Improvement of Methods for the Isolation of Cell-Free Fetal DNA from Maternal Plasma: Comparison of a Manual and an Automated Method

2006 ◽  
Vol 1075 (1) ◽  
pp. 308-312 ◽  
Author(s):  
D. J HUANG ◽  
B. G ZIMMERMANN ◽  
W. HOLZGREVE ◽  
S. HAHN
Keyword(s):  
Maternal Plasma ◽  
Fetal Dna ◽  
Automated Method ◽  
Cell Free Fetal Dna

scholarly journals Use of an Automated Method Improves the Yield and Quality of Cell-Free Fetal DNA Extracted from Maternal Plasma

2005 ◽  
Vol 51 (12) ◽  
pp. 2419-2420 ◽  
Author(s):  
Dorothy J Huang ◽  
Bernhard G Zimmermann ◽  
Wolfgang Holzgreve ◽  
Sinuhe Hahn
Keyword(s):  
Maternal Plasma ◽  
Fetal Dna ◽  
Automated Method ◽  
Yield And Quality ◽  
Cell Free Fetal Dna

Placental volume and cell-free fetal DNA levels in maternal plasma between 15 and 18 gestational weeks

2006 ◽  
Vol 195 (6) ◽  
pp. S174
Author(s):  
Donghyun Cha ◽  
Kyoungjin Lee ◽  
Ji Hyun Park ◽  
Kyoung Joo Lee ◽  
Ji Yeon Kim ◽  
...  
Keyword(s):  
Maternal Plasma ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Placental Volume

Cell-free Fetal DNA Analysis in Maternal Plasma as Screening Test for Trisomies 21, 18, and 13 in Twin Pregnancy

2019 ◽  
Vol 74 (2) ◽  
pp. 61-63
Author(s):  
G. Le Conte ◽  
A. Letourneau ◽  
J. Jani ◽  
P. Kleinfinger ◽  
L. Lohmann ◽  
...  
Keyword(s):  
Screening Test ◽  
Twin Pregnancy ◽  
Dna Analysis ◽  
Maternal Plasma ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Microsystem for Isolation of Fetal DNA from Maternal Plasma by Preparative Size Separation

2009 ◽  
Vol 55 (12) ◽  
pp. 2144-2152 ◽  
Author(s):  
Thomas Hahn ◽  
Klaus S Drese ◽  
Ciara K O'Sullivan
Keyword(s):  
Prenatal Diagnosis ◽  
High Volume ◽  
Maternal Plasma ◽  
Chromosomal Anomalies ◽  
Size Separation ◽  
Large Samples ◽  
Fetal Dna ◽  
Noninvasive Prenatal Diagnosis ◽  
Cell Free Fetal Dna ◽  
Invasive Techniques

Abstract Background: Routine prenatal diagnosis of chromosomal anomalies is based on invasive procedures, which carry a risk of approximately 1%–2% for loss of pregnancy. An alternative to these inherently invasive techniques is to isolate fetal DNA circulating in the pregnant mother’s plasma. Free fetal DNA circulates in maternal plasma primarily as fragments of lengths <500 bp, with a majority being <300 bp. Separating these fragments by size facilitates an increase in the ratio of fetal to maternal DNA. Methods: We describe our development of a microsystem for the enrichment and isolation of cell-free fetal DNA from maternal plasma. The first step involves a high-volume extraction from large samples of maternal plasma. The resulting 80-μL eluate is introduced into a polymeric microsystem within which DNA is trapped and preconcentrated. This step is followed by a transient isotachophoresis step in which the sample stacks within a neighboring channel for subsequent size separation and is recovered via an outlet at the end of the channel. Results: Recovered fractions of fetal DNA were concentrated 4–8 times over those in preconcentration samples. With plasma samples from pregnant women, we detected the fetal SRY gene (sex determining region Y) exclusively in the fragment fraction of <500 bp, whereas a LEP gene (leptin) fragment was detected in both the shorter and longer recovery fractions. Conclusions: The microdevice we have described has the potential to open new perspectives in noninvasive prenatal diagnosis by facilitating the isolation of fetal DNA from maternal plasma in an integrated, inexpensive, and easy-to-use microsystem.

scholarly journals Non-Invasive Prenatal RHD Genotyping Using Cell-Free Fetal DNA from Maternal Plasma: An Italian Experience

2014 ◽  
Vol 42 (1) ◽  
pp. 22-28 ◽  
Author(s):  
Elena Picchiassi ◽  
Gian Carlo Di Renzo ◽  
Federica Tarquini ◽  
Vittorio Bini ◽  
Michela Centra ◽  
...  
Keyword(s):  
Maternal Plasma ◽  
Non Invasive ◽  
Fetal Dna ◽  
Italian Experience ◽  
Cell Free Fetal Dna

Maternal smoking does not affect the amount of cell-free fetal DNA in maternal plasma during the 1st trimester of pregnancy

2014 ◽  
Vol 35 (1) ◽  
pp. 42-45 ◽  
Author(s):  
F. Tarquini ◽  
E. Picchiassi ◽  
M. Centra ◽  
L. Pennacchi ◽  
F. Galeone ◽  
...  
Keyword(s):  
Maternal Smoking ◽  
Maternal Plasma ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Noninvasive Prenatal Testing Using Cell‐Free Fetal DNA in Maternal Plasma

2015 ◽  
Vol 84 (1) ◽  
Author(s):  
Nilesh Dharajiya ◽  
Tricia Zwiefelhofer ◽  
Xiaojun Guan ◽  
Vach Angkachatchai ◽  
Juan‐Sebastian Saldivar
Keyword(s):  
Prenatal Testing ◽  
Maternal Plasma ◽  
Noninvasive Prenatal Testing ◽  
Fetal Dna ◽  
Cell Free Fetal Dna
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