Effects of Synthetic Analogs and Fragments of Bovine Parathyroid Hormone on Adenosine 3′,5′-Monophosphate Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis in Rabbit Costal Chondrocytes in Culture: Structure-Activity Relations

Endocrinology ◽  
1985 ◽  
Vol 116 (6) ◽  
pp. 2536-2542 ◽  
Author(s):  
TERUKO TAKANO ◽  
MASAHARU TAKIGAWA ◽  
EIJI SHIRAI ◽  
FUJIO SUZUKI ◽  
MICHAEL ROSENBLATT
Keyword(s):  
Parathyroid Hormone ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Glycosaminoglycan Synthesis ◽  
Synthetic Analogs ◽  
Ornithine Decarboxylase Activity ◽  
Structure Activity ◽  
Structure Activity Relations ◽  
Bovine Parathyroid Hormone

The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture

1987 ◽  
Vol 66 (1) ◽  
pp. 84-87 ◽  
Author(s):  
T. Takano ◽  
M. Takigawa ◽  
E. Shirai ◽  
K. Nakagawa ◽  
M. Sakuda ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Ornithine Decarboxylase ◽  
Septal Cartilage ◽  
Decarboxylase Activity ◽  
Condylar Cartilage ◽  
Polyamine Biosynthesis ◽  
Glycosaminoglycan Synthesis ◽  
Mandibular Condylar Cartilage ◽  
Nasal Septal Cartilage

Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.

Effects of parathyroid hormone on ornithine decarboxylase activity in human osteosarcoma cells

1991 ◽  
Vol 286 (2) ◽  
pp. 316-322 ◽  
Author(s):  
Hitoshi Goto ◽  
Isao Matsui-Yuasa ◽  
Shuzo Otani ◽  
Seiji Morisawa ◽  
Yoshiki Nishizawa ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Ornithine Decarboxylase Activity ◽  
Human Osteosarcoma Cells

Induction of ornithine decarboxylase activity in isolated chicken osteoblasts by parathyroid hormone: The role of cAMP and calcium

1988 ◽  
Vol 43 (1) ◽  
pp. 7-18 ◽  
Author(s):  
C. W. G. M. Löwik ◽  
A. A. Olthof ◽  
J. P. T. M. van Leeuwen ◽  
J. K. van Zeeland ◽  
M. P. M. Herrmann-Erlee
Keyword(s):  
Parathyroid Hormone ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity

Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

1978 ◽  
Vol 39 (02) ◽  
pp. 496-503 ◽  
Author(s):  
P A D’Amore ◽  
H B Hechtman ◽  
D Shepro
Keyword(s):  
Endothelial Cells ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Aortic Endothelial Cells ◽  
Ornithine Decarboxylase Activity ◽  
Rate Limiting Step ◽  
Bovine Aortic Endothelial Cells ◽  
Limiting Step ◽  
Rate Limiting ◽  
Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

Sign in / Sign up

Export Citation Format

Share Document