dual role
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2022 ◽  
Vol 807 ◽  
pp. 150670
Ximena Huérfano ◽  
José M. Estavillo ◽  
Fernando Torralbo ◽  
Izargi Vega-Mas ◽  
Carmen González-Murua ◽  

Cytokine ◽  
2022 ◽  
Vol 150 ◽  
pp. 155760
Yanglin Zhu ◽  
Hong Qin ◽  
Kui Ye ◽  
Chenglu Sun ◽  
Yafei Qin ◽  

Biomolecules ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 126
Rosa Fontana ◽  
Daniela Guidone ◽  
Tiziana Angrisano ◽  
Viola Calabrò ◽  
Alessandra Pollice ◽  

Background: The ARF tumour suppressor plays a well-established role as a tumour suppressor, halting cell growth by both p53-dependent and independent pathways in several cellular stress response circuits. However, data collected in recent years challenged the traditional role of this protein as a tumour suppressor. Cancer cells expressing high ARF levels showed that its expression, far from being dispensable, is required to guarantee tumour cell survival. In particular, ARF can promote autophagy, a self-digestion pathway that helps cells cope with stressful growth conditions arising during both physiological and pathological processes. Methods: We previously showed that ARF is regulated through the activation of the protein kinase C (PKC)-dependent pathway and that an ARF phospho-mimetic mutant on the threonine residue 8, ARF-T8D, sustains cell proliferation in HeLa cells. We now explored the role of ARF phosphorylation in both basal and starvation-induced autophagy by analysing autophagic flux in cells transfected with either WT and ARF phosphorylation mutants by immunoblot and immunofluorescence. Results: Here, we show that endogenous ARF expression in HeLa cells is required for starvation-induced autophagy. Further, we provide evidence that the hyper-expression of ARF-T8D appears to inhibit autophagy in both HeLa and lung cancer cells H1299. This effect is due to the cells’ inability to elicit autophagosomes formation upon T8D expression. Conclusions: Our results lead to the hypothesis that ARF phosphorylation could be a mechanism through which the protein promotes or counteracts autophagy. Several observations underline how autophagy could serve a dual role in cancer progression, either protecting healthy cells from damage or aiding cancerous cells to survive. Our results indicate that ARF phosphorylation controls protein’s ability to promote or counteract autophagy, providing evidence of the dual role played by ARF in cancer progression.

2022 ◽  
pp. 2104605
Liping Wang ◽  
Piotr Jankowski ◽  
Christian Njel ◽  
Werner Bauer ◽  
Zhenyou Li ◽  

2022 ◽  
pp. 002087282110657
Rae Morris ◽  
Celeste Borja

This Voices from Practice article shares reflections of two social workers on our dual-role experiences when conducting qualitative health research as trained clinicians. Reflections and considerations are shared for health care social work research trainees to prepare for and navigate the integration of the clinician-researcher role in social work.

Sivaparwathi Golla ◽  
Naveenkumar Anugu ◽  
Swathi Jalagam ◽  
Hari Prasad Kokatla

A transition-metal and hydride-free reductive aldol reaction has been developed for the synthesis of biologically active 3,3´-disubstituted oxindoles from isatin-derivatives using rongalite. In this protocol, rongalite plays the dual role...

2022 ◽  
Divyamahalakshmi Muthuraj ◽  
Raja Murugan ◽  
R Pavul Pavul Raj ◽  
Ganapathi Rao Kandregula ◽  
Kothandaraman Ramanujam

Developing an advanced separator that could stop the polysulfide shuttling remains a work-in-progress in the Li-S battery domain. Most of the work reported so far concentrates on functionalizing the commercial...

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