Estrogen Inhibits Luteinizing Hormone (LH), but Not Follicle-Stimulating Hormone Secretion in Hypophysectomized Pituitary-Grafted Rats Receiving Pulsatile LH-Releasing Hormone Infusions*

Endocrinology ◽  
1988 ◽  
Vol 123 (1) ◽  
pp. 622-630 ◽  
Author(s):  
FRANK J. STROBL ◽  
JON E. LEVINE
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Hormone Secretion ◽  
Releasing Hormone ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

DEVELOPMENT AND APPLICATION OF HOMOLOGOUS RADIOIMMUNOASSAYS FOR PORCINE GONADOTROPHINS

1979 ◽  
Vol 81 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J. L. VANDALEM ◽  
CH. BODART ◽  
G. PIRENS ◽  
J. CLOSSET ◽  
G. HENNEN
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
Releasing Hormone ◽  
Porcine Serum ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

Antisera were raised against highly purified preparations of porcine luteinizing hormone (pLH) and follicle-stimulating hormone (pFSH). Highly specific and sensitive radioimmunoassay systems were developed. The antisera to LH and FSH were used at working dilutions of 1: 500 000 and 1: 200 000 respectively and the sensitivities of the assays were 0·1 ng LH/ml serum (3 × 10−12 mol/l) and 0·5 ng FSH/ml serum (1·5 × 10−11 mol/l). The LH and FSH preparations used as standards were 1·2 and 81 times as potent as NIH-LH-S15 and NIH-FSH-P1 respectively. Both assays were validated and adapted for the measurement of the gonadotrophin content of porcine serum. The concentrations of LH and FSH in blood were measured simultaneously in prepubertal sows throughout a 24 h period, in adult sows during the oestrous cycle and in both prepubertal and adult animals after treatment with LH releasing hormone.

Sign in / Sign up

Export Citation Format

Share Document