scholarly journals High spontaneous CD40 expression by salivary gland epithelial cells in Sjogren's syndrome: possible evidence for intrinsic activation of epithelial cells

10.1186/ar187 ◽  
2001 ◽  
Vol 3 (S2) ◽  
Author(s):  
ID Dimitriou ◽  
G Xanthou ◽  
EK Kapsogeorgou ◽  
RF Abu-Helu ◽  
HM Moutsopoulos ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Cd40 Expression ◽  
Salivary Gland Epithelial Cells ◽  
Sjögren‘S Syndrome

AB0053 Ikba regulatory protein is downregulated in sjögren’s syndrome salivary gland epithelial cells.

2013 ◽  
Vol 72 (Suppl 3) ◽  
pp. A801.3-A801
Author(s):  
M. D’amore ◽  
S. Lisi ◽  
R. De Lucro ◽  
D. Ribatti ◽  
M. Sisto
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Regulatory Protein ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Salivary Gland Epithelial Cells ◽  
Sjögren‘S Syndrome

scholarly journals Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e45689 ◽  
Author(s):  
Takafumi Kawanami ◽  
Toshioki Sawaki ◽  
Tomoyuki Sakai ◽  
Miyuki Miki ◽  
Haruka Iwao ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Salivary Gland Epithelial Cells ◽  
Sjögren‘S Syndrome

scholarly journals THU0220 AUTOPHAGY IN SJOGREN’S SYNDROME SALIVARY GLAND EPITHELIAL CELLS (SGECS) IS ASSOCIATED WITH THE SEVERITY OF INFLAMMATION AND EPITHELIAL CELLS ACTIVATION.

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 336.2-337
Author(s):  
S. Colafrancesco ◽  
C. Barbati ◽  
V. Iannizzotto ◽  
L. Mastromanno ◽  
S. Nayar ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Adhesion Molecules ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Research Support ◽  
Salivary Gland Epithelial Cells ◽  
Sjögren‘S Syndrome

Background:Sjögren’s Syndrome (SS) is characterized by chronic inflammation supported by intrinsic activation of salivary gland epithelial cells (SGECs). Eventually, apoptosis of SGECs ensues, which leads to salivary gland dysfunction and exposition of autoantigens. Autophagy is a stress coping mechanisms of cells implicated in both survival and exposition of autoantigens, and is thereby plausibly implicated in the pathogenesis of SS. At present, the exact relationship between apoptosis and autophagy in SS SGECs is unclear, as is the link between these mechanisms and SGECs activation.Objectives:To explore autophagy in SGECs from patients with SS and to evaluate its relationship with apoptosis and SGECs activation.Methods:Consecutive patients with suspected SS referring to our “Sjogren Clinic” were enrolled, and minor salivary gland (MSG) biopsies were collected for: (1) SGECs culture, (2) PCR analysis, (3) IFI analysis. In SGECs cultures, the expression of autophagy (LC3II), apoptosis (annexin V/PI) and adhesion molecules (ICAM) was investigated by flow cytometry (results expressed as mean % ± SD). The expression of the autophagy gene MAP1LC3II was evaluated by PCR (expressed as 2^deltaCT normalized to GADPH) on both MSG sections and MSG acinar and ductal epithelium samples obtained by laser capture microdissection. Tissue expression of LC3II was evaluated by IFI on SS MSG.Results:Primary SGECs cultures were established from 14 MSG obtained for diagnostic purposes (SS n=8, Sicca n=6). These cells exhibited an inverse correlation between apoptosis and autophagy (p=0.007, r=-0.784), with lower levels of apoptosis (19.7±6.5 vs 24.5±8.5, p=ns) and higher levels of autophagy (59.7±13.1 vs 54.19±19.4, p=ns) in SS compared to Sicca. In SS, MAP1LC3 was positively correlated with Focus Score (p=0.021 r=0.478); however, PCR studies did not reveal significant differences in MAP1LC3 expression between SS (n=26) and Sicca (n=15) (0.024±0.010 vs 0.022±0.008, p=ns). Ductal SGECs (n=4) isolated by laser microdissection of MSG revealed a higher expression of MAP1LC3 (0.005±0.0005 vs 0.003±0.0008; p=0.057) compared to normal acinar epithelium (n=5); a major expression of LC3II in ducts was confirmed by IFI (Image).In SS, a higher expression of ICAM compared to sicca was observed (11.1±3.8 vs 6.9±6.9, p=0.006) and autophagy and apoptosis showed a trend of positive and negative correlation with this molecule, respectively (p=0.683 r=0.118 and p=0.106 r=-0.446).Figure.LC3-II staining in SS MSG [LC3-II+ (green) and Hoechst stain (blue); 60x magnification].Conclusion:In SS, autophagy is upregulated in SGECs and inversely correlated with apoptosis, thus supporting a role of this process in cells’ death prevention during inflammatory process. Indeed, the degree of msg inflammation is correlated more with the activation of autophagy than apoptosis. Interesting, in SS, SGECs autophagy is mainly observed at ductal level and is correlated with higher expression of adhesion molecules suggesting a link between this pathway and changes in SGECs immune phenotype.Disclosure of Interests: :Serena Colafrancesco: None declared, cristiana barbati: None declared, Valentina Iannizzotto: None declared, Linda Mastromanno: None declared, Saba Nayar: None declared, Elena Pipi: None declared, angelica gattamelata: None declared, francesco ciccia Grant/research support from: pfizer, novartis, roche, Consultant of: pfizer, novartis, lilly, abbvie, Speakers bureau: pfizer, novartis, lilly, abbvie, cristiano alessandri Grant/research support from: Pfizer, Francesca Barone: None declared, fabrizio conti Speakers bureau: BMS, Lilly, Abbvie, Pfizer, Sanofi, Roberta Priori: None declared

Cell-specific epigenome-wide DNA methylation profile in long-term cultured minor salivary gland epithelial cells from patients with Sjögren's syndrome

2017 ◽  
Vol 76 (3) ◽  
pp. 625-628 ◽  
Author(s):  
Amandine Charras ◽  
Orsia D Konsta ◽  
Christelle Le Dantec ◽  
Cristina Bagacean ◽  
Efstathia K Kapsogeorgou ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Salivary Gland ◽  
Epithelial Cells ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Salivary Gland Epithelial Cells ◽  
Sjögren‘S Syndrome

ObjectivesThe aetiology of primary Sjögren's syndrome (pSS), also referred to as autoimmune epithelitis, is incompletely understood but includes an epigenetic contribution. Accordingly, the aim of this study was to investigate DNA methylation in salivary gland epithelial cells (SGEC), and to compare results with those publicly available from pSS B and T cells.MethodsLong-term cultured SGEC were selected to conduct an epigenome-wide association study (EWAS) in patients with pSS with comparison to controls using the HumanMethylation 450 K array from Illumina.ResultsThe analysis of differentially methylated CpG (DMC) uncovered 4662 positions corresponding to 2560 genes, and 575 genes with two or more DMC sites (DMCs), in SGEC as compared with controls. Further analysis highlighted an important proportion of interferon-regulated genes (61%), the calcium pathway (hypomethylated) and the Wnt pathway (hypermethylated). When comparing SGEC with pSS T and/or B cell results, an important overlap was observed with respect to differentially methylated genes (38.8%) and pSS risk factors (71.4%), although such assertion was not true when comparing DMCs.ConclusionsThis study conducted in SGEC emphasises the role of DNA methylation in pSS pathogenesis and supports the necessity to conduct pure cell analysis for future EWAS studies when analysing salivary glands from patients with pSS.

Sign in / Sign up

Export Citation Format

Share Document