Individual Identification using The Multiplex PCR with Microsatellite Markers in Swine

2013 ◽  
Vol 37 (4) ◽  
pp. 205-211
Author(s):  
Lee-Kung Kima ◽  
◽  
Chang-Min Parka ◽  
Sun-Ae Park ◽  
Seung-Chang Kim ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr ◽  
Individual Identification

scholarly journals Polymorphic DNA microsatellite markers for forensic individual identification and parentage analyses of seven threatened species of parrots (family Psittacidae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2416 ◽  
Author(s):  
Catherine Jan ◽  
Luca Fumagalli
Keyword(s):  
Microsatellite Markers ◽  
Threatened Species ◽  
Captive Breeding ◽  
Genomic Library ◽  
Habitat Destruction ◽  
Individual Identification ◽  
Illegal Trade ◽  
The Mean ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

The parrot family represents one of the bird group with the largest number of endangered species, as a result of habitat destruction and illegal trade. This illicit traffic involves the smuggling of eggs and animals, and the laundering through captive breeding facilities of wild-caught animals. Despite the huge potential of wildlife DNA forensics to determine with conclusive evidence illegal trade, current usage of DNA profiling approaches in parrots has been limited by the lack of suitable molecular markers specifically developed for the focal species and by low cross-species polymorphism. In this study, we isolated DNA microsatellite markers in seven parrot species threatened with extinction (Amazona brasiliensis,A. oratrix,A. pretrei,A. rhodocorytha,Anodorhynchus leari,Ara rubrogenysandPrimolius couloni). From an enriched genomic library followed by 454 pyrosequencing, we characterized a total of 106 polymorphic microsatellite markers (mostly tetranucleotides) in the seven species and tested them across an average number of 19 individuals per species. The mean number of alleles per species and across loci varied from 6.4 to 8.3, with the mean observed heterozygosities ranging from 0.65 to 0.84. Identity and parentage exclusion probabilities were highly discriminatory. The high variability displayed by these microsatellite loci demonstrates their potential utility to perform individual genotyping and parentage analyses, in order to develop a DNA testing framework to determine illegal traffic in these threatened species.

Characterization of 33 microsatellite markers and development of multiplex PCR for yellow-throated marten (Martes flavigula)

2017 ◽  
Vol 16 (4) ◽  
Author(s):  
Mu Yeong Lee ◽  
Hea Chang Moon ◽  
Hey Sook Jeon ◽  
Eui Geun Song ◽  
Donggul Woo ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Multiplex Pcr

Development of a New Microsatellite Markers for Individual Identification and Paternity Evaluation in Hanwoo

2020 ◽  
Vol 54 (4) ◽  
pp. 75-83
Author(s):  
Hye-Ran Kim ◽  
◽  
Ji-Woong Lee ◽  
Min-Jung Go ◽  
Jong-Eun Park ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Individual Identification

scholarly journals Individual Identification and Breed Allocation with Microsatellite Markers: An Evaluation in Indian Horses

2006 ◽  
Vol 20 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Rahul Behl ◽  
Jyotsna Behl ◽  
Neelam Gupta ◽  
S. C. Gupta ◽  
S. P. S. Ahlawat
Keyword(s):  
Microsatellite Markers ◽  
Individual Identification

A novel multiplex PCR assay for individual identification in the vulnerable Chinese Egret (Egretta eulophotes) using molted feathers

2015 ◽  
Vol 7 (3) ◽  
pp. 635-638
Author(s):  
Yufei Dai ◽  
Wenzhen Fang ◽  
Qingxian Lin ◽  
Xiaoping Zhou ◽  
Xiaolin Chen
Keyword(s):  
Multiplex Pcr ◽  
Individual Identification ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Egretta Eulophotes ◽  
Chinese Egret
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