Industrialization plays a major role for the economic development of any nation. In spite of various positive aspects of industrialization, the foremost negative aspect is pollution by discharge of more waste water in to the environment. So, the aim of the present work is to analyse the phytotoxic effect of textile industry waste water on seed germination and treating the waste water using Bacterial Species and Zinc Oxide Nanoparticle. Physico-chemical parameters of dying industry waste water such as color, temperature, pH, conductivity, turbidity, total dissolved solid, total hardness, COD, BOD, oil and greases, chloride, sodium, potassium, chromium, copper, total alkalinity and zinc were analysed as per standard methods. To confirm the harmfulness, an investigation was made to study the degree of toxicity of dyeing industry effluent on seed germination and growth of Sorghum bicolor (white sorghum) Vigna unguiculata (cow pea) were selected for this study and it was placed in soil containing pots and watering with untreated industry waste water and treated waste water. The waste water was treated by two methods, one by bacterial degradation, using Pseudomonas fluroscence and the secondly, by chemical oxidation, using zinc oxide nanoparticles. After treatment their efficiency was tested with above two plants. All the above said parameter were found to be high in untreated waste water. There was a gradual decrease in the percentage of seed germination and seedling growth due to higher concentration of effluent, when compared with control. In this comparative study, methyl orange degradation by the chemical oxidation using zinc oxide nanoparticle was more effective and faster than the biological oxidation of bacterial species.