ovariectomized rats
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Author(s):  
Piyachat Chansela ◽  
Bubphachat Potip ◽  
Jittima Weerachayaphorn ◽  
Niwat Kangwanrangsan ◽  
Natsasi Chukijrungroat ◽  
...  

2022 ◽  
Vol 8 ◽  
Author(s):  
Yujie Zhu ◽  
Shucheng Liu ◽  
Fengfeng Mei ◽  
Meihui Zhao ◽  
Guanghua Xia ◽  
...  

Osteoporosis is a global health problem, and it is of great significance to replace the drugs with natural functional factors. In this study, we investigated the antiosteoporotic activity of lipids prepared from Tilapia nilotica fish head lipids (THLs) in the ovariectomized osteoporosis rats. THLs are composed of neutral lipids (NL, 77.84%), phospholipids (PL, 11.86%), and glycolipids (GL, 6.47%). There were apparent differences in the fatty acid composition of disparate components, and PL contains the most abundant Ω-3 polyunsaturated fatty acids. The results proved that THLs could improve bone microstructure, increase bone mineral density, and decrease bone resorption. To illustrate the antiosteoporotic mechanism, we analyzed the changes in gut microbial communities, proinflammation factors, serum metabolites, and metabolic pathways. Further study on gut microbiota showed that THLs significantly decreased the content of Alistipes in the gut and dramatically increased the beneficial bacteria such as Oscillospira, Roseburia, and Dubosiella. Meanwhile, proinflammation factors of serum in OVX rats decreased significantly, and metabolites were changed. Therefore, we speculated that THLs improved bone loss through reducing inflammation and changing the metabolites and metabolic pathways such as arachidonic acid metabolism and primary bile acid metabolism, etc., by altering gut microbiota. The results indicated that THLs could be a functional factor with antiosteoporotic activity.


2022 ◽  
Vol 6 (1) ◽  
pp. 01-05
Author(s):  
Armine V. Grigoryan ◽  
Alexander B. Blazhev ◽  
Tatyana M. Betova ◽  
Aneliya A. Dimitrova

Estradiol is an estrogen steroid hormone and is produced basically within the follicles of the ovaries. The decrease in serum estrogens concentration at menopause disrupts the metabolic balance, changes the lipid profile leading to visceral obesity, which caused an increase in serum estradiol levels, through aromatase activity. Estrogen deficiency also is a reason for the development of osteoporosis.We investigated the serum estradiol levels and changes in bone alpha estrogen receptor expression in ovariectomized rats. For this purpose, we used 20 female Wistar rats at reproductive age - 2 months divided into 2 groups: group 1 (G1)-10 animals were ovariectomized and group 2 (G2)-10 of which were sham-operated. All animals of G1 showed weight gain compared to group G2. The results showed that the values of serum 17β-estradiol in rats of G1 statistically increased compared to G2 (p <0.05). Immunohistochemical analysis revealed no difference in estrogen receptor expression between the both groups. Histomorphological analysis of femur from G1 showed the presence of pronounced osteoporosis. Ovariectomy led to the development of obesity, which caused an increase in serum estradiol levels, through aromatase activity, but this process did not prevent bone tissue from developing osteoporosis.


Materials ◽  
2022 ◽  
Vol 15 (1) ◽  
pp. 333
Author(s):  
Anna Skic ◽  
Iwona Puzio ◽  
Grzegorz Tymicki ◽  
Paweł Kołodziej ◽  
Marta Pawłowska-Olszewska ◽  
...  

The investigations on the response of bone tissue under different loading conditions are important from clinical and engineering points of view. In this paper, the influence of nesfatin-1 administration on rat humerus mechanical properties was analyzed. The classical three-point bending and impact tests were carried out for three rat bone groups: control (SHO), the humerus of animals under the conditions of established osteopenia (OVX), and bones of rats receiving nesfatin-1 after ovariectomy (NES). The experiments proved that the bone strength parameters measured under various mechanical loading conditions increased after the nesfatin-1 administration. The OVX bones were most susceptible to deformation and had the smallest fracture toughness. The SEM images of humerus fracture surface in this group showed that ovariectomized rats had a much looser bone structure compared to the SHO and NES females. Loosening of the bone structure was also confirmed by the densitometric and qualitative EDS analysis, showing a decrease in the OVX bones’ mineral content. The samples of the NES group were characterized by the largest values of maximum force obtained under both quasi-static and impact conditions. The energies absorbed during the impact and the critical energy for fracture (from the three-point bending test) were similar for the SHO and NES groups. Statistically significant differences were observed between the mean Fi max values of all analyzed sample groups. The obtained results suggest that the impact test was more sensitive than the classical quasi-static three-point bending one. Hence, Fi max could be used as a parameter to predict bone fracture toughness.


2021 ◽  
Vol 20 (4) ◽  
pp. 459-464
Author(s):  
Joanna Suliburska ◽  
◽  
Iskandar Azmy Harahap ◽  
Natalia Wawrzyniak ◽  
Anna Gramza-Michałowska ◽  
...  

2021 ◽  
Vol 20 (4) ◽  
pp. 459-464
Author(s):  
Joanna Suliburska ◽  
◽  
Iskandar Azmy Harahap ◽  
Natalia Wawrzyniak ◽  
Anna Gramza-Michałowska ◽  
...  

Author(s):  
Weiwei He ◽  
Louise M. A. Jakobsen ◽  
Line F. Zachariassen ◽  
Axel K. Hansen ◽  
Henrik J. Andersen ◽  
...  

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