Studies on the pectic substances of plant cell walls. Part II. Fractionation of polysaccharides and acidic oligosaccharides formed on partial acid hydrolysis of pectin.

A particulate enzyme preparation made from suspension-cultured dwarf-French-bean (Phaseolus vulgaris) cv. Canadian Wonder cells was shown to incorporate xylose from UDP-D-[14C]xylose into polysaccharide. The reaction was dependent upon the presence of UDP-D-glucose and was stimulated, and apparently protected, by GDP-D-glucose and GDP-D-mannose, though neither was able to replace UDP-D-glucose as a glycosyl donor. The product of the reaction was identified as xyloglucan by analysis of products of enzyme breakdown and acid hydrolysis. Mr determination after proteinase K digestion indicated that the nascent xyloglucan is closely associated with protein. Preincubation of the enzyme with UDP-D-glucose stimulated incorporation from UDP-D-[14C]xylose, suggesting an ‘imprecise’ mechanism of biosynthesis, as defined by Waldron & Brett [(1985) in Biochemistry of Plant Cell Walls (Brett, C. T. & Hillman, J. R., eds.) (SEB Semin. Ser. 28), pp. 79-97, Cambridge University Press, Cambridge].

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Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Biotechnology for Biofuels ◽

10.1186/s13068-017-0703-6 ◽

2017 ◽

Vol 10 (1) ◽

Cited By ~ 8

Author(s):

Johnnie A. Walker ◽

Sivakumar Pattathil ◽

Lai F. Bergeman ◽

Emily T. Beebe ◽

Kai Deng ◽

...

Keyword(s):

Plant Cell ◽

Cell Walls ◽

Glycoside Hydrolase ◽

Plant Cell Walls ◽

Glycome Profiling ◽

Hydrolysis Of

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Structure and composition of the alkali-insoluble cell wall fraction of Coprinus macrorhizus var. microsporus

Canadian Journal of Biochemistry ◽

10.1139/o80-021 ◽

1980 ◽

Vol 58 (2) ◽

pp. 147-153 ◽

Cited By ~ 5

Author(s):

Carey B. Bottom ◽

Donald J. Siehr

Keyword(s):

Amino Acids ◽

Cell Wall ◽

Acid Hydrolysis ◽

Cell Walls ◽

Cell Wall Fraction ◽

Enzymic Hydrolysis ◽

Methylation Analysis ◽

Partial Acid Hydrolysis ◽

The Core ◽

Hydrolysis Of

The alkali-insoluble (R-) fraction from the cell walls of Coprinus macrorhizus var. microsporus is a highly branched glucan, containing α-(1 → 4), β-(1 → 3), and β-(1 → 6) linkages as shown by methylation, partial acid hydrolysis, and enzymic hydrolysis. The α-(1 → 4)-linked segments are joined by occasional β-(1 → 3) links as suggested by the identification of 2-O-α-glucopyranosyl erythritol in the hydrolysate of the reduced, periodate-oxidized glucan. Hydrolysis of the permethylated glucan gave nearly equimolar amounts of 2,4-di- and 2,3-di-O-methyl-D-glucose. Methylation analysis of the residue from enzymic hydrolysis, the "CORE-fraction," indicated the presence of glucose residues in this fraction linked through positions O1, O3, O4, and O6. Hydrolysates of the R-fraction contained mannose, glucosamine, and amino acids in addition to glucose.

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