scholarly journals Laser capture microdissection for transcriptomic profiles in human skin biopsies

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Silvia Santoro ◽  
Ignazio Diego Lopez ◽  
Raffaella Lombardi ◽  
Andrea Zauli ◽  
Ana Maria Osiceanu ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Capture Microdissection ◽  
Skin Biopsies ◽  
Laser Capture

scholarly journals A novel technique for the examination of skin biopsies by laser capture microdissection

2003 ◽  
Vol 30 (4) ◽  
pp. 265-270 ◽  
Author(s):  
N. S. Agar ◽  
G. M. Halliday ◽  
R. St C. Barnetson ◽  
A. M. Jones
Keyword(s):  
Laser Capture Microdissection ◽  
Novel Technique ◽  
Skin Biopsies ◽  
Laser Capture

Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study

2006 ◽  
Vol 8 (3) ◽  
pp. 269-282 ◽  
Author(s):  
Katrin Lochner ◽  
Astrid Gaemlich ◽  
Kirstin Marie Südel ◽  
Kirsten Venzke ◽  
Ingrid Moll ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Capture Microdissection ◽  
Laser Capture

Changes in corneocyte element concentrations occur in skin inner stratum corneum

1990 ◽  
Vol 48 (2) ◽  
pp. 146-147
Author(s):  
R. R. Warner
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Element Concentration ◽  
Skin Barrier ◽  
Barrier Properties ◽  
Brick Wall ◽  
Inorganic Elements ◽  
Dry Skin ◽  
Skin Biopsies ◽  
Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

Isolating Human Notochord Using Laser Capture Microdissection

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Youssef Yakkioui ◽  
Remco Santegoeds ◽  
Koo van Overbeeke ◽  
Andreas Herrler ◽  
Yasin Temel
Keyword(s):  
Laser Capture Microdissection ◽  
Laser Capture

Cell‐Type‐Specific Proteomics Analysis of a Small Number of Plant Cells by Integrating Laser Capture Microdissection with a Nanodroplet Sample Processing Platform

2021 ◽  
Vol 1 (5) ◽  
Author(s):  
Vimal K. Balasubramanian ◽  
Samuel O. Purvine ◽  
Yiran Liang ◽  
Ryan T. Kelly ◽  
Ljiljana Pasa‐Tolic ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
Plant Cells ◽  
Cell Type ◽  
Sample Processing ◽  
Proteomics Analysis ◽  
Cell Type Specific ◽  
Processing Platform ◽  
Laser Capture

scholarly journals Stromal MED12 exon 2 mutations in complex fibroadenomas of the breast

2020 ◽  
pp. jclinpath-2020-207062
Author(s):  
Edaise M da Silva ◽  
Francisco Beca ◽  
Ana Paula Martins Sebastiao ◽  
Melissa P Murray ◽  
Catarina Silveira ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Laser Capture Microdissection ◽  
Sanger Sequencing ◽  
Mediator Complex ◽  
Telomerase Reverse Transcriptase ◽  
Exon 2 ◽  
Stromal Component ◽  
Hotspot Mutations ◽  
Laser Capture

AimsHere we explore the presence of mediator complex subunit 12 (MED12) exon 2 and telomerase reverse transcriptase (TERT) promoter hotspot mutations in complex fibroadenomas (CFAs) of the breast.MethodsThe stromal components from 18 CFAs were subjected to Sanger sequencing of MED12 exon 2 and the TERT promoter hotspot loci. The epithelial and stromal components of two MED12 mutated CFAs were subjected to laser capture microdissection, and Sanger sequencing of MED12 exon 2, TERT promoter and PIK3CA exons 9 and 20, separately.ResultsMED12 exon 2 mutations were identified in the stroma of 17% of CFAs. The analyses of epithelial and stromal components, microdissected separately, revealed that MED12 mutations were restricted to the stroma. No TERT promoter or PIK3CA mutations in exons 9 and 20 were detected in analysed CFAs.ConclusionsLike conventional fibroadenomas, MED12 exon 2 mutations appear to be restricted to the stromal component of CFAs, supporting the notion that CFAs are stromal neoplasms.

Sign in / Sign up

Export Citation Format

Share Document