scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium

scholarly journals Extra-Heavy Crude Oil Degradation by Alternaria sp. Isolated from Deep-Sea Sediments of the Gulf of Mexico

2021 ◽  
Vol 11 (13) ◽  
pp. 6090
Author(s):  
Lucia Romero-Hernández ◽  
Patricia Velez ◽  
Itandehui Betanzo-Gutiérrez ◽  
María Dolores Camacho-López ◽  
Rafael Vázquez-Duhalt ◽  
...  
Keyword(s):  
Carbon Source ◽  
Gulf Of Mexico ◽  
Crude Oil ◽  
Deep Sea ◽  
Sole Carbon Source ◽  
The United States ◽  
Heavy Crude Oil ◽  
Deep Sea Sediments ◽  
Alternaria Sp ◽  
Heavy Crude

The Gulf of Mexico (GoM) is an important source of oil for the United States and Mexico. There has been growing interest, particularly after the Deepwater Horizon oil spill, in characterizing the fungal diversity of the GoM and identifying isolates for use in the bioremediation of petroleum in the event of another spill. Most studies have focused on light crude oil bioremediation processes, while heavy crude oil (HCO) and extra-heavy crude oil (EHCO) have been largely ignored. In this work, we evaluated the ability of fungal isolates obtained from deep-sea sediments of the Mexican economic exclusive zone (EEZ) of the GoM to degrade HCO (16–20° API) and EHCO (7–10° API). Alternaria sp., Penicillium spp., and Stemphylium sp. grew with HCO as the sole carbon source. Remarkably, Alternaria sp. was the only isolate able to grow with EHCO as the sole carbon source, degrading up to 25.6% of the total EHCO and 91.3% of the aromatic fraction, as demonstrated by gas chromatography analysis of the saturate, aromatic, and polar fractions. These findings proved to be significant, identifying Alternaria sp. as one of the few fungi reported so far capable of degrading untreated EHCO and as a suitable candidate for bioremediation of EHCO in future studies.

Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria

1983 ◽  
Vol 29 (5) ◽  
pp. 497-503 ◽  
Author(s):  
P. M. Fedorak ◽  
D. W. S. Westlake
Keyword(s):  
Carbon Source ◽  
Benzoic Acid ◽  
Crude Oil ◽  
Marine Sediment ◽  
Marine Bacteria ◽  
Sole Carbon Source ◽  
Bacterial Isolates ◽  
Aromatic Fraction ◽  
Selective Degradation ◽  
Prudhoe Bay

Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degraded only biphenyl, 3-methylbiphenyl, 4-methylbiphenyl, and three other minor, unidentified compounds in the aromatic fraction. No degradation was detected in the saturate fraction, nor in the sulfur heterocyclic component of the oil. When grown on any of the pure biphenyls, these isolates produced a transient, yellow intermediate which had the spectrophotometric characteristics of α-hydroxymuconic semialdehydes. Growth on either methylbiphenyl compound produced a methylbenzoic acid, indicating that the nonmethylated ring was the first to be cleaved. The isolates, identified as Alcaligenes sp. and Acinetobacter sp., were also able to grow on benzoic acid, 3-, and 4-methylbenzoic acids, indicating that they could further metabolize the aromatic acid intermediates.

The role of interactions, sessile growth, and nutrient amendments on the degradative efficiency of a microbial consortium

1994 ◽  
Vol 40 (5) ◽  
pp. 331-340 ◽  
Author(s):  
G. M. Wolfaardt ◽  
J. R. Lawrence ◽  
R. D. Robarts ◽  
D. E. Caldwell
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Spatial Organization ◽  
Microbial Consortium ◽  
Algal Species ◽  
Algal Growth ◽  
Bacterial Consortium ◽  
Microbial Interactions ◽  
Significant Difference ◽  
Pure Cultures

