scholarly journals Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo

Development ◽  
2014 ◽  
Vol 141 (7) ◽  
pp. 1599-1599 ◽  
Author(s):  
B. C. Cox ◽  
R. Chai ◽  
A. Lenoir ◽  
Z. Liu ◽  
L. Zhang ◽  
...  
Keyword(s):  
Hair Cell ◽  
Neonatal Mouse ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

scholarly journals Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo

Development ◽  
2014 ◽  
Vol 141 (4) ◽  
pp. 816-829 ◽  
Author(s):  
B. C. Cox ◽  
R. Chai ◽  
A. Lenoir ◽  
Z. Liu ◽  
L. Zhang ◽  
...  
Keyword(s):  
Hair Cell ◽  
Neonatal Mouse ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

scholarly journals Multiple supporting cell subtypes are capable of spontaneous hair cell regeneration in the neonatal mouse cochlea

Development ◽  
2019 ◽  
Vol 146 (4) ◽  
pp. dev171009 ◽  
Author(s):  
Melissa M. McGovern ◽  
Michelle R. Randle ◽  
Candice L. Cuppini ◽  
Kaley A. Graves ◽  
Brandon C. Cox
Keyword(s):  
Hair Cell ◽  
Supporting Cell ◽  
Neonatal Mouse ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

scholarly journals Simultaneous gentamicin-mediated damage and Atoh1 overexpression promotes hair cell regeneration in the neonatal mouse utricle

2021 ◽  
Vol 398 (1) ◽  
pp. 112395
Author(s):  
Xiaoqing Qian ◽  
Rui Ma ◽  
Xinwei Wang ◽  
Xinda Xu ◽  
Juanmei Yang ◽  
...  
Keyword(s):  
Hair Cell ◽  
Neonatal Mouse ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

scholarly journals TUB and ZNF532 Promote the Atoh1-Mediated Hair Cell Regeneration in Mouse Cochleae

2021 ◽  
Vol 15 ◽  
Author(s):  
Zhenhang Xu ◽  
Vikrant Rai ◽  
Jian Zuo
Keyword(s):  
Transcription Factors ◽  
Hair Cell ◽  
Ex Vivo ◽  
Ectopic Expression ◽  
Explant Culture ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Supporting Cells ◽  
Minimal Damage

Hair cell (HC) regeneration is a promising therapy for permanent sensorineural hearing loss caused by HC loss in mammals. Atoh1 has been shown to convert supporting cells (SCs) to HCs in neonatal cochleae; its combinations with other factors can improve the efficiency of HC regeneration. To identify additional transcription factors for efficient Atoh1-mediated HC regeneration, here we optimized the electroporation procedure for explant culture of neonatal mouse organs of Corti and tested multiple transcription factors, Six2, Ikzf2, Lbh, Arid3b, Hmg20 a, Tub, Sall1, and Znf532, for their potential to promote Atoh1-mediated conversion of SCs to HCs. These transcription factors are expressed highly in HCs but differentially compared to the converted HCs based on previous studies, and are also potential co-reprograming factors for Atoh1-mediated SC-to-HC conversion by literature review. P0.5 cochlear explants were electroporated with these transcription factors alone or jointly with Atoh1. We found that Sox2+ progenitors concentrated within the lateral greater epithelial ridge (GER) can be electroporated efficiently with minimal HC damage. Atoh1 ectopic expression promoted HC regeneration in Sox2+ lateral GER cells. Transcription factors Tub and Znf532, but not the other six tested, promoted the HC regeneration mediated by Atoh1, consistent with previous studies that Isl1 promotes Atoh1-mediated HC conversionex vivo and in vivo and that both Tub and Znf532 are downstream targets of Isl1. Thus, our studies revealed an optimized electroporation method that can transfect the Sox2+ lateral GER cells efficiently with minimal damage to the endogenous HCs. Our results also demonstrate the importance of the Isl1/Tub/Znf532 pathway in promoting Atoh1-mediated HC regeneration.

scholarly journals Selective Ablation of Inner Hair Cells and Subsequent In-situ Hair Cell Regeneration in the Neonatal Mouse Cochlea

2021 ◽  
pp. 108275
Author(s):  
Mingyu Xia ◽  
Mingxuan Wu ◽  
Liping Zhao ◽  
Jiaoyao Ma ◽  
Wenyan Li ◽  
...  
Keyword(s):  
Hair Cell ◽  
Hair Cells ◽  
Neonatal Mouse ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Inner Hair Cells ◽  
Selective Ablation

A Review of Past and Present Hair Cell Regeneration Techniques

2007 ◽  
Vol 34 (Fall) ◽  
pp. 134-144 ◽  
Author(s):  
Anna Kharlamova ◽  
Nancy L. Aarts
Keyword(s):  
Hair Cell ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

Hair cell regeneration in the inner ear

1994 ◽  
Vol 111 (3) ◽  
pp. 281-301 ◽  
Author(s):  
T TSUE ◽  
E OESTERLE ◽  
E RUBEL
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Regeneration ◽  
Hair Cell Regeneration
Sign in / Sign up

Export Citation Format

Share Document