Determination of Plasma Corticoids by Competitive Protein-Binding Analysis Using Gel Filtration

1964 ◽  
Vol 24 (9) ◽  
pp. 919-923 ◽  
Author(s):  
BEVERLEY E. PEARSON MURPHY ◽  
CHAUNCEY J. PATTEE ◽  
Elizabeth Inglis ◽  
Wan-Ching Sun
Keyword(s):  
Protein Binding ◽  
Gel Filtration ◽  
Binding Analysis ◽  
Competitive Protein Binding

FURTHER ASSESSMENT OF THE RELIABILITY OF PROGESTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S171-S187 ◽  
Author(s):  
M. L. A. de Souza ◽  
H. O. Williamson ◽  
L. O. Moody ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Purification Procedure ◽  
Displacement Effect ◽  
Plasma Progesterone ◽  
Binding Analysis ◽  
Extraction And Purification ◽  
Competitive Protein Binding ◽  
Proper Consideration

ABSTRACT Experimental variables influencing the determination of plasma progesterone by competitive protein binding analysis have been further assessed. The precision of the method has been improved by additional mechanisation. Method interfering factors introduced by the extraction and purification procedure have been evaluated quantitatively. Another type of interfering material is present in plasma extracts from which all progesterone has been removed. The displacement effect of this material is additive to that derived from solvent and chromagram residues. Unless the various types of interfering material are taken into proper consideration, competitive protein binding methods for steroids may yield erroneous results.

EVALUATION OF A SIMPLIFIED METHOD FOR DETERMINING SERUM THYROXINE BY COMPETITIVE PROTEIN BINDING ANALYSIS

1970 ◽  
Vol 64 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Stephen C. Thorson ◽  
Ronald Tsujikawa ◽  
James L. Brown ◽  
Robert T. Morrison ◽  
Hamish W. McIntosh
Keyword(s):  
Protein Binding ◽  
Clinical Application ◽  
Thyroid Status ◽  
Serum Thyroxine ◽  
Binding Analysis ◽  
Simplified Method ◽  
Competitive Protein Binding ◽  
Hypothyroid Patients

ABSTRACT Serum thyroxine concentrations were determined in 66 euthyroid, 30 hyperthyroid and 13 hypothyroid patients using both the established Murphy method and a simplified method of competitive protein binding analysis. A diagnosis compatibility of 96% was found with both methods indicating that the simplified method has comparable clinical application as an initial screen of thyroid status.

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