Autoradiographic Localization of Slow-Turnover Iodocompounds Within the Follicular Cells of the Rat Thyroid Gland

Endocrinology ◽  
1975 ◽  
Vol 97 (4) ◽  
pp. 978-984 ◽  
Author(s):  
A. HAEBERLI ◽  
H. STUDER ◽  
H. KOHLER ◽  
H. BüRGI ◽  
H. ENGLER
Keyword(s):  
Thyroid Gland ◽  
Follicular Cells ◽  
Rat Thyroid ◽  
Rat Thyroid Gland ◽  
Slow Turnover

scholarly journals Immunocytochemical localization of cathepsins B, H, L, and T4 in follicular cells of rat thyroid gland.

1989 ◽  
Vol 37 (5) ◽  
pp. 691-696 ◽  
Author(s):  
Y Uchiyama ◽  
T Watanabe ◽  
M Watanabe ◽  
Y Ishii ◽  
H Matsuba ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Thyroid Tissue ◽  
Double Immunostaining ◽  
Follicular Cells ◽  
Thin Sections ◽  
Dense Granules ◽  
Dense Bodies ◽  
Rat Thyroid ◽  
Monospecific Antibodies ◽  
Rat Thyroid Gland

To localize cathepsins B, H, and L in follicular cells of rat thyroid gland, we applied immunocytochemistry to the thyroid tissue using their respective monospecific antibodies. On serial semi-thin sections, cathepsins B, H, and L were localized in granules of various sizes located throughout the cytoplasm, whereas T4 was detected in larger granules located in the apical and supranuclear regions. By electron microscopy, cathepsins B, H, and L were localized in large less-dense granules (so-called colloid droplets) and in dense bodies of various sizes, whereas T4 was localized more intensely in large less-dense granules than in smaller dense bodies. By double immunostaining using an immunogold method, cathepsins H and B or L were co-localized in the same cytoplasmic granules. Moreover, immunoblotting demonstrated that proteins similar to cathepsins B, H, and L in the liver are present in the thyroid gland. These results suggest that cathepsins B, H, and L participate not only in degradation of thyroglobulin but in maturation of thyroid hormones, although it remains unknown whether all of them participate in the maturation process.

scholarly journals Immunohistochemical localization of thyroid hormone in rat thyroid gland.

1978 ◽  
Vol 26 (12) ◽  
pp. 1121-1124 ◽  
Author(s):  
M Wilson ◽  
K R Hitchcock ◽  
R A DeLellis
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Thyroid Hormones ◽  
Parathyroid Gland ◽  
Immunohistochemical Localization ◽  
Follicular Cells ◽  
Adult Rat ◽  
Apical Cytoplasm ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

Direct and indirect immunofluorescence techniques were used to localize the thyroid hormones triidothyronine (T3) and thyroxine (T4) in adult rat thyroid gland. Optimum dilutions of the antisera were established and four tissue fixatives were investigated for usefulness in this technique. Use of antibodies specific for either T3 or T4 resulted in brilliant fluorescence in the colloid pools and apical cytoplasm of follicular cells. In all cases, the adjacent parathyroid gland was devoid of fluorescence. This report demonstrates that these dipeptide hormones can be localized by using immunofluorescence techniques.

Effects of toxic doses of a novel histamine (H2) antagonist on the rat thyroid gland

1987 ◽  
Vol 25 (10) ◽  
pp. 787-794 ◽  
Author(s):  
C.G. Brown ◽  
R.F. Harland ◽  
I.R. Major ◽  
C.K. Atterwill
Keyword(s):  
Thyroid Gland ◽  
H2 Antagonist ◽  
Histamine H2 Antagonist ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

CORRELATION OF IODIDE TRANSPORT WITH Na+, K+ -ATPase, HCO−3-ATPase AND CARBONIC ANHYDRASE ACTIVITIES IN DIFFERENT FUNCTIONAL STATES OF THE RAT THYROID GLAND

1982 ◽  
Vol 92 (3) ◽  
pp. 371-379 ◽  
Author(s):  
S.-Y. CHOW ◽  
J. W. KEMP ◽  
D. M. WOODBURY
Keyword(s):  
Thyroid Gland ◽  
Carbonic Anhydrase ◽  
Protein Content ◽  
Chronic Treatment ◽  
Cell Water ◽  
Iodide Uptake ◽  
G Cell ◽  
Functional States ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

The effects of thyrotrophin, hypophysectomy, and chronic treatment with thyroxine and methimazole on radioiodide uptake (thyroid/plasma (T/P) 125I− ratio), protein and DNA contents and activities of Na+,K+ -ATPase, HCO−3-ATPase, and carbonic anhydrase (CA) of rat thyroid gland were evaluated. Thyrotrophin given to intact rats slightly increased thyroid iodide uptake, did not affect protein or DNA content, and slightly inhibited CA activity (units/g cell water). Hypophysectomy markedly decreased T/P 125I− ratio, increased protein content, decreased activity of Na+,K+-ATPase, and slightly increased HCO−3-ATPase (nmol/mg DNA per min) and CA (units/g cell water) activities. Thyro-trophin given to hypophysectomized rats (as compared with untreated hypophysectomized control animals) markedly increased T/P 125I− ratio, slightly decreased protein content and decreased Na+,K+-ATPase and CA activities. Chronic treatment with methimazole increased T/P 125I− ratio, decreased protein content, markedly increased Na+,K+-ATPase and HCO−3-ATPase activities, and decreased CA activity. Chronic treatment with thyroxine, in contrast, decreased T/P 125I− ratio, decreased Na+,K+-ATPase activity, and increased CA activity. There was a significant inverse correlation between T/P 125I− ratio and CA activity in follicular cells for the various induced functional states of the thyroid.

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