Extension of Protein Functions by the Incorporation of Nonnatural Amino Acids

1999 ◽  
Vol 72 (7) ◽  
pp. 1409-1425 ◽  
Author(s):  
Masahiko Sisido ◽  
Takahiro Hohsaka
Keyword(s):  
Amino Acids ◽  
Nonnatural Amino Acids ◽  
Protein Functions

scholarly journals In vitro incorporation of nonnatural amino acids into protein using tRNACys-derived opal, ochre, and amber suppressor tRNAs

RNA ◽  
2010 ◽  
Vol 16 (8) ◽  
pp. 1660-1672 ◽  
Author(s):  
J. Gubbens ◽  
S. J. Kim ◽  
Z. Yang ◽  
A. E. Johnson ◽  
W. R. Skach
Keyword(s):  
Amino Acids ◽  
Nonnatural Amino Acids ◽  
Amber Suppressor

Incorporation of Two Different Nonnatural Amino Acids Independently into a Single Protein through Extension of the Genetic Code

1999 ◽  
Vol 121 (51) ◽  
pp. 12194-12195 ◽  
Author(s):  
Takahiro Hohsaka ◽  
Yuki Ashizuka ◽  
Hiroshi Sasaki ◽  
Hiroshi Murakami ◽  
Masahiko Sisido
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Nonnatural Amino Acids ◽  
Single Protein

scholarly journals Mapping the Specific Amino Acid Residues That Make Hamster DPP4 Functional as a Receptor for Middle East Respiratory Syndrome Coronavirus

2016 ◽  
Vol 90 (11) ◽  
pp. 5499-5502 ◽  
Author(s):  
Neeltje van Doremalen ◽  
Kerri L. Miazgowicz ◽  
Vincent J. Munster
Keyword(s):  
Amino Acids ◽  
Middle East ◽  
Case Fatality ◽  
Small Animal ◽  
Middle East Respiratory Syndrome ◽  
The Novel ◽  
Amino Acid Residues ◽  
Specific Amino Acid ◽  
Dipeptidyl Peptidase 4 ◽  
Protein Functions

ABSTRACTThe novel emerging coronavirus Middle East respiratory syndrome coronavirus (MERS-CoV) binds to its receptor, dipeptidyl peptidase 4 (DPP4), via 14 interacting amino acids. We previously showed that if the five interacting amino acids which differ between hamster and human DPP4 are changed to the residues found in human DPP4, hamster DPP4 does act as a receptor. Here, we show that the functionality of hamster DPP4 as a receptor is severely decreased if less than 4 out of 5 amino acids are changed.IMPORTANCEThe novel emerging coronavirus MERS-CoV has infected >1,600 people worldwide, and the case fatality rate is ∼36%. In this study, we show that by changing 4 amino acids in hamster DPP4, this protein functions as a receptor for MERS-CoV. This work is vital in the development of new small-animal models, which will broaden our understanding of MERS-CoV and be instrumental in the development of countermeasures.

Incorporation of Nonnatural Amino Acids Into Proteins

2004 ◽  
Vol 73 (1) ◽  
pp. 147-176 ◽  
Author(s):  
Tamara L. Hendrickson ◽  
Valérie de Crécy-Lagard ◽  
Paul Schimmel
Keyword(s):  
Amino Acids ◽  
Nonnatural Amino Acids

Radiohalogenated nonnatural amino acids as PET and SPECT tumor imaging agents

2011 ◽  
Vol 32 (4) ◽  
pp. 868-905 ◽  
Author(s):  
Jonathan McConathy ◽  
Weiping Yu ◽  
Nachwa Jarkas ◽  
Wonewoo Seo ◽  
David M. Schuster ◽  
...  
Keyword(s):  
Amino Acids ◽  
Tumor Imaging ◽  
Imaging Agents ◽  
Nonnatural Amino Acids

scholarly journals Using Nonnatural Amino Acids to Control Metal-Coordination Number in Three-Stranded Coiled Coils

2006 ◽  
Vol 118 (18) ◽  
pp. 2930-2934 ◽  
Author(s):  
Kyung-Hoon Lee ◽  
Chris Cabello ◽  
Lars Hemmingsen ◽  
E. Neil G. Marsh ◽  
Vincent L. Pecoraro
Keyword(s):  
Amino Acids ◽  
Coordination Number ◽  
Coiled Coils ◽  
Metal Coordination ◽  
Nonnatural Amino Acids
Sign in / Sign up

Export Citation Format

Share Document