Deletion of the GCW13 gene derepresses Gap1-dependent uptake of amino acids in Pichia pastoris grown on methanol as the sole carbon source

2018 ◽  
Vol 501 (1) ◽  
pp. 226-231 ◽  
Author(s):  
Chengjuan Zou ◽  
Pan Wang ◽  
Shuli Liang ◽  
Shuangyan Han ◽  
Suiping Zheng ◽  
...  
Keyword(s):  
Amino Acids ◽  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

scholarly journals Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Peter Schotte ◽  
Isabelle Dewerte ◽  
Manu De Groeve ◽  
Saskia De Keyser ◽  
Veronique De Brabandere ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Methionine Residues

The effect of carbon source on trap formation by Arthrobotrys conoides

1968 ◽  
Vol 14 (11) ◽  
pp. 1260-1261
Author(s):  
A. W. James ◽  
R. J. Nowakowski
Keyword(s):  
Amino Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Major Effect ◽  
Trap Formation ◽  
Arthrobotrys Conoides

The nematode-trapping fungus Arthrobotrys conoides grows as typical vegetative hyphae on laboratory media containing glucose or starch as sole carbon source. However, in the presence of nematodes, "nemin", and several of the aliphatic amino acids (such as valine), this predacious fungus forms traps which are composed of sticky loops. The present studies show that, when A. conoides is grown on a semisynthetic medium in which various carbohydrates are substituted for glucose as the carbon source available for growth, the nature of the carbohydrate exerts a major effect on the induction of trap formation.

scholarly journals Constructing an ethanol utilization pathway in Escherichia coli to produce acetyl-CoA derived compounds

2020 ◽  
Author(s):  
Hong Liang ◽  
Xiaoqiang Ma ◽  
Wenbo Ning ◽  
Yurou Liu ◽  
Anthony J. Sinskey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Structural Similarity ◽  
List Type ◽  
Acetyl Coa ◽  
E Coli ◽  
Utilization Pathway ◽  
Aspartate Family

AbstractEngineering microbes to utilize non-conventional substrates could create short and efficient pathways to convert substrate into product. In this study, we designed and constructed a two-step heterologous ethanol utilization pathway (EUP) in Escherichia coli by using acetaldehyde dehydrogenase (encoded by ada) from Dickeya zeae and alcohol dehydrogenase (encoded by adh2) from Saccharomyces cerevisiae. This EUP can convert ethanol into acetyl-CoA without ATP consumption, and generate two molecules of NADH per molecule of ethanol. We optimized the expression of these two genes and found that ethanol consumption could be improved by expressing them in a specific order (ada-adh2) with a constitutive promoter (PgyrA). The engineered E. coli strain with EUP consumed approximately 8 g/L of ethanol in 96 hours when it was used as sole carbon source. Subsequently, we combined EUP with the biosynthesis of polyhydroxybutyrate (PHB), a biodegradable polymer derived from acetyl-CoA. The engineered E. coli strain carrying EUP and PHB biosynthetic pathway produced 1.1 g/L of PHB from 10 g/L of ethanol and 1 g/L of aspartate family amino acids in 96 hours. We also engineered E. coli strain to produced 24 mg/L of prenol from 10 g/L of ethanol in 48 hours, supporting the feasibility of converting ethanol into different classes of acetyl-CoA derived compounds.HighlightsEngineered Escherichia coli strains to grow on ethanol as sole carbon sourceDemonstrated that ethanol was converted into acetyl-CoA (AcCoA) through two pathways (acetaldehyde-acetate-AcCoA and acetaldehyde-AcCoA)Converted ethanol into two acetyl-CoA derived products with low structural similarity (polyhydroxybutyrate and prenol)Discovered that supplementation of the aspartate family amino acids can substantially improve cell growth on ethanol

scholarly journals Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121 ◽  
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

High level expression of a recombinant xylanase by Pichia pastoris cultured in a bioreactor with methanol as the sole carbon source: Purification and biochemical characterization of the enzyme

2016 ◽  
Vol 112 ◽  
pp. 161-169 ◽  
Author(s):  
Maribel Cayetano-Cruz ◽  
Ara Itzel Pérez de los Santos ◽  
Yolanda García-Huante ◽  
Alejandro Santiago-Hernández ◽  
Patricia Pavón-Orozco ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Biochemical Characterization ◽  
High Level Expression ◽  
High Level

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp
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