Metabolism and Biotransformation of Xenobiotics

2021 ◽  
pp. 41-69
Author(s):  
Hilmi Orhan
Keyword(s):  
Biotransformation Of Xenobiotics

scholarly journals In situ proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals

2018 ◽  
Vol 74 (5) ◽  
pp. 300-306 ◽  
Author(s):  
Jovana J. Plavša ◽  
Pavlína Řezáčová ◽  
Michael Kugler ◽  
Petr Pachl ◽  
Jiří Brynda ◽  
...  
Keyword(s):  
High Resolution ◽  
Enzyme Inhibitor ◽  
Structural Studies ◽  
Potential Therapeutic Target ◽  
Thrombin Cleavage ◽  
His Tag ◽  
Structure Solution ◽  
Biotransformation Of Xenobiotics

Human aldo-keto reductase 1C3 (AKR1C3) stereospecifically reduces steroids and prostaglandins and is involved in the biotransformation of xenobiotics. Its role in various cancers makes it a potential therapeutic target for the development of inhibitors. Recombinant AKR1C3 with a thrombin-cleavable N-terminal His6 tag was expressed from a pET-28(+) vector for structural studies of enzyme–inhibitor complexes. A modified in situ proteolysis approach was applied to specifically remove the His tag by thrombin cleavage during crystallization screening trials. This improved the morphology and diffraction quality of the crystals and allowed the acquisition of high-resolution diffraction data and structure solution. This approach may be generally applicable to other proteins expressed using the pET-28(+) vector.

Detoxification and Biotransformation of Xenobiotics

2016 ◽  
pp. 525-525
Author(s):  
DM Vasudevan ◽  
Sreekumari S ◽  
Kannan Vaidyanathan
Keyword(s):  
Biotransformation Of Xenobiotics

scholarly journals Biotransformation of xenobiotics in human intestinal mucosa

1979 ◽  
Vol 33 ◽  
pp. 71-78 ◽  
Author(s):  
H. P. Hoensch ◽  
R. Hutt ◽  
F. Hartmann
Keyword(s):  
Intestinal Mucosa ◽  
Biotransformation Of Xenobiotics

Cytochrome-P450-Dependent Biotransformation of Xenobiotics in Human and Rodent Embryonic Tissues

1998 ◽  
Vol 30 (3) ◽  
pp. 541-568 ◽  
Author(s):  
Mont R. Juchau ◽  
Hélène Boutelet-Bochan ◽  
Ying Huang
Keyword(s):  
Cytochrome P450 ◽  
Embryonic Tissues ◽  
Biotransformation Of Xenobiotics

Reductive biotransformation of xenobiotics by the sheep ruminal content

1992 ◽  
Vol 101 (3) ◽  
pp. 625-626 ◽  
Author(s):  
O.Acosta de Pérez ◽  
A.S. Bernacchi ◽  
E.G.Díaz de Toranzo ◽  
J.A. Castro
Keyword(s):  
Biotransformation Of Xenobiotics

Gene polymorphisms of enzymes biotransformation of xenobiotics in patients with depressive disorders

2017 ◽  
Vol 27 ◽  
pp. S800-S801 ◽  
Author(s):  
L. Levchuk ◽  
I.S. Losenkov ◽  
E.V. Lebedeva ◽  
E.D. Schastnyy ◽  
O.E. Perchatkina ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Depressive Disorders ◽  
Biotransformation Of Xenobiotics

Microbial genetic potential for xenobiotic metabolism increases with depth during biofiltration

2020 ◽  
Vol 22 (10) ◽  
pp. 2058-2069
Author(s):  
Dong Li ◽  
Jonathan O. Sharp ◽  
Jörg E. Drewes
Keyword(s):  
Antibiotic Resistance ◽  
Xenobiotic Metabolism ◽  
Genetic Potential ◽  
Biotransformation Of Xenobiotics

Microbial genetic potential for the biotransformation of xenobiotics and antibiotic resistance increases with depth during biofiltration.

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