scholarly journals Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Peter Schotte ◽  
Isabelle Dewerte ◽  
Manu De Groeve ◽  
Saskia De Keyser ◽  
Veronique De Brabandere ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Methionine Residues

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

Pichia pastoris fermentation for phytase production using crude glycerol from biodiesel production as the sole carbon source

2009 ◽  
Vol 43 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Shuiquan Tang ◽  
Lindsay Boehme ◽  
Howard Lam ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Crude Glycerol ◽  
Biodiesel Production ◽  
Phytase Production

Deletion of the GCW13 gene derepresses Gap1-dependent uptake of amino acids in Pichia pastoris grown on methanol as the sole carbon source

2018 ◽  
Vol 501 (1) ◽  
pp. 226-231 ◽  
Author(s):  
Chengjuan Zou ◽  
Pan Wang ◽  
Shuli Liang ◽  
Shuangyan Han ◽  
Suiping Zheng ◽  
...  
Keyword(s):  
Amino Acids ◽  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source

scholarly journals Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121 ◽  
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

High level expression of a recombinant xylanase by Pichia pastoris cultured in a bioreactor with methanol as the sole carbon source: Purification and biochemical characterization of the enzyme

2016 ◽  
Vol 112 ◽  
pp. 161-169 ◽  
Author(s):  
Maribel Cayetano-Cruz ◽  
Ara Itzel Pérez de los Santos ◽  
Yolanda García-Huante ◽  
Alejandro Santiago-Hernández ◽  
Patricia Pavón-Orozco ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Biochemical Characterization ◽  
High Level Expression ◽  
High Level

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

Performance enhancement of H2S-based autotrophic denitrification with bio-gaseous CO2 as sole carbon source through new pH adjustment materials

2020 ◽  
Vol 261 ◽  
pp. 110157 ◽  
Author(s):  
Yongjie Liu ◽  
Nan Chen ◽  
Shuang Tong ◽  
Jing Liang ◽  
Chen Yang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Performance Enhancement ◽  
Autotrophic Denitrification ◽  
Ph Adjustment

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium
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