scholarly journals Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
A. Zerva ◽  
C. Pentari ◽  
A. Termentzi ◽  
A. H. P. America ◽  
D. Zouraris ◽  
...  
Keyword(s):  
Redox Potential ◽  
Sinapic Acid ◽  
Physiological Role ◽  
White Rot Fungi ◽  
White Rot ◽  
Multicopper Oxidases ◽  
Laccase Genes ◽  
Pleurotus Citrinopileatus

Abstract Background Laccases and laccase-like multicopper oxidases (LMCOs) oxidize a vast array of phenolic compounds and amines, releasing water as a byproduct. Their low substrate specificity is responsible for their tremendous biotechnological interest, since they have been used for numerous applications. However, the laccases characterized so far correspond to only a small fraction of the laccase genes identified in fungal genomes. Therefore, the knowledge regarding the biochemistry and physiological role of minor laccase-like isoforms is still limited. Results In the present work, we describe the isolation, purification and characterization of two novel LMCOs, PcLac1 and PcLac2, from Pleurotus citrinopileatus. Both LMCOs were purified with ion-exchange chromatographic methods. PcLac2 was found to oxidize a broader substrate range than PcLac1, but both LMCOs showed similar formal potentials, lower than those reported previously for laccases from white-rot fungi. Proteomic analysis of both proteins revealed their similarity with other well-characterized laccases from Pleurotus strains. Both LMCOs were applied to the oxidation of ferulic and sinapic acid, yielding oligomers with possible antioxidant activity. Conclusions Overall, the findings of the present work can offer new insights regarding the biochemistry and variability of low-redox potential laccases of fungal origin. Low-redox potential biocatalysts could offer higher substrate selectivity than their high-redox counterparts, and thus, they could be of applied value in the field of biocatalysis.

Characterization of three multicopper oxidases in the filamentous fungus Podospora anserina : A new role of an ABR1-like protein in fungal development?

2018 ◽  
Vol 116 ◽  
pp. 1-13 ◽  
Author(s):  
Ning Xie ◽  
Gwenaël Ruprich-Robert ◽  
Philippe Silar ◽  
Eric Herbert ◽  
Roselyne Ferrari ◽  
...  
Keyword(s):  
Filamentous Fungus ◽  
Podospora Anserina ◽  
Multicopper Oxidases ◽  
Fungal Development

scholarly journals The attraction of Tremex apicalis (Hymenoptera, Siricidae, Tremecinae) and its parasitoid Ibalia japonica (Hymenoptera, Ibaliidae) to the fungus Cerrena unicolor

2019 ◽  
Vol 68 ◽  
pp. 37-48 ◽  
Author(s):  
Kazumu Kuramitsu ◽  
Teruhito Ishihara ◽  
Aki Sugita ◽  
Thitaree Yooboon ◽  
Barry Lustig ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Dna Barcode ◽  
White Rot Fungi ◽  
White Rot ◽  
Searching Behavior ◽  
Host Searching ◽  
Additional Species ◽  
Saproxylic Insects ◽  
Symbiotic Fungi

Woodwasps (Hymenoptera: Siricidae) are saproxylic insects and a common forest pest. Siricid woodwasps are classified into two subfamilies: Siricinae and Tremecinae. All known symbiotic fungi of Siricinae are in the genusAmylostereumBoidin while some species of Tremecinae have been observed to have a relationship with the fungusCerrenaunicolor(Bull.) Murrill. Previous studies about the host searching behavior of woodwasps and their parasitoids have focused primarily on the subfamily Siricinae.We analyzed the role ofC.unicolorvolatiles on the host searching behavior ofTremexapicalisMatsumura (Hymenoptera: Siricidae: Tremecinae) and its parasitoid Ibalia (Tremibalia) japonica Matsumura (Hymenoptera: Ibaliidae). The results of an olfactory response experiment indicated that the females ofT.apicalisand its parasitoid find their respective hosts using volatiles fromC.unicolor. Using DNA barcode, we identified basidiocarps on the trees infested withT.apicalis.The basidiocarps were all white-rot fungi that cause sapwood decay, includingC.unicolor. Two additional species that we identified belonged to genera closely related toC.unicolor.Woodwasp species are known to carry symbiotic fungi in a pair of specialized sacs called mycangia. Notably we found that mycangia-like structures were absent in the abdomens ofT.apicalisfemales. To the best of our knowledge,Xerisspectrum(Linnaeus) (Hymenoptera: Siricidae) is the only reported example of woodwasp species that do not contain symbiotic fungi in their bodies.Our results suggested that: (1)T.apicalisfemales search for host wood that is already infected with sapwood decaying fungus using volatile compounds; (2)T.apicalis’ female parasitoid also uses volatile compounds from fungus to locate wood that is infested with its potential host.

