scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation

scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2020 ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Iron Chelator ◽  
Infection Model ◽  
Invasive Infection ◽  
Liver Abscesses ◽  
Severe Infections ◽  
Lower Expression ◽  
Additional Iron

Abstract Background: Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. Results: All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P<0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions (P<0.05). The relative expression levels of the four siderophore genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions (P<0.05). Conclusions: It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses. A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomena is warranted.

scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2020 ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Iron Chelator ◽  
Infection Model ◽  
Invasive Infection ◽  
Liver Abscesses ◽  
Severe Infections ◽  
Lower Expression ◽  
Additional Iron

Abstract Background: Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. Results: All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P<0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions (P<0.05). The relative expression levels of the four siderophore genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions (P<0.05). Conclusions: It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses. A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomena is warranted.

scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2019 ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Iron Chelator ◽  
Infection Model ◽  
Invasive Infection ◽  
Iron Depletion ◽  
Liver Abscesses ◽  
Severe Infections ◽  
Additional Iron

Abstract Background: Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. Results: All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P<0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron depletion conditions (P<0.05). The relative expression levels of the four siderophore genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron depletion conditions (P<0.05). Conclusions: It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses. A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomena is warranted.

scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2020 ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Iron Chelator ◽  
Infection Model ◽  
Invasive Infection ◽  
Liver Abscesses ◽  
Severe Infections ◽  
Lower Expression ◽  
Additional Iron

Abstract Background: Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. Results: All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P<0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions (P<0.05). The relative expression levels of the four siderophore genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions (P<0.05). Conclusions: It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses. A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomena is warranted.

scholarly journals Isolation of Genes Involved in Biofilm Formation of a Klebsiella pneumoniae Strain Causing Pyogenic Liver Abscess

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23500 ◽  
Author(s):  
Meng-Chuan Wu ◽  
Tzu-Lung Lin ◽  
Pei-Fang Hsieh ◽  
Hui-Ching Yang ◽  
Jin-Town Wang
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Pyogenic Liver Abscess

scholarly journals Virulence Characteristics and Molecular Epidemiology of Pyogenic Liver Abscess Causing Multidrug Resistant Klebsiella pneumoniae in Wenzhou, China

2021 ◽  
Author(s):  
Zhongyong Wang ◽  
Siqin Zhang ◽  
Na Huang ◽  
Shixing Liu ◽  
Ye Xu ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Epidemiology ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Growth Curves ◽  
Pyogenic Liver Abscess ◽  
Multidrug Resistant ◽  
Infection Model ◽  
String Test ◽  
Serum Killing

Abstract Background To date, little is known about the virulence characteristics of pyogenic liver abscess (PLA) that cause multidrug resistant (MDR) Klebsiella pneumoniae (K. pneumoniae), which might be due to the rarity of these strains. This study aimed to analyze the virulence characteristics and molecular epidemiology of 12 MDR strains obtained from 163 PLA cases in a tertiary teaching hospital from the perspective of clinical characteristics, virulence phenotypes, and genotypes. Methods The MDR strains were obtained from sterile fluid samples collected from patients with PLA. The antimicrobial susceptibility testing was confirmed by the agar dilution method and microdilution broth method. The virulence phenotypes were analyzed by the growth curves, string test, capsular quantification, serum killing test, biofilm formation assay and infection model. Polymerase chain reaction (PCR) was used to investigate the virulence genotypes. The molecular epidemiology was identified by multilocus sequence typing (MLST). Results The results of growth curves, string test, capsular quantification, serum killing test, biofilm formation assay, and infection model revealed that the virulence phenotypes of the 12 PLA-causing MDR K. pneumoniae were similar to or more obvious than those of the typical hypervirulent K. pneumoniae strains. These MDR strains were mainly non-K1/K2 serotypes and carried multiple virulence genes. The results of MLST illustrated that the MDR strains were categorized into 9 sequence types. Conclusions This is the first study to analyze the virulence characteristics in PLA-causing MDR strains. The data revealed the coexistence of hypervirulence and MDR in PLA-causing MDR K. pneumoniae strains, and the clones of these strains were diverse and scattered. Also, one ST11 carbapenem-resistant hypervirulent strain was identified in PLA.

scholarly journals Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

2019 ◽  
Author(s):  
Tao Chen ◽  
Guofeng Dong ◽  
Siqin Zhang ◽  
Xiucai Zhang ◽  
Yajie Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Biofilm Formation ◽  
Iron Chelator ◽  
Infection Model ◽  
Relative Expression Level ◽  
Liver Abscesses ◽  
Severe Infections ◽  
Lower Expression ◽  
Additional Iron

Abstract Background: Klebsiella pneumoniae is considered as the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge,iron in human body primarily gathers in liver. Further understanding of effects of iron on K. pneumoniae causing liver abscess is a promising way to elucidate the mechanism of poor prognosis of liver abscess. Results: All tested K. pneumoniae including those collected from liver abscess and other sterile sites and K. pneumoniae ATCC 700603 cultured in LB broth containing 50 μM iron grew optimally and were in possession of the strongest biofilm formation ability, while worst growth and biofilm formation ability of the strains cultured in iron-deplete environment were observed. The infection model of G. mellonella larvae showed the virulence of K. pneumoniae cultured with additional iron was significantly higher than cultured with iron chelator (P<0.05). The relative expression level of the four siderophores genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscess cultured with additional iron was lower than that with iron chelator. Conclusions: It is suggested by our research that iron in the environment can promote the growth and enhance the biofilm formation ability of K. pneumoniae and the lower expression of siderophores genes may be one of the mechanisms of iron affecting the virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomenons is warranted.

Sign in / Sign up

Export Citation Format

Share Document