scholarly journals Distinct Recirculating and Non-Recirculating B-Lymphocyte Pools in the Peripheral Blood Are Defined by Coordinated Expression of CD21 and L-Selectin

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4865-4875 ◽  
Author(s):  
Alan J. Young ◽  
Wendy L. Marston ◽  
Mark Dessing ◽  
Lisbeth Dudler ◽  
Wayne R. Hein
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Surface Expression ◽  
Peripheral Blood Lymphocytes ◽  
Phenotypic Analysis ◽  
Phenotypic Properties ◽  
Blood Lymphocytes ◽  
The Status ◽  
Coordinated Expression

Abstract The continual recirculation of lymphocytes between the blood, tissues, and lymph is essential for the coordination and dissemination of immune responses. We have compared the functional and phenotypic properties of lymphocytes isolated from blood and lymph, the two major migratory populations. Lymph-borne lymphocytes migrated readily into the lymphatic recirculation pathway, but greater than one third of all peripheral blood lymphocytes (PBLs) were excluded from the lymphatic circuit and showed an enhanced migration to the spleen. Phenotypic analysis showed that most non-recirculating PBLs were B cells. The migration competence of B cells correlated with the surface expression of CD21 and L-selectin; recirculating B cells expressed both of these molecules, whereas non-recirculating B cells lacked both antigens. These results establish that blood contains distinct pools of lymphocytes that differ in their recirculation competence. Clearly, blood sampling is not an efficient method to directly measure the status of the recirculating immune system, and implies important constraints and restrictions in the interpretation of experimental or clinical data that include phenotypic and quantitative analyses of blood lymphocytes.

scholarly journals Distinct Recirculating and Non-Recirculating B-Lymphocyte Pools in the Peripheral Blood Are Defined by Coordinated Expression of CD21 and L-Selectin

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4865-4875
Author(s):  
Alan J. Young ◽  
Wendy L. Marston ◽  
Mark Dessing ◽  
Lisbeth Dudler ◽  
Wayne R. Hein
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Surface Expression ◽  
Peripheral Blood Lymphocytes ◽  
Phenotypic Analysis ◽  
Phenotypic Properties ◽  
Blood Lymphocytes ◽  
The Status ◽  
Coordinated Expression

The continual recirculation of lymphocytes between the blood, tissues, and lymph is essential for the coordination and dissemination of immune responses. We have compared the functional and phenotypic properties of lymphocytes isolated from blood and lymph, the two major migratory populations. Lymph-borne lymphocytes migrated readily into the lymphatic recirculation pathway, but greater than one third of all peripheral blood lymphocytes (PBLs) were excluded from the lymphatic circuit and showed an enhanced migration to the spleen. Phenotypic analysis showed that most non-recirculating PBLs were B cells. The migration competence of B cells correlated with the surface expression of CD21 and L-selectin; recirculating B cells expressed both of these molecules, whereas non-recirculating B cells lacked both antigens. These results establish that blood contains distinct pools of lymphocytes that differ in their recirculation competence. Clearly, blood sampling is not an efficient method to directly measure the status of the recirculating immune system, and implies important constraints and restrictions in the interpretation of experimental or clinical data that include phenotypic and quantitative analyses of blood lymphocytes.

Phenotypic analysis of skin infiltrates in comparison with peripheral blood lymphocytes, spleen cells and thymocytes in early avian scleroderma

1991 ◽  
Vol 4 (4) ◽  
pp. 577-593 ◽  
Author(s):  
Matthias S. Gruschwitz ◽  
Susanne Moormann ◽  
Guido Krömer ◽  
Roswitha Sgonc ◽  
Herrmann Dietrich ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Spleen Cells ◽  
Phenotypic Analysis ◽  
Blood Lymphocytes

Anthracyclines trigger apoptosis of both G0 G1 and cycling peripheral blood lymphocytes and induce massive deletion of mature T and B cells

1999 ◽  
Vol 91 (7) ◽  
pp. 557-557
Author(s):  
Carole Ferraro ◽  
Laurence Quemeneur ◽  
Annie-France Prigent ◽  
Catherine Taverne ◽  
Jean-Pierre Revillard ◽  
...  
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
T And B Cells ◽  
Blood Lymphocytes

Stimulation of human peripheral blood lymphocytes by autologous EBV-infected B cells

1978 ◽  
Vol 41 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jean Viallat ◽  
Erik Svedmyr ◽  
Eitan Yefenof ◽  
George Klein ◽  
Ola Weiland
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Stimulation Of

The Effect of a 20-Minute Exposure of Colloidal Silver on the Membrane of Human Peripheral Blood Lymphocytes and Raji Cells

2000 ◽  
Vol 6 (S2) ◽  
pp. 890-891
Author(s):  
K. R. Garvin ◽  
C. J. Hintze ◽  
R. D. Bowden ◽  
D. A. Johnson ◽  
J. E. Tidwell ◽  
...  
Keyword(s):  
Cell Death ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Human Peripheral Blood ◽  
Scientific Evidence ◽  
Peripheral Blood Lymphocytes ◽  
Colloidal Silver ◽  
Normal Peripheral Blood ◽  
Raji Cells ◽  
Blood Lymphocytes

Apoptosis (programmed cell death) is an important form of physiological cell death displayed under divergent conditions by an enormous variety of tissues. Apoptosis can be characterized by cell shrinkage, membrane blebbing, and DNA fragmentation (1). Data from recent reports have suggested that many types of cancer are accompanied by the loss of apoptotic ability (2). Many manufacturers have hailed colloidal silver as an effective treatment of many diseases including cancer (3). The FDA has recently finalized a rule stating that drug products containing colloidal silver or silver salts are not recognized as safe and effective. They conclude that product labels claiming that scientific evidence suggests otherwise are incorrect (4). In this study we undertook to examine the effects of colloidal silver on both Raji cells, a B lymphocyte lymphoma, (ATCC # CCL-86) and normal, peripheral blood lymphocytes (PBL). We noted a decrease in viability of 90% in both Raji cells and lymphocytes after exposure to colloidal silver.

scholarly journals Determination of glutathione in lymphocytes and possible association of redox state and proliferative capacity of lymphocytes

1981 ◽  
Vol 198 (3) ◽  
pp. 571-579 ◽  
Author(s):  
R J Noelle ◽  
D A Lawrence
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Chemical Oxidation ◽  
Peripheral Blood Lymphocytes ◽  
Human Erythrocytes ◽  
T And B Cells ◽  
Inhibition Of Proliferation ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

The glutathione (GSH) content of mouse T- and B-cells was determined and compared with the GSH content of human peripheral blood lymphocytes and human erythrocytes. Owing to the difficulty of obtaining large numbers of purified lymphocytes, a technique was developed to measure picomolar quantities of GSH. By this technique, mouse T- and B-cells, as well as mouse peripheral-blood lymphocytes, were found to contain approx. 30% of the GSH found in human peripheral-blood lymphocytes. The concanavalin A response of human peripheral-blood lymphocytes and human spleen cells was insensitive to 2-mercaptoethanol as well as to culture in 17% O2, whereas mouse lymphocyte responses were altered by 2-mercaptoethanol and inhibited by 17% O2. The capacity of human peripheral-blood lymphocytes, human erythrocytes, mouse T-cells and mouse B-cells to regenerate GSH stores after chemical oxidation by diamide was tested, and it was found that mouse cells were less capable of regenerating GSH than human erythrocytes or human peripheral-blood lymphocytes. In addition, the latter lymphocytes were less sensitive to oxidation of GSH and to inhibition of proliferation by diamide.

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