Biodegradation of Lignocellulose by White-Rot Fungi: Structural Characterization of Water-Soluble Hemicelluloses

2013 ◽  
Vol 6 (4) ◽  
pp. 1154-1164 ◽  
Author(s):  
Ling-Ping Xiao ◽  
Zheng-Jun Shi ◽  
Yuan-Yuan Bai ◽  
Wei Wang ◽  
Xue-Ming Zhang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
White Rot Fungi ◽  
Water Soluble ◽  
White Rot

Characterization of an amphimull under Mediterranean evergreen oak forest (Quercus ilex): micromorphological and biodynamic descriptions

2008 ◽  
Vol 38 (2) ◽  
pp. 268-277 ◽  
Author(s):  
Simone Tagger ◽  
Claude Périssol ◽  
Stéven Criquet ◽  
Guy Aubert ◽  
Pierre Neville ◽  
...  
Keyword(s):  
Quercus Ilex ◽  
White Rot Fungi ◽  
White Rot ◽  
Available Phosphorus ◽  
Calcium Oxalate Crystals ◽  
Litter Degradation ◽  
Evergreen Oak Forest ◽  
Evergreen Oak ◽  
Microbiological Properties

The relationships between microorganisms and microfauna were studied in an evergreen oak ( Quercus ilex L.) forest floor located in a French Mediterranean region characterized by hot and dry summers. The soil was a brown fersialitic soil with an amphimull, i.e., a mull with a thick litter. A micromorphological study of both litter and organomineral layers was used to observe relationships between white-rot fungi and fauna, such as oribatids, millipedes, enchytraeids, and earthworms. Microbiological properties of the litter and physicochemical properties of the soil were analysed. Enchytraeids comminute the faeces of other animals, whereas earthworms form aggregates. These two opposite actions probably modify aeration and water movements in deeper layers. Brown leaves and animal faeces constitute a nutritional substrate for white-rot fungi and other animals. Inside degraded cells of rootlets and bleached leaves, fungi form calcium oxalate crystals because the absorbent complex is saturated by an excess of calcium. Enzymes such as laccases, manganese peroxidases, cellulases, or xylanases were detected in the litter at significant levels. The high activity values of phosphatases in the litter could reflect a deficiency in available phosphorus. This deficiency could partly explain a low rate of litter degradation and the presence of an OH sublayer.

scholarly journals Use of Multiplex Reverse Transcription-PCR To Study the Expression of a Laccase Gene Family in a Basidiomycetous Fungus

2003 ◽  
Vol 69 (12) ◽  
pp. 7083-7090 ◽  
Author(s):  
Tania González ◽  
María C. Terrón ◽  
Ernesto J. Zapico ◽  
Alejandro Téllez ◽  
Susana Yagüe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reverse Transcription ◽  
White Rot Fungi ◽  
Gene Families ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Laccase Gene ◽  
Reverse Transcription Pcr ◽  
Basidiomycetous Fungus ◽  
Laccase Genes

ABSTRACT Laccases produced by white rot fungi are involved in the degradation of lignin and a broad diversity of other natural and synthetic molecules, having a great potential for biotechnological applications. They are frequently encoded by gene families, as in the basidiomycete Trametes sp. strain I-62, from which the lcc1, lcc2, and lcc3 laccase genes have been cloned and sequenced. A multiplex reverse transcription-PCR method to simultaneously study the expression of these genes was developed in this study. The assay proved to be quick, simple, highly sensitive, and reproducible and is particularly valuable when numerous samples are to be analyzed and/or if the amount of initial mRNA is limited. It was used to analyze the effect of 3,4-dimethoxybenzyl alcohol (veratryl alcohol) and two of its isomers (2,5-dimethoxybenzyl alcohol and 3,5-dimethoxybenzyl alcohol) on differential laccase gene expression in Trametes sp. strain I-62. These aromatic compounds produced different induction patterns despite their chemical similarity. We found 2,5-dimethoxybenzyl alcohol to be the best inducer of laccase activity while also producing the highest increase in gene expression; 3,5-dimethoxybenzyl alcohol was the next best inducer. Transcript amounts of each gene fluctuated dramatically in the presence of these three inducers, while the total amounts of laccase mRNAs seemed to be modulated by a coordinated regulation of the different genes.

Characterization of acetylated wood decayed by brown-rot and white-rot fungi

1999 ◽  
Vol 45 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Makoto Ohkoshi ◽  
Atsushi Kato ◽  
Kentaro Suzuki ◽  
Noriko Hayashi ◽  
Mitsuro Ishihara
Keyword(s):  
White Rot Fungi ◽  
Brown Rot ◽  
White Rot
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