Human embryonic stem cells are characterized by distinct patterns of histone modifications in comparison with cells of feeder layer

2008 ◽  
Vol 2008 (Spring) ◽  
Author(s):  
Eva Bartova ◽  
Abdrea Harnicarova ◽  
Jana Krejci ◽  
Gabriela Galiova ◽  
Stanislav Kozubek
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Histone Modifications ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Layer

scholarly journals Rewiring of transcription factor binding in differentiating human embryonic stem cells is constrained by DNA sequence repeat symmetry

2018 ◽  
Author(s):  
Matan Goldshtein ◽  
David B. Lukatsky
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Embryonic Stem Cells ◽  
Dna Binding ◽  
Dna Sequence ◽  
Histone Modifications ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Sequence Repeat ◽  
Dna Repeat

ABSTRACTWe analyze design principles of transcription factor (TF) recognition by genomic DNA in differentiating human embryonic stem cells for 36 TFs and five histone modifications in four developmental layers, using the data recently measured by Tsankov et al. This analysis reveals that DNA sequence repeat symmetry plays a central role in defining TF-DNA binding preferences across different developmental layers. In particular, we find that different TFs bind similar symmetry patterns within a given developmental layer. While the TF cluster content undergoes modifications upon transitions between different developmental layers, most TFs possess dominant preferences for similar DNA repeat symmetry types. Histone modifications also exhibit strong preferences for similar DNA repeat symmetry patterns, with the symmetry strength differentiating between different histone modifications. Overall, our findings show that despite the enormous sequence complexity of the TF-DNA binding landscape in differentiating human embryonic stem cells, this landscape can be quantitatively characterized in simple terms, using the notion of DNA sequence repeat symmetry.

scholarly journals Human Menstrual Blood-Derived Mesenchymal Cells as New Human Feeder Layer System for Human Embryonic Stem Cells

Cell Medicine ◽  
2014 ◽  
Vol 7 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Danúbia Silva Dos Santos ◽  
Vanessa Carvalho Coelho De Oliveira ◽  
Karina Dutra Asensi ◽  
Leandro Vairo ◽  
Adriana Bastos Carvalho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mesenchymal Cells ◽  
Menstrual Blood ◽  
Feeder Layer ◽  
Layer System

scholarly journals Available human feeder cells for the maintenance of human embryonic stem cells

Reproduction ◽  
2004 ◽  
Vol 128 (6) ◽  
pp. 727-735 ◽  
Author(s):  
Jung Bok Lee ◽  
Ji Min Song ◽  
Jeoung Eun Lee ◽  
Jong Hyuk Park ◽  
Sun Jong Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Layer ◽  
Feeder Cells ◽  
Embryonic Fibroblasts ◽  
Differentiation Capacity ◽  
Human Adult ◽  
Feeder Layers

Mouse embryonic fibroblasts (MEFs) have been previously used as feeder cells to support the growth of human embryonic stem cells (hESCs). In this study, human adult uterine endometrial cells (hUECs), human adult breast parenchymal cells (hBPCs) and embryonic fibroblasts (hEFs) were tested as feeder cells for supporting the growth of hESCs to prevent the possibility of contamination from animal feeder cells. Cultured hUECs, hBPCs and hEFs were mitotically inactivated and then plated. hESCs (Miz-hES1, NIH registered) initially established on mouse feeder layers were transferred onto each human feeder layer and split every 5 days. The morphology, expression of specific markers and differentiation capacity of hESCs adapted on each human feeder layer were examined. On hUEC, hBPC and hEF feeder layers, hESCs proliferated for more than 90, 50 and 80 passages respectively. Human feeder-based hESCs were positive for stage-specific embryonic antigen (SSEA)-3 and -4, and Apase; they also showed similar differentiation capacity to MEF-based hESCs, as assessed by the formation of teratomas and expression of tissue-specific markers. However, hESCs cultured on hUEC and hEF feeders were slightly thinner and flatter than MEF- or hBPC-based hESCs. Our results suggest that, like MEF feeder layers, human feeder layers can support the proliferation of hESCs without differentiation. Human feeder cells have the advantage of supporting more passages than when MEFs are used as feeder cells, because hESCs can be uniformly maintained in the undifferentiated stage until they pass through senescence. hESCs established and/or maintained under stable xeno-free culture conditions will be helpful to cell-based therapy.

scholarly journals Generation of definitive endoderm from human embryonic stem cells cultured in feeder layer-free conditions

2008 ◽  
Vol 3 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Charles C King ◽  
Gillian M Beattie ◽  
Ana D Lopez ◽  
Alberto Hayek
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Definitive Endoderm ◽  
Feeder Layer ◽  
Cells Cultured

Cheap and Simple: Human Tube Mesenchymal Stem Cells as Feeder Layer for Human Embryonic Stem Cells

2016 ◽  
Vol 1 (1) ◽  
pp. 28-41
Author(s):  
Pelatti M ◽  
◽  
Secco M ◽  
Coatti G ◽  
Gomes J ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Layer
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