VALIDATION OF THE GEIGER-MULLER COUNTER MODEL OF BDMG-04-02 USING THE MONTE-CARLO TECHNIQUE

The model of the Geiger-Muller counter as the internal part of BDMG-04-02 detection unit in the calibration fa-cility UPGD-2 was developedin MCNP6.2. The different methods are used for the determination of the Geiger-Muller counter response. The F1 and F8 tally applicability is briefly described. BDMG-04-02 model was validated by comparative analysis of the calculated results and experimental values of the counter responses that obtained on the UPGD-2 calibration facility. Additionally, the absolute, geometric and intrinsic registration efficiency of BDMG-04-02 was determined. The paper has been emphasized the disadvantages of using the method of direct counting of the electrons on the surface of the Geiger-Muller counter (F1).

Penentuan Komposisi Bahan Bakar Nabati Dalam Bahan Bakar Minyak Campuran Menggunakan Metode Direct Counting C-14

ABSTRAK. Telah dilakukan penentuan komposisi bahan bakar nabati (BBN) dalam bahan bakar minyak campuran (BBMC) dengan metode direct counting C-14. Penentuan komposisi BBN dalam BBMC dilakukan dengan cara memipet 10 mL BBMC ke dalam vial gelas kemudian ditambahkan 10 mL larutan sintilator Ultima Gold F (UGF) ke dalamnya. Vial tersebut dikocok agar campuran menjadi homogen kemudian dicacah menggunakan LSC (Liquid Scintillation Counter) Elmer Perkin 2900TR selama 20 menit sebanyak 30 siklus. Hasil pencacahan ditampilkan dalam bentuk tSIE (transformed external standard spectrum) dan cpm (cacahan permenit). Hasil analisis memperlihatkan nilai cpm yang meningkat seiring kenaikan persentase BBN dalam BBMC. Nilai cpm terendah dan tertinggi untuk sampel bensin, avtur, dan solar berturut-turut adalah 14,2363 dan 62,0343, 10,664 dan 44,535, serta 9,410 dan 61,789. Terdapat korelasi kuat antara nilai tSIE dan nilai cpm pada bensin dan solar tapi tidak pada avtur. Hasil analisis terhadap sampel uji menunjukkan bahwa sampel tersebut berada di luar grafik deret sampel. Metode direct counting ini diharapkan dapat menjadi referensi dalam uji mutu BBMC.ABSTRACT. The composition of biofuel (BF) in mixed oil fuel (MOF) has been determined using the C-14 direct counting method. Determination of the composition of BF in MOF was carried out by pipetting 10 mL of BBMC into a glass vial and then adding 10 mL of Ultima Gold F (UGF) scintillator solution into it. The vial was shaken so that the mixture became homogeneous and then counted using the Elmer Perkin 2900TR LSC (Liquid Scintillation Counter) for 20 minutes for 30 cycles. The results of the counting are displayed in the form of tSIE (transformed external standard spectrum) and cpm (counts per minute). The results of the analysis show that the value of cpm increases with the increase in the percentage of BF in MOF. The lowest and highest cpm values for gasoline, avtur, and diesel samples were 14.2363 and 62.0343, 10.664 and 44.535, and 9.410 and 61.789, respectively. There is a strong correlation between tSIE and cpm values for gasoline and diesel but not for avtur. The results of the analysis of the test sample indicate that the sample is outside the sample series graph. This direct counting method is expected to be a reference in the BBMC quality test.

Determination of Kinetic Properties in Unimolecular Dissociation of Complex Systems from Graph-Theory Based Analysis of an Ensemble of Reactive Trajectories.

<div>We describe and apply a general approach based on graph-theory to obtain kinetic and structural properties from direct dynamics simulations. In particular, we focus on the unimolecular fragmentation of complex systems in which, prior to dissociation, different events can take place, and notably isomerizations and formation of ion-molecule complex.</div><div>3-state and 4-state kinetic models are thus obtained and rate constants for global or specific pathways are obtained from direct counting and flux calculation, both being in agreement.<br />Finally, we show how a theoretical mass spectrum can also be obtained automatically.<br /></div>

Analysis of NGS-Ministr as A New Detection Strategy Chromosomal Trisomy 16 of Fetal Materials in Miscarriages

