Expression and induction by dexamethasone of ABC transporters and nuclear receptors in a human T-lymphocyte cell line

2012 ◽  
Vol 24 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Sandra Manceau ◽  
Carole Giraud ◽  
Xavier Declèves ◽  
Frédéric Batteux ◽  
Christiane Chéreau ◽  
...  
Keyword(s):  
Cell Line ◽  
Nuclear Receptors ◽  
T Lymphocyte ◽  
Abc Transporters ◽  
Human T Lymphocyte ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

scholarly journals Human T lymphocyte cell line producing colony-stimulating activity

Blood ◽  
1978 ◽  
Vol 52 (5) ◽  
pp. 1068-1072 ◽  
Author(s):  
DW Golde ◽  
SG Quan ◽  
MJ Cline
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
T Lymphocyte ◽  
Maximal Activity ◽  
Blood Leukocytes ◽  
Murine Bone Marrow ◽  
Colony Stimulating Activity ◽  
Human T Lymphocyte ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

Abstract We derived a permanent human T lymphocyte cell line that elaborates a potent colony-stimulating activity (CSA). The line was established with spleen cells from a patient with a T lymphocyte variant of hairy-cell leukemia. These cells form rosettes with sheep erythrocytes, show a proliferative response to phytohemagglutinin, and are lysed by antithymocyte globulin. They do not synthesize immunoglobulin, nor do they contain Epstein-Barr virus. CSA is regularly detected in the supernatant medium after 3 days culture. In the presence of PHA there is augmented elaboration of CSA; maximal activity is reached by 2 days and is 20% greater than that produced by a feeder layer of 1 X 10(6) peripheral blood leukocytes. One microliter of the supernatant material stimulated colony formation from the light-density nonadherent fraction of human bone marrow; there was maximal activity between 10 and 50 microliter/ml. Conditioned medium from these cells has little effect in stimulating CFU-C from murine bone marrow. The availability of a human T lymphocyte line producing CSA will provide a source for large quantities of the lymphocyte-derived hormone and permit a definition of factors modulating the interaction of T lymphocytes with granulocyte and monocyte stem cells.

Immunomodulating effects of water-soluble extracts of traditional French Alps cheeses on a human T-lymphocyte cell line

2006 ◽  
Vol 16 (12) ◽  
pp. 1505-1514 ◽  
Author(s):  
Christèle Durrieu ◽  
Pascal Degraeve ◽  
Stéphane Chappaz ◽  
Adèle Martial-Gros
Keyword(s):  
Cell Line ◽  
T Lymphocyte ◽  
Water Soluble ◽  
French Alps ◽  
Human T Lymphocyte ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

Production of inositol pentakisphosphate in a human T lymphocyte cell line

1987 ◽  
Vol 145 (2) ◽  
pp. 895-902 ◽  
Author(s):  
Stanford J. Stewart ◽  
Linda L. Kelley ◽  
Frances S. Powers
Keyword(s):  
Cell Line ◽  
T Lymphocyte ◽  
Human T Lymphocyte ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

scholarly journals Human T lymphocyte cell line producing colony-stimulating activity

Blood ◽  
1978 ◽  
Vol 52 (5) ◽  
pp. 1068-1072 ◽  
Author(s):  
DW Golde ◽  
SG Quan ◽  
MJ Cline
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
T Lymphocyte ◽  
Maximal Activity ◽  
Blood Leukocytes ◽  
Murine Bone Marrow ◽  
Colony Stimulating Activity ◽  
Human T Lymphocyte ◽  
Lymphocyte Cell ◽  
Lymphocyte Cell Line

We derived a permanent human T lymphocyte cell line that elaborates a potent colony-stimulating activity (CSA). The line was established with spleen cells from a patient with a T lymphocyte variant of hairy-cell leukemia. These cells form rosettes with sheep erythrocytes, show a proliferative response to phytohemagglutinin, and are lysed by antithymocyte globulin. They do not synthesize immunoglobulin, nor do they contain Epstein-Barr virus. CSA is regularly detected in the supernatant medium after 3 days culture. In the presence of PHA there is augmented elaboration of CSA; maximal activity is reached by 2 days and is 20% greater than that produced by a feeder layer of 1 X 10(6) peripheral blood leukocytes. One microliter of the supernatant material stimulated colony formation from the light-density nonadherent fraction of human bone marrow; there was maximal activity between 10 and 50 microliter/ml. Conditioned medium from these cells has little effect in stimulating CFU-C from murine bone marrow. The availability of a human T lymphocyte line producing CSA will provide a source for large quantities of the lymphocyte-derived hormone and permit a definition of factors modulating the interaction of T lymphocytes with granulocyte and monocyte stem cells.

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