scholarly journals Insulin/IGF signaling and TORC1 promote vitellogenesis via inducing juvenile hormone biosynthesis in the American cockroach

Development ◽  
2020 ◽  
Vol 147 (20) ◽  
pp. dev188805
Author(s):  
Shiming Zhu ◽  
Fangfang Liu ◽  
Huanchao Zeng ◽  
Na Li ◽  
Chonghua Ren ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Molecular Mechanisms ◽  
Periplaneta Americana ◽  
Fat Body ◽  
Bovine Insulin ◽  
Molecular Techniques ◽  
American Cockroach ◽  
Nutritional Regulation ◽  
Juvenile Hormone Biosynthesis ◽  
Igf Signaling

ABSTRACTVitellogenesis, including vitellogenin (Vg) production in the fat body and Vg uptake by maturing oocytes, is of great importance for the successful reproduction of adult females. The endocrinal and nutritional regulation of vitellogenesis differs distinctly in insects. Here, the complex crosstalk between juvenile hormone (JH) and the two nutrient sensors insulin/IGF signaling (IIS) and target of rapamycin complex1 (TORC1), was investigated to elucidate the molecular mechanisms of vitellogenesis regulation in the American cockroach, Periplaneta americana. Our data showed that a block of JH biosynthesis or JH action arrested vitellogenesis, in part by inhibiting the expression of doublesex (Dsx), a key transcription factor gene involved in the sex determination cascade. Depletion of IIS or TORC1 blocked both JH biosynthesis and vitellogenesis. Importantly, the JH analog methoprene, but not bovine insulin (to restore IIS) and amino acids (to restore TORC1 activity), restored vitellogenesis in the neck-ligated (IIS-, TORC1- and JH-deficient) and rapamycin-treated (TORC1- and JH-deficient) cockroaches. Combining classic physiology with modern molecular techniques, we have demonstrated that IIS and TORC1 promote vitellogenesis, mainly via inducing JH biosynthesis in the American cockroach.

scholarly journals Insulin/IGF signaling and TOR promote vitellogenesis via inducing juvenile hormone biosynthesis

2020 ◽  
Author(s):  
Shiming Zhu ◽  
Fangfang Liu ◽  
Huanchao Zeng ◽  
Na Li ◽  
Chonghua Ren ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Molecular Mechanisms ◽  
Periplaneta Americana ◽  
Fat Body ◽  
Bovine Insulin ◽  
Molecular Techniques ◽  
American Cockroach ◽  
Nutritional Regulation ◽  
Juvenile Hormone Biosynthesis ◽  
Igf Signaling

ABSTRACTVitellogenesis, including vitellogenin (Vg) production in the fat body and Vg uptake by maturing oocytes, is of great importance for the successful reproduction of adult females. The endocrinal and nutritional regulation of vitellogenesis differs distinctly in insects. Here, the complex crosstalk between juvenile hormone (JH) and the two nutrient sensors, insulin/IGF signaling (IIS) and target of rapamycin (TOR), was investigated to elucidate the molecular mechanisms regulating vitellogenesis in the American cockroach, Periplaneta americana. Our data showed that a block of JH biosynthesis or JH action arrested vitellogenesis, partially by inhibiting the expression of doublesex (Dsx), a key transcription factor gene involved in the sex determination cascade. Depletion of IIS and TOR blocked both JH biosynthesis and vitellogenesis. Importantly, the JH analog methoprene, but not bovine insulin (to restore IIS) and amino acids (to restore TOR activity), restored vitellogenesis in the neck-ligated (both nutrient- and JH-deficient) cockroaches. Combining classic physiology with modern molecular techniques, we have demonstrated that JH signaling alone is able to induce vitellogenesis and thus ovarian maturation. By contrast, IIS and TOR do not induce vitellogenesis independent of JH, the nutrient sensors promote vitellogenesis in an indirect manner via activating JH biosynthesis.

INHIBITORS OF CARBONIC ANHYDRASE IN THE AMERICAN COCKROACH, PERIPLANETA AMERICANA (L.)

1956 ◽  
Vol 34 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Ann D. Anderson ◽  
Ralph B. March
Keyword(s):  
Carbonic Anhydrase ◽  
Periplaneta Americana ◽  
Fat Body ◽  
Aqueous Media ◽  
Carbonic Anhydrase Activity ◽  
American Cockroach ◽  
Tissue Homogenates ◽  
Organic Insecticides ◽  
Cell Fragments

Carbonic anhydrase activity has been demonstrated in vitro in preparations of the head, fat body, and gut of the American cockroach, Periplaneta americana (L.), and in the adult housefly, Musca domestica L. The insect factor, which is soluble in aqueous media and can be separated from the particulate cell fragments of insect tissue homogenates is heat labile and sensitive to cyanide inactivation. It is strongly inhibited by sulphanilamide, p-aminoethylphenyl-sulphonamide, and p-chlorphenylsulphonamide. No inhibition has been found with N-substituted sulphonamides or with any of the organic insecticides examined, including DDT, lindane, dieldrin, nicotine, rotenone, pyrethrins, and para-oxon. Sensitivity of carbonic anhydrase to sulphonamides having an intact—SO2NH2 group is also characteristic of mammalian preparations. The data indicate that inhibition of insect carbonic anhydrase cannot be an important factor in the mode of action of DDT or other organic insecticides.

scholarly journals Isolation and structure of a novel charged member of the red–pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera)

1990 ◽  
Vol 269 (2) ◽  
pp. 309-313 ◽  
Author(s):  
G Gäde ◽  
H Wilps ◽  
R Kellner
Keyword(s):  
Periplaneta Americana ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Reversed Phase ◽  
American Cockroach ◽  
Red Pigment ◽  
Adipokinetic Hormone ◽  
Migratory Locust ◽  
Corpora Cardiaca ◽  
Adipokinetic Hormone Family

A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named ‘Phormia terraenovae hypertrehalosaemic hormone’ (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.

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