Background: Although many technologies can identify chromosomal abnormalities, they can’t completely replace the advantages of short tandem repeat (STR) genotyping technology. The miniSTR typing based on next generation sequencing (NGS-miniSTR) with high throughput, accuracy and sensitivity has the potential to determine the trisomy and overcome the limitations of conventional STR techniques, which never be tried. The study aimed to explore the value of the NGS-miniSTR in trisomy testing, which is an extension and improvement of the methodology of chromosomal detection technology.Methods: A total of 140 fetal materials in miscarriages (70 trisomy 16 and 70 normal karyotypes) screened by copy-number variation sequencing (CNV-seq) were genotyped using the customed panel containing 11 miniSTRs based on NGS. Direct counting method, chi-square (χ2) test, k-means clustering analysis and Mahalanobis distance were operated to compare the difference of miniSTR between trisomy 16 and normal group, and then analyze whether trisomy 16 could be identified.Results: All miniSTR results based on NGS were successfully obtained, except for D16S771 in 21 samples. There were significant differences in the average depth of coverage at each locus in each sample. Direct counting method and chi-square (χ2) test showed significant differences in allelic pattern and read ratio between trisomy and normal group, respectively. Almost all samples correctly divided into 2 clusters based on diallelic STR reads ratios according to k-means clustering analysis. In addition, the Mahalanobis distance showed that D16S771 had multiple outliers. Conclusion: A new strategy of miniSTR-NGS was firstly introduced to successfully detect all samples of trisomy 16 in the fetal material in miscarriages, which revealed that the allelic pattern and allelic read ratio could be effective indexes to identify the number of chromosomes.

Determination of Kinetic Properties in Unimolecular Dissociation of Complex Systems from Graph-Theory Based Analysis of an Ensemble of Reactive Trajectories.

<div>We describe and apply a general approach based on graph-theory to obtain kinetic and structural properties from direct dynamics simulations. In particular, we focus on the unimolecular fragmentation of complex systems in which, prior to dissociation, different events can take place, and notably isomerizations and formation of ion-molecule complex.</div><div>3-state and 4-state kinetic models are thus obtained and rate constants for global or specific pathways are obtained from direct counting and flux calculation, both being in agreement.<br>Finally, we show how a theoretical mass spectrum can also be obtained automatically.<br></div>

Determination of Kinetic Properties in Unimolecular Dissociation of Complex Systems from Graph-Theory Based Analysis of an Ensemble of Reactive Trajectories.

<div>We describe and apply a general approach based on graph-theory to obtain kinetic and structural properties from direct dynamics simulations. In particular, we focus on the unimolecular fragmentation of complex systems in which, prior to dissociation, different events can take place, and notably isomerizations and formation of ion-molecule complex.</div><div>3-state and 4-state kinetic models are thus obtained and rate constants for global or specific pathways are obtained from direct counting and flux calculation, both being in agreement.<br>Finally, we show how a theoretical mass spectrum can also be obtained automatically.<br></div>

Closed frequent itemsets mining based on It-Tree

In the last decade, the amount of collected data, in various computer science applications, has grown considerably. These large volumes of data need to be analysed in order to extract useful hidden knowledge. This work focuses on association rule extraction. This technique is one of the most popular in data mining. Nevertheless, the number of extracted association rules is often very high, and many of them are redundant. In this paper, we propose an algorithm, for mining closed itemsets, with the construction of an it-tree. This algorithm is compared with the DCI (direct counting & intersect) algorithm based on min support and computing time. CHARM is not memery-efficient. It needs to store all closed itemsets in the memory. The lower min-sup is, the more frequent closed itemsets there are so that the amounts of memory used by CHARM are increasing.

A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Multi-subunit ring-ATPases carry out a myriad of biological functions, including genome packaging in viruses. Though the basic structures and functions of these motors have been well-established, the mechanisms of ATPase firing and motor coordination are poorly understood. Here, by direct counting using single-molecule fluorescence, we have determined that the active bacteriophage T4 DNA packaging motor consists of five subunits of gp17. By systematically doping motors with an ATPase-defective subunit and selecting single motors containing a precise count of active/inactive subunit(s), we found, unexpectedly, that the packaging motor can tolerate an inactive sub-unit. However, motors containing an inactive subunit(s) exhibit fewer DNA engagements, a higher failure rate in encapsidation, reduced packaging velocity, and increased pausing. These findings suggest a new packaging model in which the motor, by re-adjusting its grip on DNA, can skip an inactive subunit and resume DNA translocation, contrary to the prevailing notion of strict coordination amongst motor subunits of other packaging motors.

Public Spaces and Concentrations of Child Labourers in Ibadan Municipality, Nigeria

Against the background of the solution-defying incidence of child labourers in Nigerian cities, this study examined the relationship between public spaces and incidence of child labourers in Ibadan Municipality, Nigeria. Data were obtained through direct counting of child labourers and cursory observation of the physical and environmental compositions of their places of operation. The direct counting was conducted for seven days of the week in the morning, afternoon and evening within defined activity-nuclei purposively selected across three densities of residential areas of the municipality: high, medium and low. The data collected were subjected to descriptive statistics. Findings showed that the incidence and categories of child labourers varied with public spaces and density of residential areas, and also followed the concentric, sector and multiple nuclei theories of urban land use. The study, therefore, recommended that urban planners and government have roles to play in offering lasting solution to the menace of child labour.