scholarly journals Polymorphic DNA microsatellite markers for forensic individual identification and parentage analyses of seven threatened species of parrots (family Psittacidae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2416 ◽  
Author(s):  
Catherine Jan ◽  
Luca Fumagalli
Keyword(s):  
Microsatellite Markers ◽  
Threatened Species ◽  
Captive Breeding ◽  
Genomic Library ◽  
Habitat Destruction ◽  
Individual Identification ◽  
Illegal Trade ◽  
The Mean ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

The parrot family represents one of the bird group with the largest number of endangered species, as a result of habitat destruction and illegal trade. This illicit traffic involves the smuggling of eggs and animals, and the laundering through captive breeding facilities of wild-caught animals. Despite the huge potential of wildlife DNA forensics to determine with conclusive evidence illegal trade, current usage of DNA profiling approaches in parrots has been limited by the lack of suitable molecular markers specifically developed for the focal species and by low cross-species polymorphism. In this study, we isolated DNA microsatellite markers in seven parrot species threatened with extinction (Amazona brasiliensis,A. oratrix,A. pretrei,A. rhodocorytha,Anodorhynchus leari,Ara rubrogenysandPrimolius couloni). From an enriched genomic library followed by 454 pyrosequencing, we characterized a total of 106 polymorphic microsatellite markers (mostly tetranucleotides) in the seven species and tested them across an average number of 19 individuals per species. The mean number of alleles per species and across loci varied from 6.4 to 8.3, with the mean observed heterozygosities ranging from 0.65 to 0.84. Identity and parentage exclusion probabilities were highly discriminatory. The high variability displayed by these microsatellite loci demonstrates their potential utility to perform individual genotyping and parentage analyses, in order to develop a DNA testing framework to determine illegal traffic in these threatened species.

scholarly journals DNA Microsatellite Markers forSwartzia glazioviana(Fabaceae), a Threatened Species from the Brazilian Atlantic Forest

2016 ◽  
Vol 4 (2) ◽  
pp. 1500081 ◽  
Author(s):  
Janaína Spoladore ◽  
Vidal F. Mansano ◽  
Luan C. Dias de Freitas ◽  
Alexandre M. Sebbenn ◽  
Maristerra R. Lemes
Keyword(s):  
Microsatellite Markers ◽  
Atlantic Forest ◽  
Threatened Species ◽  
Brazilian Atlantic Forest ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

scholarly journals Isolation and Development of DNA Microsatellite Markers for the River Catfish (Mystus nemurus)

2007 ◽  
Vol 20 (1) ◽  
Author(s):  
B.P. HOH ◽  
S.S. SIRAJ ◽  
S.G. TAN ◽  
K. YUSOFF
Keyword(s):  
Microsatellite Markers ◽  
Genomic Library ◽  
Degenerate Primer ◽  
Specific Primer ◽  
Allelic Frequencies ◽  
Microsatellite Repeats ◽  
Anchored Pcr ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers ◽  
Mystus Nemurus

The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored PCR procedure. A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. From these, 20 specific primer pairs were designed and employed to characterize unrelated fishes from six populations of M. nemurus. Thirteen loci were found to be polymorphic. The allelic frequencies ranged from 0.007 to 0.800, while the levels of heterozygosity ranged from 0.0563 (MnBp5-1-115b) to 0.8714 (MnBp5-1-12a).

The way of value of Correlation of genomic DNA microsatellite loci and live weight of Chukchi reindeer

2020 ◽  
pp. 27-32
Author(s):  
V. Dodokhov ◽  
N. Pavlova ◽  
T. Rumyantseva ◽  
L. Kalashnikova
Keyword(s):  
Microsatellite Markers ◽  
Agricultural Production ◽  
Live Weight ◽  
Biodiversity Loss ◽  
Tribal Community ◽  
High Germination ◽  
Wide Range ◽  
The Mean ◽  
High Level ◽  
Dna Microsatellite

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

Verification of the identity of bovine semen using DNA microsatellite markers

1997 ◽  
Vol 140 (17) ◽  
pp. 446-449 ◽  
Author(s):  
J. L. Williams ◽  
A. P. Usha ◽  
B. G. D. Urquhart ◽  
M. Kilroy
Keyword(s):  
Microsatellite Markers ◽  
Bovine Semen ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers
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