scholarly journals Chemoenzymatic Synthesis of Two New Halogenated Coumarin Glycosides as Potential Antifungal Agents

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Liangbin Zhou ◽  
Ling Liu ◽  
Tian Tian ◽  
Bailin Xue ◽  
Rongmin Yu
Keyword(s):  
Hairy Roots ◽  
Antifungal Agents ◽  
Chemoenzymatic Synthesis ◽  
Polygonum Multiflorum ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Transgenic Hairy Roots ◽  
Exogenous Sugar

Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7- O- β-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5- O- β-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.

Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

2008 ◽  
Vol 19 (1) ◽  
pp. 76-78 ◽  
Author(s):  
Rong Min Yu ◽  
Liang Bin Zhou ◽  
Chun Yan Yan ◽  
Guo Yan Duan ◽  
Yu Zhao
Keyword(s):  
Hairy Roots ◽  
Polygonum Multiflorum ◽  
Transgenic Hairy Roots

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst

Production of biodiesel by immobilized whole-cell biocatalyst

2008 ◽  
Vol 136 ◽  
pp. S385-S386
Author(s):  
Jyh-Ping Chen ◽  
Shu-Chin Chang
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst
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