scholarly journals Influence of Hematocrit on Behavior of Ice Crystals and Human Red Blood Cells during Directional Solidification of Cell Suspensions.

1994 ◽  
Vol 60 (579) ◽  
pp. 3755-3761
Author(s):  
Hiroshi Ishiguro ◽  
Boris Rubinsky
Keyword(s):  
Red Blood Cells ◽  
Directional Solidification ◽  
Blood Cells ◽  
Cell Suspensions ◽  
Ice Crystals ◽  
Human Red Blood Cells

Application of new cryo-SEM to prove rapid freezing survival of unprotected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 812-813
Author(s):  
Seizo Fujikawa ◽  
Shinichi Ueda
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Isotonic Saline ◽  
Ice Crystals ◽  
Rapid Freezing ◽  
Continuous Observation ◽  
Copper Block ◽  
Cellular Survival ◽  
Human Red Blood Cells ◽  
Different Thickness

It has been suggested that rapid freezing, by which vitrification or intracellular freezing with ice crystals less than lOnm in diameter can be achieved, may preserve not only ultrastructure but also cellular function, although the definite evidence, especially in unprotected and highly hydrated biological materials, is not yet obtained. In this study, new cryo-SEM which allows continuous observation of same frozen samples by means of cryo-SEM and then by means of freeze-replicas, was used to prove the possibility of cellular survival by rapid freezing.Human red blood cells suspended in isotonic saline were used for materials. Samples with different thickness from 25μm to 200pm were loaded on holders and frozen by slamming against LHe cooled copper block by using metal contact machine (Meiwa Co. QF-5000). The entire frozen samples were carefully removed from the holder under liquid nitrogen. Some of the frozen samples were thawed rapidly and survival (% hemolysis) were determined.

scholarly journals Transbilayer distribution and mobility of phosphatidylinositol in human red blood cells.

1990 ◽  
Vol 265 (27) ◽  
pp. 16035-16038 ◽  
Author(s):  
P Bütikofer ◽  
Z W Lin ◽  
D T Chiu ◽  
B Lubin ◽  
F A Kuypers
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells
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