Tissue Preparation for Liquid Scintillation and Gamma Counting — the Counting Processes

2019 ◽  
pp. 45-86
Author(s):  
Howard J. Glenn ◽  
Lelio G. Colombetti
Keyword(s):  
Liquid Scintillation ◽  
Counting Processes ◽  
Tissue Preparation ◽  
Gamma Counting

Use of 125I-labeled-histamine-cyclosporin C for monitoring serum cyclosporine concentrations in transplantation patients.

1986 ◽  
Vol 32 (3) ◽  
pp. 492-495 ◽  
Author(s):  
P Y Wong ◽  
M Cheung ◽  
T K Yip ◽  
A V Mee
Keyword(s):  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Scintillation Counting ◽  
Gamma Counting ◽  
Analytical Recovery ◽  
Bile Extract ◽  
Good Agreement

Abstract We synthesized an 125I-labeled-histamine-cyclosporin C tracer, to obviate the use of tritiated tracer in radioimmunoassay of cyclosporine. With this tracer, the assay results varied linearly with concentration up to at least 800 micrograms/L. The within-assay CV was 6.6% at 39 micrograms/L, 4.2% at 100 micrograms/L, and 7.0% at 300 micrograms/L (n = 15). The between-assay CV was 10.0, 6.4, and 7.8% for the same respective concentrations. Comparison with an assay involving tritiated tracer (x) showed good agreement of results: y = 3.81 + 0.927x (r = 0.975, n = 604). Analytical recovery ranged from 100 to 106%. We also compared another commercially available radioiodinated tracer ("125Iodocyclosporin"; Immunonuclear Corp.). Our tracer appeared to be more specific for cyclosporine, as determined by assaying chromatographic fractions of bile extract from a patient being treated with cyclosporine. Results with use of our tracer compared favorably with those obtained with the tritiated tracer, and our assay has the advantages of gamma counting vs liquid-scintillation counting.

Comparison of Four Different Methods for the Recovery of Dotriacontane-16,17-14C from Small Animal Respiratory Tissue

1975 ◽  
Vol 14 (02) ◽  
pp. 185-191
Author(s):  
H. Haindl ◽  
N. Kmoch ◽  
G. Reznik ◽  
U. Mohr
Keyword(s):  
Freeze Drying ◽  
Liquid Scintillation ◽  
Small Animal ◽  
Respiratory Epithelium ◽  
Scintillation Counting ◽  
Tissue Preparation ◽  
Laboratory Equipment ◽  
Standard Solution ◽  
Tissue Maceration ◽  
Respiratory Tissue

SummaryThe efficiency and accuracy of four different methods of tissue preparation for the recovery of dotriacontane-16,17-14C (DOT-16,17-14C) from rat respiratory tissue were compared by means of liquid scintillation counting. A DOT-16, 17-14C standard solution was placed on the respiratory epithelium of the nasal apex, larynx, trachea and main bronchi. Combustion of the lyophilized organs in a Packard sample oxidizer revealed the highest recovery and accuracy (93—100%), sample oxidizing by combustion without lyophilization the lowest recovery and accuracy (73—92%). Tissue solubilization by the commercially available tissue solubilizer TS-1 revealed a better recovery and accuracy (89—95%) than tissue maceration by boiling in methanolic KOH and toluene extraction (74—91%). Depending on the laboratory equipment, lyophilization or tissue solubilization is preferred; maceration and toluene extraction as well as combustion without freeze-drying are disregarded for further investigations because of the cumbersome procedure as well as the low recovery and accuracy, respectively.

Evaluation of Alternative Counting Methods for Radioimmunoassay of Hepatitis-Associated Antigen (HB Ag)

1974 ◽  
Vol 20 (7) ◽  
pp. 733-737 ◽  
Author(s):  
William C Jordan ◽  
Vina Spiehler ◽  
Robert Haendiges ◽  
Edith Zak Helman
Keyword(s):  
Acetic Acid ◽  
Liquid Scintillation ◽  
Glacial Acetic Acid ◽  
Counting Efficiency ◽  
Antigen Test ◽  
High Background ◽  
Gamma Counting ◽  
Counting Methods ◽  
Direct Counting ◽  
Iodine 125

Abstract Alternative counting methods for the Abbott "Aus-RIA" hepatitis-associated antigen test have been devised and evaluated. These methods involve solubilization of the tube-bound radiolabeled complex with glacial acetic acid, transfer of the eluate to a counting vial of choice, and measurement by either a gamma scintillation or a liquid scintillation system. These methods, when compared to the direct counting of the AusRIA tube for sera from more than 250 patients and controls, gave a correlation coefficient of 0.94 to 0.99. Iodine-125 counting efficiencies averaged 65% both for direct counting of the AusRIA tube and for gamma counting of the eluate. By liquid scintillation counting, iodine-125 counting efficiency averaged 50%. This method avoids high background, chemiluminescence, and quench, and is easy to perform. By using glacial acetic acid to solubilize the labeled material, the eluate can be counted by various types of gamma counters as well as by liquid scintillation systems.

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

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