

Cornea ◽  
2018 ◽  
Vol 37 (7) ◽  
pp. 854-858 ◽  
Author(s):  
Rénuka S. Birbal ◽  
Shugi Hsien ◽  
Vasiliki Zygoura ◽  
Jack S. Parker ◽  
Lisanne Ham ◽  
...  
Keyword(s):  
Corneal Dystrophy ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane

In vivo corneal confocal microscopy in keratoconus

2005 ◽  
Vol 25 (3) ◽  
pp. 254-260 ◽  
Author(s):  
Joanna G. Hollingsworth ◽  
Nathan Efron ◽  
Andrew B. Tullo
Keyword(s):  
Confocal Microscopy ◽  
Corneal Confocal Microscopy

scholarly journals The use of confocal microscopy in experimental studies and clinical practice of an endocrinologist: modern opportunities

2019 ◽  
Vol 65 (3) ◽  
pp. 174-183
Author(s):  
Natalya G. Mokrysheva ◽  
Sergey L. Kiselev ◽  
Natalia V. Klementieva ◽  
Anna M. Gorbacheva ◽  
Ivan I. Dedov
Keyword(s):  
Confocal Microscopy ◽  
Experimental Studies ◽  
Three Dimensional ◽  
Imaging Method ◽  
New Methods ◽  
Corneal Confocal Microscopy ◽  
Endocrine Ophthalmopathy ◽  
Fundamental Research

Confocal microscopy is a modern imaging method that provides ample opportunities for in vitro and in vivo research. The clinical part of the review focuses on well-established techniques, such as corneal confocal microscopy for the diagnosis of diabetic neuropathy or endocrine ophthalmopathy; new methods are briefly described (intraoperative evaluation of tissues obtained by removing pituitary adenomas, thyroid and parathyroid glands). In the part devoted to fundamental research, the use of confocal microscopy to characterize the colocalization of proteins, as well as three-dimensional intracellular structures and signaling pathways in vivo, is considered. Indicators of intracellular calcium are analyzed.

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