scholarly journals Substantial Production of Ghrelin by a Human Medullary Thyroid Carcinoma Cell Line

2001 ◽  
Vol 86 (10) ◽  
pp. 4984-4990 ◽  
Author(s):  
Naotetsu Kanamoto ◽  
Takashi Akamizu ◽  
Hiroshi Hosoda ◽  
Yuji Hataya ◽  
Hiroyuki Ariyasu ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Medullary Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Thyroid Carcinoma Cell

Biosynthesis of an asparagine-linked oligosaccharide-containing calcitonin by a rat medullary thyroid carcinoma cell line

Biochemistry ◽  
1986 ◽  
Vol 25 (10) ◽  
pp. 2995-3000 ◽  
Author(s):  
Kathleen Corey Flanders ◽  
Roger S. Birnbaum ◽  
Walter C. Mahoney ◽  
Bernard A. Roos
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Medullary Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Thyroid Carcinoma Cell

Point Mutation of the RetProto-oncogene in the TT Human Medullary Thyroid Carcinoma Cell Line

1995 ◽  
Vol 207 (3) ◽  
pp. 1022-1028 ◽  
Author(s):  
F. Carlomagno ◽  
D. Salvatore ◽  
M. Santoro ◽  
V. Defranciscis ◽  
L. Quadro ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Point Mutation ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Medullary Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Thyroid Carcinoma Cell

scholarly journals Expression and processing of procholecystokinin in a rat medullary thyroid carcinoma cell line

1990 ◽  
Vol 271 (1) ◽  
pp. 31-36 ◽  
Author(s):  
L Ødum ◽  
J F Rehfeld
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Gel Chromatography ◽  
Medullary Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Before And After ◽  
Thyroid Carcinoma Cell

A rat medullary thyroid carcinoma cell line, CA-77, was shown to express the cholecystokinin (CCK) gene. Measurements using a library of sequence-specific radioimmunoassays before and after enzymic treatment of extracts and chromatographic fractions showed that the cells contained 1.0 pmol of alpha-carboxyamidated cholecystokinins/10(6) cells, 0.4 pmol of glycine-extended intermediates/10(6) cells and 1.0 pmol of further C-terminal-extended pro-CCK/10(6) cells. Gel chromatography and reverse-phase h.p.l.c. revealed both sulphated and nonsulphated CCK-8 in the cells. The growth medium contained in addition alpha-amidated CCK-33, glycine-extended CCK-8 and pro-CCK. Exposure to 0.1 microM-dexamethasone for 6 days increased the cellular content and secretion of all of the described CCK peptides by 2-3-fold. The increase was first noted after 3 days of treatment. Monensin inhibited the synthesis of alpha-carboxyamidated CCK and the secretion of all of the CCK forms measured. Colchicine at a low concentration (0.2 mumol/l) apparently increased the synthesis and secretion of alpha-carboxyamidated CCK, whereas higher concentrations inhibited CCK synthesis. Finally, chloroquine inhibited the alpha-carboxyamidation of CCK. We conclude that the CA-77 cell line is a useful tool for studies of the expression and post-translational processing of pro-CCK.

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