Determination of the binding of thyroxine to plasma proteins by competitive protein-binding analysis

1973 ◽  
Vol 45 (1) ◽  
pp. 37-46 ◽  
Author(s):  
André Elewaut
Keyword(s):  
Protein Binding ◽  
Plasma Proteins ◽  
Binding Analysis ◽  
Competitive Protein Binding

scholarly journals Plasma Cortisone in Man: Its Determination, Physiological Variation, and Significance

1980 ◽  
Vol 17 (5) ◽  
pp. 227-232 ◽  
Author(s):  
J D Few ◽  
G C Cashmore
Keyword(s):  
Protein Binding ◽  
Simultaneous Determination ◽  
Cortisol Level ◽  
Paper Chromatography ◽  
Plasma Proteins ◽  
Hepatic Uptake ◽  
Physiological Variation ◽  
Competitive Protein Binding ◽  
Response To Exercise

In a method for the simultaneous determination of cortisone, cortisol, and corticosterone, the extracted steroids are separated using paper chromatography and assayed by competitive protein binding. Evidence of the reliability of the method, particularly with respect to the determination of cortisone, is presented. Only trivial changes in cortisone level were detected in response to exercise, surgery, and ACTH, even though marked changes in cortisol level were simultaneously recorded. It is suggested that the inertia in plasma cortisone level may be related to its lesser degree of binding to plasma proteins, relative to cortisol, which would permit more rapid hepatic uptake and possible accumulation of a large extravascular pool of cortisone.

Determination of Plasma Corticoids by Competitive Protein-Binding Analysis Using Gel Filtration

1964 ◽  
Vol 24 (9) ◽  
pp. 919-923 ◽  
Author(s):  
BEVERLEY E. PEARSON MURPHY ◽  
CHAUNCEY J. PATTEE ◽  
Elizabeth Inglis ◽  
Wan-Ching Sun
Keyword(s):  
Protein Binding ◽  
Gel Filtration ◽  
Binding Analysis ◽  
Competitive Protein Binding

FURTHER ASSESSMENT OF THE RELIABILITY OF PROGESTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S171-S187 ◽  
Author(s):  
M. L. A. de Souza ◽  
H. O. Williamson ◽  
L. O. Moody ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Purification Procedure ◽  
Displacement Effect ◽  
Plasma Progesterone ◽  
Binding Analysis ◽  
Extraction And Purification ◽  
Competitive Protein Binding ◽  
Proper Consideration

ABSTRACT Experimental variables influencing the determination of plasma progesterone by competitive protein binding analysis have been further assessed. The precision of the method has been improved by additional mechanisation. Method interfering factors introduced by the extraction and purification procedure have been evaluated quantitatively. Another type of interfering material is present in plasma extracts from which all progesterone has been removed. The displacement effect of this material is additive to that derived from solvent and chromagram residues. Unless the various types of interfering material are taken into proper consideration, competitive protein binding methods for steroids may yield erroneous results.

EVALUATION OF A SIMPLIFIED METHOD FOR DETERMINING SERUM THYROXINE BY COMPETITIVE PROTEIN BINDING ANALYSIS

1970 ◽  
Vol 64 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Stephen C. Thorson ◽  
Ronald Tsujikawa ◽  
James L. Brown ◽  
Robert T. Morrison ◽  
Hamish W. McIntosh
Keyword(s):  
Protein Binding ◽  
Clinical Application ◽  
Thyroid Status ◽  
Serum Thyroxine ◽  
Binding Analysis ◽  
Simplified Method ◽  
Competitive Protein Binding ◽  
Hypothyroid Patients

ABSTRACT Serum thyroxine concentrations were determined in 66 euthyroid, 30 hyperthyroid and 13 hypothyroid patients using both the established Murphy method and a simplified method of competitive protein binding analysis. A diagnosis compatibility of 96% was found with both methods indicating that the simplified method has comparable clinical application as an initial screen of thyroid status.

Sign in / Sign up

Export Citation Format

Share Document