scholarly journals Rapid diagnosis of Capnocytophaga canimorsus septic shock in an immunocompetent individual using real-time Nanopore sequencing: a case report

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Seweryn Bialasiewicz ◽  
Tania P. S. Duarte ◽  
Son H. Nguyen ◽  
Vichitra Sukumaran ◽  
Alexandra Stewart ◽  
...  
Author(s):  
Seweryn Bialasiewicz ◽  
Tania P.S. Duarte ◽  
Son H. Nguyen ◽  
Vichitra Sukumaran ◽  
Alexandra Stewart ◽  
...  

We present a case of Capnocytophaga canimorsus septic shock after a dog bite in an immunocompetent individual, where real-time nanopore metagenomic sequencing characterized the microbial agent within 19 hours, with subsequent confirmation using droplet digital PCR. Oral swabs from the dog demonstrated a nearly-identical C. canimorsus isolate by sequencing.


2021 ◽  
pp. 1-5
Author(s):  
Servet Duran ◽  
Dietske Miedema ◽  
Bülent Ergin ◽  
Can Ince

Cytokemia is associated with microcirculatory alterations often with persistent loss of coherence between the micro- and macrocirculation, linked to organ failure and poor outcome of septic patients. Addition of a hemoadsorbant filter to an extracorporeal circuit next to conventional treatment of septic shock results in the hematological clearance of cytokines, hypothetically leading to normalization of the microcirculation and thus organ perfusion. Bedside sublingual microcirculatory assessment using handheld vital microscopy allows real-time direct visualization of the microcirculation and its response to therapy. This is demonstrated in the present case report of an 83-year-old man admitted to our intensive care unit after surgical repair of a colonic perforation for fecal soiling after a low anterior resection for a rectum carcinoma, with leakage of bowel content at the resection site. The clinical course of this patient can be described as having undergone adequate surgical treatment taking away the source of the disease, followed by optimal support including antibiotic treatment in the ICU. However, during the course of his stay in the ICU, his condition deteriorated with symptoms consistent with septic shock. Our report shows that the addition of a hemoadsorbent (CytoSorb) to the continuous renal replacement therapy circuit was associated with an improvement in the condition of our severely ill patient with abdominal sepsis. Parallel to the clinical improvement of our patient, the functional parameters of the microcirculation also showed improvement suggesting that such a noninvasive real-time evaluation of the status of the microcirculation may be a sensitive diagnostic tool to monitor the effectiveness of hemoadsorbent therapy.


Author(s):  
Monica Torello ◽  
Stefania Valenti ◽  
Marco Rosetti ◽  
Vittorio Sambri ◽  
Romolo M. Dorizzi

2018 ◽  
Vol 72 ◽  
pp. 3-5 ◽  
Author(s):  
Giorgio Piccinelli ◽  
Francesca Caccuri ◽  
Elena De Peri ◽  
Andrea Tironi ◽  
Silvia Odolini ◽  
...  

1971 ◽  
Vol 36 (3) ◽  
pp. 397-409 ◽  
Author(s):  
Rachel E. Stark

Real-time amplitude contour and spectral displays were used in teaching speech production skills to a profoundly deaf, nonspeaking boy. This child had a visual attention problem, a behavior problem, and a poor academic record. In individual instruction, he was first taught to produce features of speech, for example, friction, nasal, and stop, which are present in vocalizations of 6- to 9-month-old infants, and then to combine these features in syllables and words. He made progress in speech, although sign language and finger spelling were taught at the same time. Speech production skills were retained after instruction was terminated. The results suggest that deaf children are able to extract information about the features of speech from visual displays, and that a developmental sequence should be followed as far as possible in teaching speech production skills to them.


2016 ◽  
Vol 7 (1) ◽  
pp. 61-64
Author(s):  
Urszula Zielińska-Borkowska ◽  
Aneta Słabuszewska-Joźwiak

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