A degradative microbial consortium consisting of at least nine bacterial and one algal species was isolated from soil with diclofop methyl as the sole carbon source. In continuous flow culture, the presence of the algae increased diclofop methyl degradation and removal by 36%. Batch culture experiments with 14C-labeled diclofop methyl confirmed algal involvement in the mineralization of diclofop methyl as there was no significant difference in the amount of 14CO2 evolved by the bacterial consortium with and without the algal activity when the consortium was cultivated in the dark to inhibit algal growth, while 11% more 14CO2 was produced in the light by the algal–bacterial consortium. Pure cultures isolated from the bacterial consortium could not individually mineralize diclofop methyl as the sole carbon source. However, when supplied with an additional carbon source, two strains could mineralize diclofop methyl. Addition of either the complex growth medium, or a cell-free filtrate from the algal–bacterial consortium to batch systems containing 14C-labeled diclofop methyl resulted in a significant increase in the production of 14CO2 by the bacterial consortium, suggesting co-metabolism of diclofop methyl in the presence of a labile carbon source. Removal of diclofop methyl by the bacterial consortium was increased by 36% when a larger surface to volume ratio was provided by glass beads that allowed extensive biofilm formation. The requirement for exogenous carbon sources and the inability of isolated pure cultures to degrade diclofop methyl indicated that interspecies interactions are necessary for degradation. The positive effect of sessile growth suggested that spatial organization of cells may also be important for degradation.Key words: consortium, degradation, herbicide, microbial interactions.

scholarly journals Biofilm-Mediated Enhanced Crude Oil Degradation by Newly Isolated Pseudomonas Species

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Debdeep Dasgupta ◽  
Ritabrata Ghosh ◽  
Tapas K. Sengupta
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
River Estuary ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Oil Degradation ◽  
Topographic Analysis ◽  
Degrading Bacteria ◽  
Crude Oil Degradation

The bioavailability of organic contaminants to the degrading bacteria is a major limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants. Such limitation of bioavailability can be overcome by steady-state biofilm-based reactor. The aim of this study was to examine the effect of such multicellular aggregation by naturally existing oil-degrading bacteria on crude oil degradation. Microorganisms, capable of utilizing crude oil as sole carbon source, were isolated from river, estuary and sea-water samples. Biochemical and 16S rDNA analysis of the best degraders of the three sources was found to belong to the Pseudomonas species. Interestingly, one of the isolates was found to be close to Pseudomonas otitidis family which is not reported yet as a degrader of crude oil. Biodegradation of crude oil was estimated by gas chromatography, and biofilm formation near oil-water interface was quantified by confocal laser scanning microscopy. Biofilm supported batches of the isolated Pseudomonas species were able to degrade crude oil much readily and extensively than the planktonic counterparts. Volumetric and topographic analysis revealed that biofilms formed in presence of crude oil accumulate higher biomass with greater thickness compared to the biofilms produced in presence of glucose as sole carbon source.

Characterization of crude oil degrading microbial cultures isolated in Qingdao China

RSC Advances ◽  
2015 ◽  
Vol 5 (118) ◽  
pp. 97665-97674 ◽  
Author(s):  
Yongrui Pi ◽  
Mutai Bao ◽  
Yiming Li ◽  
Guangmei Li ◽  
Jinren Lu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Sediment Samples ◽  
Microbial Cultures ◽  
Water And Sediment ◽  
Water And Sediment Samples

9 hydrocarbon-degrading strains were isolated based on their ability to grow with crude oil as the sole carbon source from the water and sediment samples of Qingdao offshore.

scholarly journals Complete Genome Sequence of the Carotenoid-Producing Strain Gordonia ajoucoccus A2

2020 ◽  
Vol 9 (37) ◽  
Author(s):  
Soon Jae Kwon ◽  
Yong Jin Choi ◽  
Ju Min Kim ◽  
Pyung Cheon Lee
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Contaminated Soils ◽  
Complete Genome ◽  
Sole Carbon Source ◽  
Circular Chromosome ◽  
Content Type ◽  
Single Circular Chromosome

ABSTRACT Gordonia ajoucoccus strain A2, isolated from crude oil-contaminated soils, synthesizes yellow keto-γ-carotene from various n-alkanes as the sole carbon source. Its complete genome sequence consists of a single circular chromosome (5,090,254 bp, 67.3% G+C content). Seven putative genes were identified supporting the proposed keto-γ-carotene pathway of G. ajoucoccus A2.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp
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