scholarly journals Immunohistochemical localization of thyroid hormone in rat thyroid gland.

1978 ◽  
Vol 26 (12) ◽  
pp. 1121-1124 ◽  
Author(s):  
M Wilson ◽  
K R Hitchcock ◽  
R A DeLellis
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Thyroid Hormones ◽  
Parathyroid Gland ◽  
Immunohistochemical Localization ◽  
Follicular Cells ◽  
Adult Rat ◽  
Apical Cytoplasm ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

Direct and indirect immunofluorescence techniques were used to localize the thyroid hormones triidothyronine (T3) and thyroxine (T4) in adult rat thyroid gland. Optimum dilutions of the antisera were established and four tissue fixatives were investigated for usefulness in this technique. Use of antibodies specific for either T3 or T4 resulted in brilliant fluorescence in the colloid pools and apical cytoplasm of follicular cells. In all cases, the adjacent parathyroid gland was devoid of fluorescence. This report demonstrates that these dipeptide hormones can be localized by using immunofluorescence techniques.

scholarly journals Immunocytochemical localization of cathepsins B, H, L, and T4 in follicular cells of rat thyroid gland.

1989 ◽  
Vol 37 (5) ◽  
pp. 691-696 ◽  
Author(s):  
Y Uchiyama ◽  
T Watanabe ◽  
M Watanabe ◽  
Y Ishii ◽  
H Matsuba ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Thyroid Tissue ◽  
Double Immunostaining ◽  
Follicular Cells ◽  
Thin Sections ◽  
Dense Granules ◽  
Dense Bodies ◽  
Rat Thyroid ◽  
Monospecific Antibodies ◽  
Rat Thyroid Gland

To localize cathepsins B, H, and L in follicular cells of rat thyroid gland, we applied immunocytochemistry to the thyroid tissue using their respective monospecific antibodies. On serial semi-thin sections, cathepsins B, H, and L were localized in granules of various sizes located throughout the cytoplasm, whereas T4 was detected in larger granules located in the apical and supranuclear regions. By electron microscopy, cathepsins B, H, and L were localized in large less-dense granules (so-called colloid droplets) and in dense bodies of various sizes, whereas T4 was localized more intensely in large less-dense granules than in smaller dense bodies. By double immunostaining using an immunogold method, cathepsins H and B or L were co-localized in the same cytoplasmic granules. Moreover, immunoblotting demonstrated that proteins similar to cathepsins B, H, and L in the liver are present in the thyroid gland. These results suggest that cathepsins B, H, and L participate not only in degradation of thyroglobulin but in maturation of thyroid hormones, although it remains unknown whether all of them participate in the maturation process.

Autoradiographic Localization of Slow-Turnover Iodocompounds Within the Follicular Cells of the Rat Thyroid Gland

Endocrinology ◽  
1975 ◽  
Vol 97 (4) ◽  
pp. 978-984 ◽  
Author(s):  
A. HAEBERLI ◽  
H. STUDER ◽  
H. KOHLER ◽  
H. BüRGI ◽  
H. ENGLER
Keyword(s):  
Thyroid Gland ◽  
Follicular Cells ◽  
Rat Thyroid ◽  
Rat Thyroid Gland ◽  
Slow Turnover

Electron Microscope Immunohistochemical Localization of Thyroglobulin in the Rat Thyroid Gland

Endocrinology ◽  
1976 ◽  
Vol 98 (5) ◽  
pp. 1253-1259 ◽  
Author(s):  
GEORGES PELLETIER ◽  
ROMANO PUVIANI ◽  
JEAN H. DUSSAULT
Keyword(s):  
Electron Microscope ◽  
Thyroid Gland ◽  
Immunohistochemical Localization ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

Measurement of thyroid hormone in the rat thyroid gland by radioimmunoassay

1991 ◽  
Vol 192 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Philippe Chomard ◽  
Nicole Autissier
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

COMPARISON OF THE EFFECTS OF VARIOUS GOITROGENS ON THE BIOSYNTHESIS OF THYROID HORMONES IN VITRO

1961 ◽  
Vol 36 (2) ◽  
pp. 212-220 ◽  
Author(s):  
Shiro Iino
Keyword(s):  
Thyroid Gland ◽  
Thyroid Hormones ◽  
Potent Inhibitor ◽  
Relative Activity ◽  
Antithyroid Drugs ◽  
Modes Of Action ◽  
Antithyroid Activity ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

ABSTRACT The effect of various goitrogens on in vitro biosynthesis of thyroid horhomes was studied. The compounds in decreasing order of relative activity to completely inhibit DIT formation were 1-methyl-2-mercaptoimidazole (MIA), thiouracil (TU); propylthiouracil PTU) and goitrin; thiourea and methylthiouracil (MTU). Those in decreasing order of relative activity to completely inhibit MIT formation were MIA; PTU, MTU, TU and thiourea; goitrin. Of the compounds tested MIA was thus the most potent inhibitor of the rat thyroid gland in vitro. The conversion of monoiodotyrosine to diiodotyrosine was more sensitive to every goitrogen than the iodination of tyrosine to form monoiodotyrosine. The modes of action of these antithyroid drugs seemed to be approximately the same, except for goitrin, which showed very weak inhibition of MIT formation. Progoitrin, the precursor of goitrin, did not seem to possess any antithyroid activity.

Immunohistochemical localization of thyroxine (T4) and triiodothyronine (T3) in the rat thyroid gland under various experimental conditions

1983 ◽  
Vol 103 (2) ◽  
pp. 235-240 ◽  
Author(s):  
Akira Kawaoi ◽  
Tadao Okano ◽  
Norimichi Nemoto ◽  
Toshio Shikata
Keyword(s):  
Thyroid Gland ◽  
Functional State ◽  
Immunohistochemical Localization ◽  
Follicular Epithelium ◽  
Paraffin Sections ◽  
Experimental Conditions ◽  
Functional States ◽  
Rat Thyroid ◽  
Rat Thyroid Gland ◽  
Immunoenzyme Technique

Abstract. Detection of T4 and T3 in paraffin sections of the rat thyroid gland in various functional states was attempted by an immunoenzyme technique. Both hormones showed a similar localization, being detected mainly in the colloid in the follicular lumen, and sometimes in certain regions of the cytoplasm of the follicular epithelium. Following administration of TSH, their stainability was increased, and the localization within the cytoplasm became more remarkable. Decrease or disappearance of stainability was observed after hypophysectomy or administration of PTU. These findings support the view that the immunostainability of T4 and T3 is closely correlated with the functional state of the thyroid gland.

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