Inhibitors of DNA Synthesis in Senescent and Quiescent Human Diploid Fibroblasts

2020 ◽  
pp. 137-147
Author(s):  
Gretchen H. Stein
Keyword(s):  
Dna Synthesis ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts

Complement-dependent induction of DNA synthesis and proliferation of human diploid fibroblasts

1980 ◽  
Vol 105 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Terry I. Korotzer ◽  
James A. Clagett ◽  
William P. Kolb ◽  
Roy C. Page
Keyword(s):  
Dna Synthesis ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts

scholarly journals Cell population heterogeneity in the inducibility of DNA synthesis in human diploid fibroblasts.

1981 ◽  
Vol 89 (2) ◽  
pp. 194-197 ◽  
Author(s):  
M V Rao
Keyword(s):  
Dna Synthesis ◽  
Cytochalasin B ◽  
Nuclear Dna ◽  
S Phase ◽  
Population Heterogeneity ◽  
Diploid Fibroblasts ◽  
Binucleate Cells ◽  
Human Diploid Fibroblasts ◽  
Significant Difference ◽  
Sister Cells

The initiation of nuclear DNA synthesis has been studied in cytochalasin B (CB)-induced binucleate human diploid fibroblasts (WI-38 cells). Mitotic cells from different passage levels were rendered binucleate by a brief pulse of CB. The cells were then washed free of the drug, and DNA synthesis was studied by [3H]thymidine labeling. The results showed that, in a small percentage of binucleate cells, one nucleus was labeled (S phase) and the other nucleus was unlabeled (G1 phase). There was no significant difference in the percentage of these cells with increasing passage levels. The results of this study suggest that some WI-38 cells retire from the cell cycle at different passage levels, and thereby become refractory to inducers of nuclear DNA synthesis generated by sister cells in S phase.

scholarly journals Template activity of chromatin during stimulation of cellular proliferation in human diploid fibroblasts

1971 ◽  
Vol 122 (2) ◽  
pp. 189-195 ◽  
Author(s):  
John Farber ◽  
Giovanni Rovera ◽  
Renato Baserga
Keyword(s):  
Dna Synthesis ◽  
Rna Synthesis ◽  
Cellular Proliferation ◽  
Calf Serum ◽  
Foetal Calf Serum ◽  
Template Activity ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts ◽  
Chromatin Template ◽  
Stimulation Of

1. Contact-inhibited confluent monolayers of WI-38 human diploid fibroblasts can be stimulated to divide by replacing the medium with fresh medium containing 30′ foetal calf serum. 2. Of the cells 40–75′ are stimulated to divide with a peak DNA synthesis between 15 and 21h and a peak mitotic index between 28 and 30h after stimulation. 3. In the first 12h before the initiation of DNA synthesis there is a biphasic increase in the incorporation of [3H]uridine into RNA of whole cells. 4. This is paralleled by a similar biphasic stimulation of chromatin template activity measured in vitro in a system in which purified cell chromatin is incubated with an exogenous RNA polymerase isolated from Escherichia coli. 5. The changes in chromatin template activity are believed to represent activation of the genome, with more sites available for RNA synthesis, and to account almost entirely for the changes in RNA synthesis occurring in the whole cell.

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