scholarly journals Fluorescence of Megakaryocytes in Idiopathic Thrombocytopenic Purpura (ITP) Stained with Fluorescent Antiglobulin Serum

Blood ◽  
1962 ◽  
Vol 19 (6) ◽  
pp. 664-675 ◽  
Author(s):  
JAMES L. MCKENNA ◽  
ANTHONY V. PISCIOTTA ◽  
Jean Hinz
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Thrombocytopenic Purpura ◽  
Chronic Itp ◽  
Neonatal Thrombocytopenia ◽  
Acute Itp

Abstract 1. A technic is described for the direct and indirect visualization of affinity between fluorescein labeled antiglobulin serum and megakaryocytes. 2. Fluorescent megakaryocytes have been found with this technic in the marrow of some patients with chronic ITP, but not in acute ITP. It was possiple to confer fluorescence on normal megakaryocytes by incubating them with sera from patients with chronic ITP, and from a woman who had a child with neonatal thrombocytopenia. 3. Similar reactions were noted in three patients with systemic lupus erythematosus. 4. These findings indicate adherence of a humoral substance (antibody?) for megakaryocytes, which may have etiologic significance in chronic ITP.

scholarly journals In Vitro Detection of Platelet Antibody in Patients with Idiopathic Thrombocytopenic Purpura and Systemic Lupus Erythematosus

Blood ◽  
1969 ◽  
Vol 33 (6) ◽  
pp. 795-812 ◽  
Author(s):  
SIMON KARPATKIN ◽  
GREGORY W. SISKIND
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Lupus Erythematosus ◽  
Detection Sensitivity ◽  
Coagulation Cascade ◽  
Systemic Lupus ◽  
Platelet Factor ◽  
Thrombocytopenic Purpura ◽  
Platelet Antibody

Abstract Two technics have been developed, which make possible the in vitro detection of platelet antibody in patients with idiopathic thrombocytopenic purpura (ITP), and systemic lupus erythematosus (SLE). The dextran-platelet agglutination technic takes advantage of the enhancement of platelet agglutination in the presence of dextran which brings about a threefold increase in simple agglutination. The Platelet-Factor-3 test measures the ability of platelet antibody to injure platelets and in so doing, make Platelet Factor 3 available to the coagulation cascade, thus accelerating the fibrin clotting time. This technic, when compared to the simple platelet agglutination test, increases the detection sensitivity of antiplatelet factor(s) twenty-fivefold. The antiplatelet factor(s) as measured by these technics was found to be an immunoglobulin of the IgG class. The antiplatelet factor(s) was present in the sera of 85 per cent of patients with ITP and 88 per cent of patients with SLE. This antiplatelet factor(s) was absent in 25 consecutive healthy controls and in 22 out of 26 patients with thrombocytopenia not due to ITP or SLE. Although 88 per cent of patients with SLE had demonstrable antiplatelet activity in their serum only 2 out of 33 were thrombocytopenic. These observations suggested that a compensated thrombocytolytic state was present in these patients. This conclusion was consistent with the good correlation between an elevated large platelet index and the presence of antiplatelet factor in these patients.

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