scholarly journals 217 Observation of Microscopic Surface Change during Plastic Deformation of Polycrystalline metals by Laser-Scanning Microscope

2001 ◽  
Vol 2001.39 (0) ◽  
pp. 73-74
Author(s):  
Hualin Song ◽  
Takeji ABE ◽  
Ichiro SHIMIZU ◽  
Naoya TADA
2007 ◽  
Vol 340-341 ◽  
pp. 803-810 ◽  
Author(s):  
Hua Lin Song ◽  
Takeji Abe

The mi c ros copi c pl as t i c de format ion behavior of pol yc r ys t al l ine aluminum shee t dur ing uni axi al t ens ion i s exper iment al l y inves t iga t ed b y a confoc al l a s e r - s canning mi c ros cope. The gr ain rot at ion i s me asur ed f rom images of spec imen sur fa c e be fore and a f t e r deformat ion i s propos ed. Digi t al image proc es s ing t e chnique i s appl i ed to the sur f a c e gr ain image t aken by the CCD c ame ra . The exper iment al dat a obt ained f rom man y gr a ins a re s t a t i c al l y proce s s ed. I t i s shown that the gr ain rot at ion i s l a rge when the shape of gr ain i s clos e to a ci r cl e. Di s cus s ions a r e made on the r el at ion be twe en gra in rot at ion, s t ra ins of gr ains and va r ious f a ctor s af f e ct ing them, such as gr ain s i z e, gra in shape and s l ip- l ine angl e.


Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.


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