scholarly journals Evaluation of plasma Epstein–Barr virus DNA as a biomarker for Epstein–Barr virus–associated Hodgkin lymphoma

2021 ◽  
Vol 9 ◽  
pp. 205031212110549
Author(s):  
Masuma Khatun Usha ◽  
Jasmine Akhter Jahan ◽  
SM Rashed Ul Islam ◽  
Ferdousy Begum ◽  
Chowdhury Rafiqul Ahsan ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Hodgkin Lymphoma ◽  
Epstein Barr Virus ◽  
Dna Detection ◽  
Quantitative Polymerase Chain Reaction ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 1 ◽  
Tumor Biopsy ◽  
Barr Virus ◽  
Epstein Barr

Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causative agent for Hodgkin lymphoma. Although immunostaining of the tumor biopsy is the standard method for diagnosis of Epstein–Barr virus-driven Hodgkin lymphoma, the invasiveness of the procedure renders it difficult and less desirable for the patients. Therefore, we designed this study to evaluate the efficiency of plasma Epstein–Barr virus DNA detection as an alternative diagnostic and prognostic method for Epstein–Barr virus–associated Hodgkin lymphoma. Methods: This analytical cross-sectional study was conducted during March 2017 to December 2018 including 43 Hodgkin lymphoma patients diagnosed histopathologically followed by the latent membrane protein-1 immunohistochemistry to determine their Epstein–Barr virus association. Plasma Epstein–Barr virus DNA in these samples was measured using quantitative polymerase chain reaction (qPCR). Results: Of total, 29 (67.44%) patients tested positive for plasma Epstein–Barr virus DNA. On comparing results of latent membrane protein-1 immunohistochemistry (IHC) with plasma Epstein–Barr virus DNA, plasma Epstein–Barr virus DNA was found in 25 of 30 patients with latent membrane protein-1 expression and 4 of 13 patients without latent membrane protein-1 expression. The sensitivity and the specificity of plasma Epstein–Barr virus DNA detection with respect to latent membrane protein-1 IHC were found to be 83.33% and 69.23%, respectively ( p = 0.0014). Conclusion: Determination of plasma Epstein–Barr virus DNA was found to be highly sensitive and specific in characterizing Epstein–Barr virus–associated Hodgkin lymphoma, suggesting that this diagnostic method holds promise as an alternative and more convenient method of diagnosis compared with tissue biopsy.

scholarly journals Epstein-Barr virus latent membrane protein-1 expression in Hodgkin lymphoma

2018 ◽  
Vol 11 (3) ◽  
pp. 203
Author(s):  
Ferdousy Begum ◽  
Prabir Kumar Saha ◽  
Tasmina Enam ◽  
Mohammed Kamal
Keyword(s):  
Membrane Protein ◽  
Hodgkin Lymphoma ◽  
Epstein Barr Virus ◽  
Disease Process ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 1 ◽  
Classical Hodgkin Lymphoma ◽  
Mixed Cellularity ◽  
Barr Virus ◽  
Epstein Barr

<p class="Abstract">Expression of latent membrane protein-1 of Epstein-Barr virus is documented in Hodgkin lymphoma indicating its relationship in disease process. A total of 50 cases of Hodgkin lymphoma were analyzed for latent membrane protein-1 expression by immunohistochemistry. The mean age was 28.7 years with male predominance. Mixed cellularity classical Hodgkin lymphoma was the commonest subtype (50% cases). Out of 50 cases, 48% cases were found positive for latent membrane protein. Of the five histologic subtypes, all cases of lymphocyte depleted classical Hodgkin lymphoma showed positivity which was followed by 60% positivity of mixed cellularity classical Hodgkin lymphoma. Association of latent membrane protein-1 expression in relation to different age group, site of involvement and subtypes of Hodgkin lymphoma were found statistically insignificant while latent membrane protein-1 expression was significantly higher in male than female.</p>

558 Epstein Barr virus latent membrane protein 1 in Hodgkin lymphoma

2010 ◽  
Vol 8 (5) ◽  
pp. 142
Author(s):  
L.K. Lee ◽  
P.A. Chandran ◽  
M. Vockerodt ◽  
C.B. Woodman ◽  
P.G. Murray ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Hodgkin Lymphoma ◽  
Epstein Barr Virus ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 1 ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Latent Membrane Protein

scholarly journals Sequence Variations of Epstein-Barr Virus-Encoded Small Noncoding RNA and Latent Membrane Protein 1 in Hematologic Tumors in Northern China

Intervirology ◽  
2021 ◽  
Vol 64 (2) ◽  
pp. 69-80
Author(s):  
Hai-Yu Wang ◽  
Lingling Sun ◽  
Ping Li ◽  
Wen Liu ◽  
Zhong-Guang Zhang ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Nested Pcr ◽  
Noncoding Rna ◽  
Epstein Barr Virus ◽  
Northern China ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 1 ◽  
Small Noncoding Rna ◽  
Barr Virus ◽  
Epstein Barr

<b><i>Objective:</i></b> To investigate the relationship between hematologic tumors and Epstein-Barr virus (EBV)-encoded small noncoding RNA (EBER) variations as well as latent membrane protein 1 (LMP1) variations. <b><i>Methods:</i></b> Patients with leukemia and myelodysplastic syndrome (MDS) were selected as subjects. Genotypes 1/2 and genotypes F/f were analyzed using the nested PCR technology, while EBER and LMP1 subtypes were analyzed by the nested PCR and DNA sequencing. <b><i>Results:</i></b> Type 1 was more dominant than type 2, found in 59 out of 82 (72%) leukemia and in 31 out of 35 (88.6%) MDS, while type F was more prevalent than type f in leukemia (83/85, 97.6%) and MDS (29/31, 93.5%) samples. The distribution of EBV genotypes 1/2 was not significantly different among leukemia, MDS, and healthy donor groups, neither was that of EBV genotypes F/f. EB-6m prototype was the dominant subtype of EBER in leukemia and MDS (73.2% [30/41] and 83.3% [10/12], respectively). The frequency of EB-6m was lower than that of healthy people (96.7%, 89/92), and the difference was significant (<i>p</i> &#x3c; 0.05). China 1 subtype was the dominant subtype of LMP1 in leukemia and MDS (70% [28/40] and 90% [9/10], respectively), and there was no significant difference in the distribution of LMP1 subtypes among the 3 groups (<i>p</i> &#x3e; 0.05). <b><i>Conclusion:</i></b> The distribution of EBV 1/2, F/f, EBER, and LMP1 subtypes in leukemia and MDS was similar to that in the background population in Northern China, which means that these subtypes may be rather region-restricted but not associated with leukemia and MDS pathogenesis.

Epstein-Barr virus latent membrane protein 1 mRNA is expressed in a significant proportion of patients with chronic lymphocytic leukemia

Cancer ◽  
2010 ◽  
Vol 116 (4) ◽  
pp. 880-887 ◽  
Author(s):  
Jeffrey J. Tarrand ◽  
Michael J. Keating ◽  
Apostolia M. Tsimberidou ◽  
Susan O'Brien ◽  
Rocco P. LaSala ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Membrane Protein ◽  
Epstein Barr Virus ◽  
Significant Proportion ◽  
Latent Membrane Protein ◽  
Lymphocytic Leukemia ◽  
Latent Membrane Protein 1 ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Latent Membrane Protein
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