Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae)

Abstract For many years, an area-wide fruit fly control campaign against the Mexican fruit fly, Anastrepha ludens (Loew) has been implemented in some regions of Mexico and Texas, using the sterile insect technique (SIT) as its principal component. To improve the efficiency of the SIT, a genetic sexing strain based on black pupae mutation (bp) was developed for A. ludens, namely, ‘Tapachula-7’ (Tap-7 genetic sexing strains [GSSs]). This strain was introduced into the AW-IPM program recently and allows male-only releases for SIT applications. Here, we report the genetic and biological characterization of a new mutation, slow larvae (sl), which was introduced to the original translocation of the Tap-7 GSS resulting in two new GSS (slow-7 and Tap/slow-7). In both GSSs, the translocated wild-type males emerge from brown pupae that develop faster than females. The females are homozygous for sl mutation in the slow-7 GSS and homozygous for sl and bp mutations in the Tap/slow-7 GSS, reaching larval maturity 2 d after most of the wild-type males, allowing the separation of most males during pupariation. The potential use of the slow-7 and Tap/slow-7 GSSs in mass rearing and large-scale population suppression programs is discussed.

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Genetic analysis of medfly populations in an area of sterile insect technique applications

Journal of Pest Science ◽

10.1007/s10340-021-01337-8 ◽

2021 ◽

Author(s):

Rubén Sancho ◽

Ana Guillem-Amat ◽

Elena López-Errasquín ◽

Lucas Sánchez ◽

Félix Ortego ◽

...

Keyword(s):

Genetic Markers ◽

Sterile Insect Technique ◽

Ceratitis Capitata ◽

Natural Populations ◽

Low Frequency ◽

Fruit Fly ◽

Wild Populations ◽

Genetic Sexing ◽

Environmental Implications ◽

Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

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Analysis of the volatile components of a bacterial fermentation attractive to the mexican fruit fly (Anastrepha ludens).

Journal of Agricultural and Food Chemistry ◽

10.1021/jf00053a041 ◽

1995 ◽

Vol 43 (5) ◽

pp. 1348-1351 ◽

Cited By ~ 25

Author(s):

Chang-Joo Lee ◽

Albert B. DeMilo ◽

Daniel S. Moreno ◽

Adelaido J. Martinez

Keyword(s):

Fruit Fly ◽

Anastrepha Ludens ◽

Volatile Components ◽

Mexican Fruit Fly ◽

Bacterial Fermentation

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Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae)

EDIS ◽

10.32473/edis-in353-2001 ◽

1969 ◽

Vol 2004 (5) ◽

Author(s):

Howard V. Weems, Jr. ◽

John B. Heppner ◽

Thomas R. Fasulo ◽

James L. Nation

Keyword(s):

Fruit Fly ◽

Cooperative Extension Service ◽

Anastrepha Ludens ◽

Anastrepha Suspensa ◽

Mexican Fruit Fly ◽

The West ◽

University Of Florida ◽

Caribbean Fruit Fly ◽

Guava Fruit ◽

The Caribbean

The Caribbean fruit fly, Anastrepha suspensa (Loew), has also been called the Greater Antilliean fruit fly, the guava fruit fly and the Caribfly. It is a near relative of the Mexican fruit fly, Anastrepha ludens (Loew), and is one of several species of fruit flies which are indigenous to the West Indies and the larvae of which attack several kinds of tropical and subtropical fruits. This document is EENY-196 (originally published as DPI Entomology Circulars 38 and 260), one of a series of Featured Creatures from the Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published: March 2001. EENY196/IN353: Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae) (ufl.edu)

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